| The Role of Estrogen-Related Receptor Alpha in Steroidogenesis in the Breast |
01-Apr-2009 |
75 pages |
| Authors:
Linda Grasfeder; DUKE UNIV DURHAM NC
|
 | This project has focused on the potential role of Estrogen-Related Receptor alpha (ERRalpha) in regulating steroidogenesis. ERRalpha is an orphan nuclear receptor closely related to the Estrogen Receptors (ERs), and while its expression correlates with unfavorable biomarkers and poor prognosis for breast cancer, its function in breast cancer biology is not known. This project was initiated because we observed that a primary coactivator of ERRalpha, PGC-1alpha, induces hepatic gene expression ... |
|
| Role of PELP1 in EGFR-ER Signaling Crosstalk in Ovarian Cancer Cells |
Apr-2009 |
48 pages |
| Authors:
Ratna K Vadlamudi; TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
|
| Ovarian cancer is an endocrine-related cancer, but it remains unknown which hormone-regulated mechanisms are critical in ovarian cancer pathogenesis. In this study, we have identified nuclear hormonal receptor coregulator, Proline-, glutamic acid-, and leucine-rich protein-1 (PELP1/MNAR) as a novel proto-oncogene for ovarian cancer and showed that PELP1/MNAR is 2 to 3 fold overexpressed in 60% of ovarian tumors. To examine the significance of PELP1/MNAR in ovarian cancer progression, we have ... |
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| Interactions Between IGFBP-3 and Nuclear Receptors in Prostate Cancer Apoptosis |
Jan-2009 |
25 pages |
| Authors:
Kuk-Wha Lee; MATTEL CHILDREN'S HOSPITAL LOS ANGELES CA
|
| IGFBP-3 is a potent inducer of apoptosis in both androgen-dependent and androgen-independent prostate cancer lines. When the nuclear receptor RXRalpha was described as an unexpected intracellular binding partner for IGFBP-3 and effects on DNA transcription were demonstrated, rapid effects of IGFBP-3 on programmed cell death (apoptosis) still could not be explained. These rapid effects on apoptosis were clarified when I hypothesized that IGFBP-3 was a biological signal for Nur77 nuclear ... |
|
| Automation and Preclinical Evaluation of a Dedicated Emission Mammotomography System for Fully 3-D Molecular Breast Imaging |
Oct-2008 |
24 pages |
| Authors:
Spencer J Cutler; DUKE UNIV DURHAM NC
|
| The overall objective of this proposal is to fully automate and optimize the performance of a 3-D dedicated emission mammotomography system for enhanced semi-automated clinical testing. In the second year of study, automated laser-guided contouring hardware and software were successfully implemented, and a final design is in progress. An observer-based direct comparison of 3D dedicated SPECT and 2D planar scintimammography was also conducted. Measuring estimated volumes by voxel integration of ... |
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| Role of PELP1 in EGFR-ER Signaling Crosstalk in Ovarian Cancer Cells |
Apr-2008 |
71 pages |
| Authors:
Ratna K Vadlamudl; TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
|
| Emerging evidence suggests that nuclear receptor (NR) coregulators have potential to act as master genes and their deregulation can promote oncogenesis. Proline-, glutamic acid-, and leucine-rich protein-1 (PELP1/MNAR) is a novel NR coregulator. IHC studies using human ovarian cancer tissue arrays (n=123) showed that PELP1/MNAR is 2 to 3 fold over expressed in 60% of ovarian tumors To examine the significance of PELP1/MNAR in ovarian cancer progression, we have generated ... |
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| The Role of Nuclear Receptor Coactivator A1B1 in Growth Factor-Mediated Mammary Tumorigenesis |
01-Mar-2008 |
13 pages |
| Authors:
Mark P Fereshteh; GEORGETOWN UNIV WASHINGTON DC
|
| AIBI (Amplified In Breast Cancer I) is a nuclear receptor coactivator whose gene is amplified in 5-10% of breast cancers and both the mRNA and protein are overexpressed in 30% of breast tumors. In vitro studies show that AIBI plays a significant role in estrogen and IGF-1-induced cell proliferation. Germline knockout of the AIBI gene leads to reduced somatic growth abnormal reproductive function and reduced mammary gland development. Knockout of ... |
|
| Interactions between IGFBP-3 and Nuclear Receptors in Prostate Cancer Apoptosis |
01-Jan-2008 |
36 pages |
| Authors:
Kuk-Wha Lee; CALIFORNIA UNIV LOS ANGELES
|
| IGFBP-3 is a potent inducer of apoptosis in both androgen-dependent and androgen-independent prostate cancer lines. When the nuclear receptor RXRaIpha was described as an unexpected intracellular binding partner for IGFBP-3 and effects on DNA transcription were demonstrnted rapid effects of IGFBP-3 on programmed cell death (apoptosis) still could not be explained. These rapid effects on apoptosis were clarified when I hypothesized that IGFBP-3 was a biological signal for Nur77 nuclear ... |
|
| Automation and Preclinical Evaluation of a Dedicated Emission Mammotomography System for Fully 3-D Molecular Breast Imaging |
OCT 2007 |
24 pages |
| Authors:
Spencer J. Cutler; DUKE UNIV DURHAM NC
|
| The overall objective of this proposal is to fully automate and optimize the performance of a 3-D dedicated emission mammotomography system for enhanced semi-automated clinical testing. A retrospective study of 103 clinical MRI uncompressed breast scans was conducted to create surface renderings of the uncompressed breasts and analyze how to adapt existing acquisition orbits for varying breast shapes. Laser ribbon ranging sensors and associated hardware to fully automate the radius ... |
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| ERR Gamma: Does an Orphan Nuclear Receptor Link Steroid Hormone Biogenesis to Endocrine Resistance? |
01 SEP 2007 |
39 pages |
| Authors:
Rebecca B. Riggins; GEORGETOWN UNIV WASHINGTON DC
|
| Estrogen-related receptor gamma (ERR gamma) is an orphan nuclear receptor with structural similarities to ER alpha and ER beta (1). In addition to its ability to transactivate classical and imperfect estrogen response elements (EREs) ERR gamma is a potent activator of transcription from steroidogenic factor-1 (SF-1) response elements (SF-1REs). Many genes regulated by SF-1REs control key aspects of cholesterol and fatty acid synthesis important not only for generation of the ... |
|
| Development and Characterization of Novel Volumetric Acquisition Orbits with an Application Specific Emission Tomograph for Improved Breast Cancer Detection |
AUG 2007 |
17 pages |
| Authors:
Kristy Perez; Caryl N. Brzymialkiewicz; DUKE UNIV DURHAM NC
|
| The overall goal of this work was to develop, implement, and evaluate novel three-dimensional acquisition orbits for a dedicated emission mammotomography system. The system can revolve around a pendant, uncompressed breast with fully three dimensional orbital trajectories, which allow for chest wall and axillary imaging. Further optimization and characterization of the system's flexible trajectories and resulting reconstructed images continues. Upon the recommendations from this and prior work, one patient study ... |
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| Targeting of the Nuclear Receptor Coativator Isoform Delta 3aib1 in Breast Cancer. Addendum |
JUL 2007 |
12 pages |
| Authors:
Christopher Chien; GEORGETOWN UNIV WASHINGTON DC
|
| AIB1 which stands for "Amplified in Breast Cancer" codes for a protein that is a member of the steroid receptor coactivator (SRC) family. AIB1 is amplified in approximately 5-10% of breast cancers and the mRNA and protein overexpressed in >30% of breast cancers. AIB1 interacts with a super family of ligand activated nuclear receptors to potentiate transcriptional activity leading to upregulation of downstream target gene expression. An important finding was ... |
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| The Role of Nuclear Receptor Coactivator A1B1 in Growth Factor-Mediated Mammary Tumorigenesis |
01 MAR 2007 |
13 pages |
| Authors:
Mark P. Fereshteh; GEORGETOWN UNIV WASHINGTON DC
|
| AIB1 (Amplified In Breast Cancer 1) is a nuclear receptor coactivator whose gene is amplified in 5-10% of breast cancers and both the mRNA and protein are overexpressed in ~30% of breast tumors. In vitro studies show that AIB1 plays a significant role in estrogen and IGF-1-induced cell proliferation. Germline knockout of the AIB1 gene leads to reduced somatic growth, abnormal reproductive function and reduced mammary gland development. Knockout of ... |
|
| Prostate Cancer Aggressiveness Gene in Hereditary Prostate Cancer |
MAR 2007 |
21 pages |
| Authors:
Kathleen Cooney; MICHIGAN UNIV ANN ARBOR
|
| This is the final report of our CDMRP-funded research grant. Our study comprised of two research aims to examine the role of EZH2 and prostate cancer. In the first of these aims, we have explored and developed data indicating that a polymorphism in EZH2, Asp146His, may contribute to aggressive prostate cancer, and we are currently conducting validation studies to determine if our findings may be used to predict whether men ... |
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| Estrogen-Related Receptor alpha (ERR (alpha))-Coactivator Interactions as Targets for Discovery of New Anti-Breast Cancer Therapeutics |
MAR 2007 |
13 pages |
| Authors:
Richard R. Burgess; WISCONSIN UNIV-MADISON
|
| The steroid nuclear receptor estrogen receptor alpha (ERalpha) is the primary target of current breast cancer therapies, which block ERo activation or estrogen synthesis. Estrogen-related receptor alpha (ERRalpha), a protein with high sequence similarity to ERalpha, has functional similarity to ERalpha in certain breast cancer cell types; though unlike ERalpha, ERRalpha acts independently of steroid ligand. We hypothesize that its activity may be due in part to its interaction with ... |
|
| Understanding the Apoptotic Functions of IGFBP-3 in Prostate Cancer |
NOV 2006 |
33 pages |
| Authors:
Laura Cobb; Pinchas Cohen; CALIFORNIA UNIV LOS ANGELES
|
| The IGF axis is known to play an important role in the epidemiology of many tumors. IGFBP-3 promotes apoptosis in cancer cells by both IGF-dependent and -independent mechanisms. We have previously shown that IGF3P-3 is rapidly internalized and localized to the nucleus where its interactions with the nuclear receptor RXRo are important in apoptosis induction. We demonstrate that phosphorylation of IGFBP-3 (S156) by DNA-PK enhances its nuclear accumulation and is ... |
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| Nuclear Receptor Interactions in Breast Cancer: The Role of Kinase Signaling Pathways |
JUL 2006 |
9 pages |
| Authors:
Filippa Pettersson; MCGILL UNIV MONTREAL (QUEBEC)
|
| Retinoids and rexinoids are vitamin A derivatives, which cause growth inhibition and/or apoptosis in various cell types, including some breast cancer cells. Retinoids bind and activate the nuclear receptor RAR, whereas rexinoids specifically bind to the related receptor RXR. While retinoids like RA inhibit the growth of estrogen receptor (ER) positive and not ER negative breast cancer cells, rexinoids appear to have activity in both ER positive and ER negative ... |
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| The Regulation of Nuclear Receptor Coactivator SRC-3 Activity Through Membrane Receptor Mediated Signaling Pathways |
MAY 2006 |
18 pages |
| Authors:
Ping Yi; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| SRC-3/AIB1 is a steroid receptor coactivator with potent growth promoting activity. It is overexpressed in a number of cancers and has been shown to have oncogenic potential. An important mechanism for the regulation of SRC-3 coactivator activity is its site-specific phosphorylation status which is controlled by extracellular signals that stimulate specific protein kinase signaling pathways. It is well known that protein kinases such as aPKC are frequently overexpressed in cancers ... |
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| Estrogen-Related Receptor Alpha (ERRa)-Coactivator Interactions as Targets for Discovery of New Anti-Breast Cancer Therapeutics |
MAR 2006 |
12 pages |
| Authors:
Richard R. Burgess; WISCONSIN UNIV-MADISON
|
| The nuclear receptor estrogen receptor alpha (ERalpha) is a primary focus of the therapy of breast cancers. ERalpha-positive breast cancers have traditionally been treated with the anti-estrogen tamoxifen, or with inhibitors of aromatase. ERalpha-negative breast cancers do not respond to anti-estrogen treatment; instead, current therapeutics, such as Herceptin, have focused on the transmembrane tyrosine kinase receptor ErbB2 (HER2) because it is often overexpressed in ERalpha-negative tumors and correlates with a ... |
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| Nanoprobe Directed Tumor Imaging Using pH Activated Peptides as Contrast Agent Carriers |
MAR 2006 |
13 pages |
| Authors:
John E. Mata; Scout Gustafson; OREGON STATE UNIV CORVALLIS
|
| We characterized a class of modified peptides designed to activate in the extracellular space of tumors in which pH is below 7.0 in cell culture and in tumor bearing mice. These "molecular engines" change shape in a low pH environment, become lipophilic, and embed into the plasma membrane. Our studies suggest that a molecular engine with a pT of 6.8-6.9 will deliver an 8-carboxyfluoroscein ligand to the surface of tumor ... |
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| Anticar Inhibitors of AR-Mediated Gene Expression |
NOV 2005 |
19 pages |
| Authors:
Blake R. Peterson; PENNSYLVANIA STATE UNIV UNIVERSITY PARK
|
| New drugs that halt the progression of prostate cancers are urgently needed. Because many prostate cancers require the androgen dihydrotestosterone to proliferate, antiandrogens such as casodex (bicalutamide) are often the first line therapy for treatment of this disease. However, this drug and other clinically employed antiandrogens generally suffer from low affinity for the androgen receptor (AR), low selectivity across the nuclear hormone receptor superfamily, and do not achieve complete androgen ... |
|
| Nuclear Receptor Interactions in Breast Cancer: The Role of Kinase Signaling Pathways |
JUL 2005 |
20 pages |
| Authors:
Filippa Pettersson; MCGILL UNIV MONTREAL (QUEBEC) UPPER ATMOSPHERE CHEMISTRY GROUP
|
| Retinoids are vitamin A derivatives, which cause growth inhibition, differentiation and/or apoptosis in various cell types, including some breast cancer cells. In general, estrogen receptor (ER)-positive cells are retinoic acid (RA) sensitive, whereas ER-negative cells are resistant. I have showed that ER-negative MDA-MB-231 cells and MDA-MB-468 cells are strongly growth inhibited by retinoids in combination with PKC inhibition, and inhibition of PKC in particular. In this report, I show that ... |
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| Coactivator Requirements for Serm Action |
JUN 2005 |
24 pages |
| Authors:
Erika K. Keeton; DANA-FARBER CANCER INST BOSTON MA
|
| Estrogen receptor alpha- (ER(alpha)) mediates the effects of estrogens in breast cancer development and growth via transcriptional regulation of target genes. Tamoxifen can antagonize ER(alpha) activity and has been used in breast cancer therapy. The molecular determinants of tamoxifen action are not completely understood, but the availability of ER coregulators is thought to play a role. Tamoxifen-bound ER(alpha) associates with nuclear receptor corepressor (N-CoR) and silencing mediator for retinoid and ... |
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| Investigation of Metastatic Breast Tumor Heterogeneity and Progression Using Dual Optical/SPECT Imaging |
MAY 2005 |
39 pages |
| Authors:
Peter P. Antich; Anca Constantinescu; Matthew Lewis; Ralph Mason; Edmond Richer; TEXAS UNIV AT DALLAS SOUTHWESTERN MEDICAL CENTER
|
| The goal of our project is to image tumor growth, metastatic development and vascular changes, both to characterize tumor dynamics during growth for application in diagnostic and prognostic imaging. and to aid in the development of new therapies by dissecting the effects of treatment. The technical goal for the first year has been to develop a device to image the whole animal by nuclear medicine and optical techniques. Ultimately, we ... |
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| Nuclear Imaging for Assessment of Prostate Cancer Gene Therapy |
APR 2005 |
7 pages |
| Authors:
Dongfeng Pan; VIRGINIA UNIV CHARLOTTESVILLE
|
| Combination of the cytotoxic viral thymidine kinase (tk) and the prodrug, acyclovir (ACV) has been reported to inhibit the growth of the C4-2 tumor, a subline of LNCaP. However, it remains unsolved to non-invasively detect the in vivo distribution, expression and persistence of the toxic gene as well as to evaluate the therapeutic effect. In this project, we will develop a nuclear gene imaging approach to assist the cytotoxic gene ... |
|
| Targeting of the Nuclear Receptor Coactivator Isoform Delta3Air in Breast Cancer |
MAR 2005 |
21 pages |
| Authors:
Aparna Mani; GEORGETOWN UNIV WASHINGTON DC MEDICAL CENTER
|
| Of particular interest to breast cancer was the discovery that an area of chromosome 20q, known to be frequently amplified in breast cancer, harbored the gene for AIB1. AIB1 which stands for "Amplified in Breast cancer" codes for a protein which is a member of the steroid receptor coactivator (SRC) family. AIB1 is amplified in approximately 5-10% of breast cancers and the mRNA and protein overexpressed in >30% of breast ... |
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| Network Tomography |
24 JAN 2005 |
11 pages |
| Authors:
Franklin Gavilanez; Carlos Berenstein; John Baras; MARYLAND UNIV COLLEGE PARK INST FOR SYSTEMS RESEARCH
|
| While conventional tomography is associated to the Radon transform in Euclidean spaces, electrical impedance tomography or EIT is associated to the Radon transform in the hyperbolic plane. We discuss some recent work on network tomography that can be associated to a problem similar to EIT on graphs and indicate how in some sense it may be also associated to the Radon transform on trees. |
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| Role of Nuclear Receptor Cofactors in Hormone Refractory Prostate Cancer |
JAN 2005 |
10 pages |
| Authors:
Charlie D. Chen; CALIFORNIA UNIV LOS ANGELES
|
| Previously we discovered that a modest increase in androgen receptor (AR) expression is sufficient and necessary for hormone refractory (HR) prostate cancer progression. Together with published results of common AR overexpression in HR clinical tumor samples, our results indicate that AR overexpression is a cause for HR disease. We also demonstrated that the canonical transcriptional activity of AR is required in this process. Since the AR transcriptional function is mediated ... |
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| PET Radiotracers for Imaging the Proliferation Status of Breast Tumors |
DEC 2004 |
75 pages |
| Authors:
Robert H. Mach; WASHINGTON UNIV ST LOUIS MO
|
| The goal of this research project is to develop radiotracers for imaging the proliferative status of breast tumors using the noninvasive imaging technique, Positron Emission Tomography (PET). The strategy taken involves developing radiotracers having a high affinity and selectivity for the sigma (sub 2) receptor, which has been shown to be a useful receptor-based biomarker of proliferation in breast tumor cells growing both in vitro and in vivo. During the ... |
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| Role of a Novel Splice Variant of the Steroid Receptor Coactivator AIB1 in Breast Cancer |
AUG 2004 |
24 pages |
| Authors:
Anna T. Riegel; GEORGETOWN UNIV WASHINGTON DC
|
| In this proposal we have investigated the hypothesis that overexpression of a novel truncated version of AIBl (Delta3AIBl) that we have found to be overexpressed in breast cancer is important for tumor development by impacting upon nuclear hormone receptor function. We have examined the hypothesis that the novel AIBl variant has an altered function that changes its interaction with nuclear receptors such as the estrogen or progesterone receptor. We propose ... |
|
| Intraoperative Imaging for Sentinel Lymph Nodes |
AUG 2004 |
19 pages |
| Authors:
Carmen M. Greene; John N. Aarsvold; Nolan Hertel; GEORGIA INST OF TECH ATLANTA
|
| The main objective of this investigation is to determine the feasibility and value of the use of a small field of view camera gamma camera intraoperatively for sentinel lymph node (SLN) localization in breast cancer patients. A camera with a 5 in. x 5 in. field of view (FOV) is being investigated. Clinical experience with 14 subjects using the 1 in. x 1 in. FOV and 15 subjects using the ... |
|
| Functional Estrogen Deprivation by activating the Orphan Nuclear Receptor PXR |
AUG 2004 |
36 pages |
| Authors:
Wen Xie; PITTSBURGH UNIV PA
|
| Estrogens are required for mammary epithelial proliferation and are prerequisite for breast cancer formation. Accordingly, strategies to down- regulate estrogen activity have been proven effective to treat and prevent breast cancer. A major pathway to deactivate estrogens is through estrogen sulfation mediated by the phase II sulfotransferases (SULT). SULT is expressed in normal mammary epithelial cells but its expression and activities are significantly lower in many breast cancer cells, suggesting ... |
|
| The Role of PRIP in Breast Cancer |
JUL 2004 |
51 pages |
| Authors:
Yijun Zhu; Janardan K. Reddy; NORTHWESTERN UNIV EVANSTON IL
|
| Estrogen plays an important role in the normal breast and breast cancer development. Estrogens exert their cellular effects through ER that is a member of nuclear receptor superfamily. PRIP (Peroxisome proliferator receptor interacting protein) is a nuclear receptor coactivator that is amplified and overexpressed in breast cancer. The proposal was to investigate how the PRIP dysregulation contributes to abnormal growth and neoplastic development of breast. During the 3-year period, we ... |
|
| New Approaches in SPECT Breast Imaging |
JUL 2004 |
23 pages |
| Authors:
Kelly Braun; R. Jaszczak; K. Bobkov; J. Bowsher; DUKE UNIV DURHAM NC
|
| The primary objective of this research is to develop new techniques in single photon emission computed tomography (SPECT) breast cancer imaging providing improved detection and characterization of early stage breast cancer. Projection data were acquired by implementing a tiltable SPECT system on a breast and torso phantom. The data were reconstructed with both an ordered subset expectation maximization (OSEM) algorithm and a filtered backprojection (FBP) algorithm with a ramp filter. ... |
|
| Orphan Receptor TR3/nur77 and Apoptosis in Prostate Cancer Cells |
JUL 2004 |
44 pages |
| Authors:
Xiao-kun Zhang; BURNHAM INST LA JOLLA CA
|
| TR3 (also called Nur77 or NGFI-B), an immediate-early response gene and an orphan member of the nuclear receptor superfamily, regulates both survival and death of prostate cancer cells. It is overexpressed in prostate tumor and acts in the nucleus to promote tumor cell growth. In response to certain apoptotic stimuli, TR3 migrates from the nucleus to the cytoplasm where it targets mitochondria to induce cytochrome C release and apoptosis. Translocation ... |
|
| Multiparametric Evaluation of Marker Expression |
JUN 2004 |
32 pages |
| Authors:
Awtar Ganju-Krishan; MIAMI UNIV FL
|
| This project sought to develop flow cytometric methods for monitoring of nuclear hormone receptor expression, DNA content and nuclear volume in human breast tumors. Data was collected and published on estrogen, androgen and vitamin D receptor expression in breast tumors from female and male patients. In a parallel study, we have sought correlation between expression of nuclear hormone receptors and patient characteristics of breast tumors. A manuscript based on expression ... |
|
| Role of GIF1 (GADD45 Interacting Factor 1) in Breast Cancer |
JUN 2004 |
70 pages |
| Authors:
Insoo Bae; GEORGETOWN UNIV WASHINGTON DC
|
| Gadd45, a DNA damage inducible gene, plays role(s) in DNA repair and regulation of cell proliferation. Using yeast two hybrid system, we identified a novel gene whose products interact with GADD45 alpha and named it as GIF1 (Gadd45 alpha Interacting Factor 1). Alternative name of GIF1 is CRIF1 (=CR6 interacting factor 1, CR6=Gadd45 gamma). During the first year of finding period, we reported that a physical interaction of Gadd45 family ... |
|
| Nuclear Imaging for Assessment of Prostate Cancer Gene Therapy |
APR 2004 |
7 pages |
| Authors:
Dongfeng Pan; VIRGINIA UNIV CHARLOTTESVILLE
|
| Combination of the cytotoxic viral hymidine kinase (tk) and the prodrug, acyclovir (ACV) has been reported to inhibit the growth of the C4-2 tumor, a subline of LNCaP. However, it remains unsolved to non-invasively detect the in vivo distribution, expression and persistence of the toxic gene as well as to evaluate the therapeutic effect. In this project, we will develop a nuclear gene imaging approach to assist the cytotoxic gene ... |
|
| Mechanism of Transcriptional Regulation by Androgen Receptor and its Coactivators in the Context of Chromatin |
JAN 2004 |
62 pages |
| Authors:
Jiemin Wong; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| Androgens play important roles in the differentiation, development and maintenance of male reproductive functions, as well as in the etiology of prostate cancer. The biological effects of androgens are believed to be mediated through the intracellular androgen receptor (AR), which belongs to the nuclear receptor (NR) superfamily of ligand-regulated transcription factors. Like other NRs, the actions of AR are subject to modulation, either positively or negatively, by an increasing number ... |
|
| Ubiquitin Pathway Enzymes: Coactivators of Nuclear Hormone Receptor and Their Role in the Development of Breast Cancer |
JAN 2004 |
58 pages |
| Authors:
Zafar Nawaz; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| Steroid hormones, estrogen, and progesterone, and their intracellular receptors play an important role in the development and progression of breast cancer. Coactivator proteins modulate the biological activity of these hormone receptor. We have cloned an E3 ubiquitin-protein ligase enzyme, E6-associated protein (E6-AP) and E2 ubiquitin- conjugating enzyme, UbcH7 as coactivators of steroid hormone receptors. The purpose of this research is to explore the possibility that the altered expression of E6-AP ... |
|
| Occupational Analysis Products: Diagnostic Imaging- AFSC 4R0X1 (CD-ROM) |
17 SEP 2003 |
|
| Authors:
Holly Hector; AIR FORCE OCCUPATIONAL MEASUREMENT SQUADRON RANDOLPH AFB TX
|
 | ELECTRONIC FILE CHARACTERISTICS: 18 files; HyperText Markup Language (.HTML), MS Word (.DOC), and MS PowerPoint (.PPT). PHYSICAL DESCRIPTION: 1 computer laser optical disc (CD-ROM); 4 3/4 in.; 51.2 MB. SYSTEMS DETAIL NOTE: IBM-clone PC-compatible. ABSTRACT: This is a report of an occupational survey of the Diagnostic Imaging career ladder, conducted by the Occupational Analysis Flight, AFOMS. The OSR reports the findings of current data that are available for use in ... |
|
| A Novel High Resolution Positron Emission Tomography System for Measurement of Bone Metabolism |
SEP 2003 |
66 pages |
| Authors:
John A. Correia; MASSACHUSETTS GENERAL HOSPITAL BOSTON
|
| The purpose of the work reported here is to develop high-resolution PET instrumentation for imaging the long bones. To-date a single plane prototype and a single plane second-generation instrumentation have been completed and evaluated. A generalized multi-planar detector element has also been designed and evaluated experimentally and a volumetric instrument has been designed through simulation studies. Radiopharmaceuticals for bone growth rate imaging have been developed for use in a demonstration ... |
|
| The Role of Steroid Receptor Coactivators in the Development of Prostate Cancer |
SEP 2003 |
16 pages |
| Authors:
Jang H. Cho; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| In prostate cancer, androgen receptor (AR) supervises several key genes expressions. In the cell. AR exerts its regulatory control on a target cell only in the presence of its ligand, androgen. The regulatory functions of AR are more complex and are fine-tuned by accessory proteins. These proteins are required for the maximum biological impact by androgen. These modulators, called coactivators, provide a positive stimulus for receptor action. Our laboratory has ... |
|
| Role of a Novel Splice Variant of the Steroid Receptor Coactivator AIB1 in Breast Cancer |
AUG 2003 |
44 pages |
| Authors:
Anna T. Riegel; GEORGETOWN UNIV WASHINGTON DC
|
| In this proposal we have investigated the hypothesis that overexpression of a novel truncated version of AIB1 (Delta-3AIB1) that we have found to be overexpressed in breast cancer is import for tumor development by impacting upon nuclear hormone receptor function. We have examined the hypothesis that the novel AIB1 variant has an altered function that changes its interaction with nuclear receptors such as the estrogen or progesterone receptor. We propose ... |
|
| Intraoperative Imaging of Sentinel Lymph Nodes |
AUG 2003 |
55 pages |
| Authors:
Carmen M. Greene; Nolan E. Hertel; GEORGIA TECH APPLIED RESEARCH CORP ATLANTA GA
|
| The main objective of this investigation is to determine the feasibility and value of the intraperative use of a small field of view (FOV) camera gamma camera for sentinel lymph node (SLN) localization in breast cancer patients. Two cameras, on with a 1' x 1' FOV and one with a 5 x 5' FOV are being investigated. Clinical experience with 14 subjects using the 1 in x 1 in FOV ... |
|
| Nuclear Imaging for Assessment of Prostate Cancer Gene Therapy |
APR 2003 |
6 pages |
| Authors:
Dongfeng Pan; VIRGINIA UNIV CHARLOTTESVILLE
|
| Combination of the cytotoxic viral thymidine kinase (tk) and the prodrug, acyclovir (ACV) has been reported to inhibit the growth of the C4-2 tumor, a subline of LNCaP. However, it remains unsolved to non-invasively detect the in vivo distribution, expression and persistence of the toxic gene as well as to evaluate the therapeutic effect. In this project, we will develop a nuclear gene imaging approach to assist the cytotoxic gene ... |
|
| Molecular Imaging for IMRI-Guided Minimally Invasive Treatment of Prostate Cancer |
MAR 2003 |
214 pages |
| Authors:
Baowei Fei; David L. Wilson; Jeffrey L. Duerk; D. B. Sodee; CASE WESTERN RESERVE UNIV CLEVELAND OH
|
| Our long-range objective is to develop an image-guided, minimally invasive thermal ablation treatment method for the prostate cancer. Before this method can he achieved, significant engineering developments are required. |
|
| 99M Tc-Peptides for Detection of Breast Cancer |
AUG 2002 |
27 pages |
| Authors:
Mary Rusckowski; MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER
|
| The goal of this project was to identify peptides from phage display peptide libraries which bind with high affinity to the mutant EGFRvIII receptor present in breast tumors. The peptides selected were radiolabeled with technetium-99m (99mTc) and tested for their potential as agents in the detection of breast cancer. Using available phage display peptide libraries, we have identified five consensus peptides that show affinity for cells expressing the mutant EGFRvIII ... |
|
| Role of a Novel Splice Variant of the Steroid Receptor Coactivator AIB1 in Breast Cancer |
AUG 2002 |
14 pages |
| Authors:
Anna T. Riegel; GEORGETOWN UNIV WASHINGTON DC
|
| In this proposal we have investigated the hypothesis that overexpression of a novel truncated version of AIB1 (Delta3AIBl) that we have found to be overexpressed in breast cancer is import for tumor development by impacting upon nuclear hormone receptor function. We have examined the hypothesis that the novel AIB1 variant has an altered function that changes its interaction with nuclear receptors such as the estrogen or progesterone receptor. We propose ... |
|
| Targeted Alpha Therapy Using Components of the Plasminogen Activation System for the Control of Micrometastatic Breast Cancer |
AUG 2002 |
|
| Authors:
Barry J. Allen; Marie Ranson; SAINT GEORGE HOSPITAL KOGARAH (AUSTRALIA) CANCER CARE CENTER
|
| We have developed a new adjuvant therapy for the control of metastatic breast cancer. This therapy exploits the involvement of cell-surface receptor bound urokinase plasminogen activator (uPA) in the metastatic spread of breast cancer cells. Once bound to specific cell-surface receptors, uPA efficiently activates plasminogen to the broad- spectrum protease, plasmin. We have successfully labeled and tested recombinant human PAI2 with the alpha radioisotope 213Bi to produce alpha-PAI2. Low doses ... |
|
| New Approaches in SPECT Breast Imaging |
JUL 2002 |
19 pages |
| Authors:
Brett Pieper; R. Jaszczak; M. Tornai; J. Bowsher; DUKE UNIV DURHAM NC
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| Parallel-beam Tilted-Head SPECT (TH-SPECT) was implemented on a SPECT system for its potential to image breast lesions and nearby axilla of seated, upright women. Breast, liver, and myocardial activity were included in order to simulate direct contamination and Compton scattering expected in clinical scans. Results indicated an increase in axial blurring with greater tilt angle. Reconstructions of combined fillable breast and torso phantoms containing two 1. 15 ml lesions were ... |
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NUCLEAR MEDICINE
