| Bioenergetic Approaches and Inflammation of MPTP Toxicity |
Sep-2009 |
13 pages |
| Authors:
M F Beal; CORNELL UNIV NEW YORK
|
 | This project was to develop novel therapies for Parkinson's Disease (PD), to test the effects of human dopaminergic stem cells, and to utilize metabolomic profiling to develop biomarkers for PD. We also developed a new transgenic mouse model of PD by knocking out the gene PINKI. Utilizing microdialysis we showed that PINK I deficient mice have impaired dopamine release. We demonstrated that rolipram, mitochondrial targeted antioxidant peptides, celastrol and promethazine ... |
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| SSMAS NMR Study of HD, GD, and VX on Carbon Fiber Textiles for Wipes |
01-Jul-2008 |
23 pages |
| Authors:
Carol A Brevett; Brian K MacIver; Kenneth B Sumpter; Dennis K Rohrbaugh; SCIENCE APPLICATIONS INTERNATIONAL CORP (SAIC) ABINGDON MD
|
| The C13 and P31 SSMAS NMR were used to study the degradation of HD, GD, and VX on a mesoporous and a microporous carbon fiber textile. The degradation of HD was faster than on ambient sand, concrete, limestone, and asphalt, and comparable to moist sand, concrete, limestone, and asphalt, forming toxic H-2TG and non-toxic TDG. The HD absorbed on both fiber textiles. The VX degradation rate on the carbon fiber ... |
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| Photochemical Degradation of Composition B and Its Components |
SEP 2007 |
64 pages |
| Authors:
Judith C. Pennington; Kevin A. Thorn; Larry G. Cox; Denise K. MacMillan; Sally Yost; Randy D. Laubscher; ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS ENVIRONMENTAL LAB
|
| Products of photodecomposition of 2,4,6-trinitrotoluene (TNT) have been observed as a coating on TNT particles and as a fine powdered residue surrounding TNT particles on ranges receiving limited rainfall. The significance of photolysis of explosive formulations on training ranges is unknown. Therefore, photolysis of a common explosive formulation, Composition B, and its components in a soil matrix were evaluated. Objectives included determination of photolysis rates, effects of light intensity and ... |
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| Effect of Treatment pH on the End Products of the Alkaline Hydrolysis of TNT and RDX |
JUN 2007 |
40 pages |
| Authors:
Jeffrey L. Davis; Catherine C. Nestler; Deborah R. Felt; Steven L. Larson; ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS ENVIRONMENTAL LAB
|
| The development of effective treatment options for munitions contaminants is essential for Department of Defense live fire range management and sustainability. The energetic compounds 2,4,6-trinitotoluene (TNT) and hexahydro-13,5-trinitro-1,3,5-triazine (RDX) have been identified in range soil. Alkaline hydrolysis, initiated through treatment with lime, has been shown to effectively destroy these compounds in solution and in well-mixed soil. This study was conducted to evaluate the effect of treatment pH on the end ... |
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| Investigation of Hair Follicle and Plasma Biomarkers for Low-Level VX Vapor Exposure |
01 NOV 2006 |
8 pages |
| Authors:
E. M. LaFiandra; A. E. Chambers; J. Horsmon; J. J. Valdes; J. W. Sekowski; B. Benton; M. Horsmon; R. Mioduszewski; S. Thomson; C. Evans; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
|
| Currently, there are no field-ready and expedient assays able to determine whether someone has been exposed to very low-level chemical warfare agent (CWA). Furthermore, if it were known that a person had been exposed, there is a paucity of information regarding the potential for transient or persistent neurological or other injuries. While our laboratory is working to address both of these problems, our recent work toward finding a more expedient ... |
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| High-Pressure Liquid Chromatograph with Mass Spectrometric Detection for Analysis of Supercritical Fuels Pyrolysis Products |
01 AUG 2006 |
52 pages |
| Authors:
Mary J. Wornat; Michelle L. Somers; Jennifer W. McClaine; Jorge O. Ona; LOUISIANA STATE UNIV BATON ROUGE DEPT OF CHEMICAL ENGINEERING
|
| A high-pressure liquid chromatograph with ultraviolet-visible diode-array detection and mass spectrometer (HPLC/UV/MS), purchased with DURIP funds, was used to analyze polycyclic aromatic hydrocarbons (PAH) produced in supercritical pyrolysis experiments with the model fuels 1-methylnaphthalene and toluene. The HPLC/UV/MS instrument facilitated the identification of fifteen 5- to 9-ring PAH from supercritical 1-methylnaphthalene pyrolysis and five 7- to 9-ring PAH from supercritical toluene pyrolysis none of which had ever before been identified ... |
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| An Electrochemiluminescence-Based Competitive Displacement Immunossay for the Type-2 Brevetoxius in Oyster Extracts |
24 JUL 2006 |
7 pages |
| Authors:
Mark A. Poli; Victor R. Rivera; Dwayne D. Neal; Daniel G. Baden; Shawn A. Messer; Steven M. Plakas; Robert W. Dickey; Kathleen E. Said; Leanne Flewelling; David Green; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80% methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100% acetone extracts, aqueous homogenates, and the clinical matrixes urine and serum can also be analyzed without significant matrix interferences. The assay offers the advantages of speed (2 h analysis time); ... |
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| Proteomic Analysis to Identify Novel Circulating Breast Cancer Markers |
JUN 2006 |
14 pages |
| Authors:
Francisco J. Esteva; M D ANDERSON CANCER CENTER HOUSTON TX
|
| Serum protein profiling using mass spectrometry is a promising approach to identify novel circulating breast cancer markers. In this study, serum was fractionated to deplete highly abundant proteins. After protein digestion, we used liquid chromatography mass spectrometry (LC-MS) to develop diagnostic fingerprints using bioinformatic techniques. Samples were randomized prior to fractionation and mass spectrometry testing. Each fraction was digested with trypsin and subsequently analyzed by LC-MS. Peptides were targeted based ... |
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| Identification of Sildenafil (Viagra) and Its Metabolite (UK 103,320) in Six Aviation Fatalities |
FEB 2006 |
14 pages |
| Authors:
Robert D. Johnson; Russell J. Lewis; FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST
|
| During the investigation of aviation accidents, postmortem samples from victims are submitted to the Federal Aviation Administration's Civil Aerospace Medical Institute for toxicological analysis. This report presents a rapid and reliable method for the identification and quantitation of sildenafil (Viagra ) and its active metabolite, UK-103,320. This procedure utilizes sildenafil-d8 as an internal standard for more accurate and reliable quantitation. The method incorporates solid-phase extraction and LC/MS/MS and MS/MS/MS utilizing ... |
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| Results of Analyses of the Fungicide Chlorothalonil, Its Degradation Products, and Other Selected Pesticides at 22 Surface-Water Sites in Five Southern States, 2003-04 |
2006 |
69 pages |
| Authors:
Elisabeth A. Scribner; James L. Orlando; William A. Battaglin; Mark W. Sandstrom; Kathryn M. Kuivila; Michael T. Meyer; GEOLOGICAL SURVEY RESTON VA
|
| In accordance with% the mission of the U. S. Geological} Survey (USGS) Toxic Substances Hydrology Program, a pesticide study was conducted during 2003-2004 to determine the occurrence of the fungicide chlorothalonil and its degradation products at 22 surface-water sites in the Southern States. Water-quality samples were collected during the peanut-growing season (June-September) in 2OO3. During the peanut growing season in 2004, samples were collected after large storms. An analytical method ... |
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| Verification of Single-Peptide Protein Identifications by the Application of Complementary Database Search Algorithms |
20 OCT 2005 |
29 pages |
| Authors:
James G. Rohrbough; Linda Breci; Nirav Merchant; Susan Miller; Paul A. Haynes; ARIZONA UNIV TUCSON DEPT OF BIOCHEMISTRY
|
| Protein identifications from complex biological mixtures often involve the application of tandem mass spectrometry techniques. One such technique, known as the Multi-Dimensional Protein Identification Technique, or MudPIT, involves the use of computer search algorithms that automate the process of identifying proteins present in the sample mixture based on mass spectrometry analysis. This technique involves digestion of the protein mixture with a protease such as trypsin, followed by liquid chromatography separation ... |
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| Detection and Identification of Bacteria Using Automated Biological Sample Processing With a Relational Database Management System |
01 OCT 2005 |
14 pages |
| Authors:
Rabih E. Jabbour; Samir V. Deshpande; A. P. Snyder; GEO-CENTERS INC ABERDEEN PROVING GROUND MD
|
| The biological sample processing system (BSPS) combines sample pretreatment, protein concentration, and separation modules and was interfaced to electrospray ionization mass spectrometry (ESI-MS). Multivariate analysis software capabilities were used for automated data processing. The bacterial identification by the BSPS-MS is based on Liquid chromatography-mass spectrometry analysis of various bacterial proteins followed by their correlation with in-house database constructed from several bacteria of interest using our in-house ProMAPDB software. The identification ... |
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| Mass Spectrometric Determination of Chemical Warfare Agents in Indoor Sample Media Typically Collected During Forensic Investigations |
OCT 2005 |
|
| Authors:
P. A. D'Agostino; J. R. Hancock; C. L. Chenier; DEFENCE RESEARCH AND DEVELOPMENT SUFFIELD (ALBERTA)
|
 | Terrorist use of chemical warfare agents against civilian targets could involve the targeting of enclosed populated spaces. DRDC Suffield, in collaboration with the Royal Canadian Mounted Police, identified a need for analytical methods for chemical warfare agent identification in media, including flooring, wall surfaces, office fabrics and paper products, that would typically be collected in an office environment during forensic investigations. Typical office environment media were spiked at the 4 ... |
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| Design and Testing for a Nontagged F1-V Fusion Protein as Vaccine Antigen Against Bubonic and Pneumonic Plague |
AUG 2005 |
23 pages |
| Authors:
Bradford S. Powell; Gerard P. Andrews; Jeffrey T. Enama; Scott Jendrek; Chris Bolt; Patricia Worsham; Jeffrey K. Pullen; Wilson Ribot; Harry Hines; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| A two-component recombinant fusion protein antigen was re-engineered and tested as a medical counter measure against the possible biological threat of aerosolized Yersinia pestis. The active component of the proposed subunit vaccine combines the F1 capsular protein and V virulence antigen of Y. pestis and improves upon the design of an earlier histidine-tagged fusion protein. In the current study, different production strains were screened for suitable expression and a purification ... |
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| Simultaneous Quantitation of Atenolol, Metoprolol, and Propranolol in Biological Matrices Via LC/MS |
MAY 2005 |
20 pages |
| Authors:
Robert D. Johnson; Russell J. Lewis; FEDERAL AVIATION ADMINISTRATION WASHINGTON DC OFFICE OF AVIATION MEDICINE
|
| Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol and propranolol - were among the 200 most prescribed medications in the United States in 2003. Pilots that successfully manage their hypertension can remain certified to fly. The Federal Aviation Administration currently designates ... |
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| Pre-Screening for Explosives Residues in Soil Prior to HPLC Analysis Utilizing Expray |
FEB 2005 |
19 pages |
| Authors:
Kevin L. Bjella; ENGINEER RESEARCH AND DEVELOPMENT CENTER HANOVER NH COLD REGIONS RESEARCH AND ENGINEERING LAB
|
| The characterization of Department of Defense (DoD) training lands for explosives residues involves sampling and analyzing soil samples collected at firing points and impact areas. Laboratory instruments used for this analysis are susceptible to accumulating high concentrations of these compounds, thus possibly requiring reanalyzing. By using the Expray Explosives Detection Kit to determine the approximate concentration of explosives compounds prior to analysis, soil extracts can be diluted to a concentration ... |
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| Identification, Cloning, and Expression of Potential Diagnostic Markers for Q Fever |
2005 |
4 pages |
| Authors:
C. C. Chao; H. W. Chen; X. Li; W. B. Xu; B. Hanson; W. M. Ching; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
|
| The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, ... |
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| Proteomic Analysis of Rickettsia prowazekii |
2005 |
4 pages |
| Authors:
C. C. Chao; D. Chelius; T. Zhang; E. Mutumanje; W. M. Ching; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
|
| Rickettsia prowazekii is an obligate intracellular gram-negative bacterium. Comparative proteomics study of a virulent strain (Breinl) versus an avirulent strain (Madrid E) was performed using an integrated liquid chromatography and mass spectrometer. About 30% of predicted proteins were detected and identified. Among the detected proteins, more than 30 proteins were of unknown function in both strains. Although several proteins were detected in only one strain, the overall distribution of detected ... |
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| GC-MS LC-MS and LC-MS/MS of Soil Samples from a Former Mustard Storage Site for the Presence of Mustard Thiodiglycol and Related Compounds |
JUL 2004 |
|
| Authors:
P. A. D'Agostino; J. R. Hancock; C. L. Chenier; DEFENCE RESEARCH AND DEVELOPMENT SUFFIELD (ALBERTA)
|
| Soil samples were taken for the first time at a number of locations and depths near a former mustard storage site as part of an environmental assessment. The contactor doing the sampling selected a number of soil samples from the site for mass spectrometric analysis. Mustard was not detected in the dichloromethane extracts of the soil samples during capillary column GC-MS. However, three of the soil sample extracts were found ... |
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| Investigation of Soman Adducts of Human Hemoglobin by Liquid Chromatography |
APR 2004 |
11 pages |
| Authors:
B. A. Logue; B. J. Pieper; S. D. Royster-Cunningham; ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
|
| Verification of exposure to chemical agents is a requirement of the intelligence, depot, and demilitarization communities to confirm claims of exposure to chemical warfare agents. Improving current analytical methods and developing novel methods to verify chemical agent exposure are vital, considering the increased threat of chemical agent use and therefore the possibility of large numbers and types of biomedical samples that a relatively small number of labs must analyze. The ... |
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| Quantifying the Effects of Preventive Foods on the Metabolism of a Prostate Carcinogen in Humans and in Prostate Cells Grown in Culture |
APR 2004 |
81 pages |
| Authors:
James S. Felton; LAWRENCE LIVERMORE NATIONAL LAB CA
|
| We are investigating the effects of foods associated with reduced prostate cancer risk on a cooked meat carcinogen known to be associated with elevated cancer risk. Cooked muscle meats contain potent mutagens and carcinogens belonging to the heterocyclic amine class of compounds. One of these, PhIP, is a genotoxic carcinogen that has been shown to cause DNA damage in prostate tissue and prostate tumor formation in rats. We have developed ... |
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| Comparison of LC-ESI-MS and GC-MS for the Analysis of a Synthetic Tabun Sample |
DEC 2003 |
|
| Authors:
Paul A. D'Agostino; J. R. Hancock; C. L. Chenier; DEFENCE RESEARCH AND DEVELOPMENT SUFFIELD (ALBERTA)
|
| Packed capillary LC-ESI-MS and capillary column GC-MS were compared for the analysis of a synthetic tabun sample as each method has advantages for the analysis of samples containing chemical warfare agents, their hydrolysis products and related compounds. Twelve sample components were identified during LC-ESI-MS analysis of the sample, while only ten were detected by GC-MS. The two less volatile compounds not detected by GC-MS contained hydroxyl substitution and would only ... |
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| Ethanol Origin in Postmortem Urine: An LC/MS Determination of Serotonin Metabolites |
NOV 2003 |
18 pages |
| Authors:
Robert D. Johnson; Russell J. Lewis; Dennis V. Canfield; C. L. Blank; FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST
|
| Specimens from fatal aviation accident victims are submitted to the FAA Civil Aerospace Medical Institute for toxicological analysis. During toxicological evaluations, ethanol analysis is performed on all cases. Care must be taken when interpreting a positive ethanol result due to the potential for postmortem ethanol formation. Several indicators of postmortem ethanol formation exist; however, none are completely reliable. The consumption of ethanol has been shown to alter the concentration of ... |
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| WRAIR Protocols for Soldier Status and Readiness to Organophosphate Exposure: Unprocessed Whole Blood Cholinesterase and Pyridostigmine Bromide Quantification |
01 JUL 2003 |
5 pages |
| Authors:
Gregory E. Garcia; Shawn R. Feaster; Deborah R. Moorad; Bhupendra P. Doctor; Richard K. Gordon; Connie R. Clark; J. R. Smith; Brian J. Lukey; Raven E. Reitstetter; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD
|
| Exposure to nerve agents, OPs, pesticides, anesthetics, terrorists' chemical agents, and drugs of abuse such as cocaine, heroin, and some neuro-degenerative disease states selectively reduces AChE and/or BChE activity. Since urban terrorism is on the rise, Federal, State, and local authorities need a reliable, fast, inexpensive method for confirming such an assault to initiate appropriate containment, decontamination, and treatment measures. Thus, we developed a semi-automated medical diagnostic microplate procedure capable ... |
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| Application of Cavity Ring-Down Spectroscopy to Liquid Samples |
15 MAY 2003 |
7 pages |
| Authors:
Richard N. Zare; STANFORD UNIV CA DEPT OF CHEMISTRY
|
| Three main strategies in the application of CRDS to liquids have been investigated. In the direct liquid approach, an enclosed cavity is entirely filled with liquid for the purpose of quantifying low concentrations of solutes in a bulk solution and monitoring reactions in solution. A picomolar detection limit for a strongly absorbing solute in acetonitrile has been demonstrated, and interesting kinetic behavior has been observed at low concentrations. In the ... |
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| Neurotoxicity From Chronic Exposure to Depleted Uranium |
APR 2003 |
9 pages |
| Authors:
Stephen M. Lasley; ILLINOIS UNIV AT CHICAGO
|
| This project is designed to test the hypothesis that chronic exposure to depleted uranium (DU) impairs neuronal processes underlying cognitive function via alterations induced at hippocampal glutamatergic synapses. As prescribed by the Statement of Work, efforts were initiated in year I on Technical Objectives 1 (establishment of chronic exposure protocol) and 2 (defining integrity of hippocampal glutamate release) and are continuing into year 2. Blood and brain tissue are being ... |
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| Analysis of Human AXIN2 and Other Wnt Signal Pathway Genes in Human Prostate Cancer |
MAR 2003 |
12 pages |
| Authors:
Xianshu Wang; MAYO CLINIC ROCHESTER MN
|
| Upregulation of cyclin D1, a key regulator of cell cycle, by elevated beta-catenin through the disruption of Wnt signaling pathway is often seen in colon cancer and breast cancer. Cyclin D1 overexpression is also reported in about 25% of human prostate cancer where only about 5% mutation in beta catenin is found. Human AXIN2 and other components in the Wnt signaling pathway may also contribute to cyclin D1 elevation. |
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| Characterization of the Structural and Chemical Properties of Copper Chelators in Marine Systems |
FEB 2003 |
|
| Authors:
James W. Moffett; Nelson M. Frew; WOODS HOLE OCEANOGRAPHIC INST MA DEPT OF MARINE CHEMISTRY AND GEOCHEMISTRY
|
| Copper speciation in seawater is generally dominated by low concentrations of strong, highly selective Cu chelators At present, we have no data to account for the high specificity and high binding affinity of these compounds for Cu, nor can we validate hypotheses regarding their sources and sinks. We propose to study the structural and chemical properties of strong Cu chelators produced by a ubiquitous marine cyanobacterium, Synechococcus sp., a plausible ... |
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| Analysis of Altered Protein Components of Interchromatin Granule Clusters from Breast Cancer Cells |
FEB 2003 |
20 pages |
| Authors:
Prasanth K. Kannanganattu; COLD SPRING HARBOR LAB NY
|
| Numerous studies have identified a variety of morphological changes in the cell nuclei that can be correlated with cancer. We have been interested in the function of a specific nuclear compartment, interchromatin granule clusters (TGCs), and in determining if changes in its protein composition occur in breast cancer cells. We have previously identified 138 proteins from mouse ICGs which includes proteins involved in pre-mRNA processing. Using this as basis, we ... |
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| Production and Purification of Refolded Recombinant Human IL-7 From Inclusion Bodies |
2003 |
|
| Authors:
Thomas Ouelette; Sophie Destrau; Timothy Ouelette; Jianwei Zhu; John M. Roach; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| A recombinant clone of human rhIL-72 that codes for 152 amino acids was cloned into Escherichia coli HMS 174(DE3) pLys S under the control of a T7 promoter. The cloned rhIL-7 has a molecular weight of 17.4 kDa with three disulfide bonds and three sites for glycosylation. The molecule has a hydrophobic nature and heparin affinity. The isoelectric point of this recombinant protein is 9.3 by CZE and 8.45 by ... |
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| Dissolution Kinetics of High Explosive Compounds (TNT, RDX, HMX) |
SEP 2002 |
133 pages |
| Authors:
Jason C. Lynch; ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS ENVIRONMENTAL LAB
|
| The production and usage of high explosive compounds such as 2,4,6- trinitrotoluene (TNT), hexahydro-l,3,5-trinitro-1,3,5-triazine (RDX), and octahydro-I,3,5,7-tetranitro-1,3,S,7-tetrazocine (HMX) have resulted in their release in the environment. These compounds pose potential harm because of their reactivity and effects on health. Agencies that use high explosive compounds are working to better understand the physical and chemical properties surrounding these explosive compounds to include their fate and transport, effects on health, and remediation ... |
|
| Genetics of Breast Cancer in Blacks |
SEP 2002 |
5 pages |
| Authors:
Olufunmilayo I. Olopade; CHICAGO UNIV IL
|
| Breast cancer in these young Black women is more virulent, leading to a decrease in the overall survival rates for - -- African Americans diagnosed with breast cancer when compared to Whites. Our studies involve a new approach that involves large numbers of breast cancer patients from Africa and provide the first concerted effort to seriously address the contribution of genetic risk factors to the high incidence and mortality from ... |
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| Process Development for the Production of a Monoclonal Antibody Against Francisella Tularensis |
JUN 2002 |
28 pages |
| Authors:
Jun T. Park; Sarah J. Cork; Tracy Collano; CHeng J. Cao; Derrel Menking; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
|
| Antibodies are essential components in biosensors and enable the detection of biological warfare agents with both sensitivity and selectivity. A biomanufacturing process was developed for the production of a monoclonal antibody (mAb) specific for detecting the Frencisella tularensls organism in the environment. This report describes a process for the production of 12 g of monocional antibody from an in vitro call culture system. Hybridoma calls were grown in 2-L gas ... |
|
| Quantifying the Effects of Preventive Food on the Metabolism of a Prostate Carcinogen in Humans and in Prostate Cells Grown in Culture |
APR 2002 |
56 pages |
| Authors:
James S. Felton; LAWRENCE LIVERMORE NATIONAL LAB CA
|
| We are investigating the effects of foods associated with reduced prostate cancer risk on a dietary carcinogen known to be associated with cooked meat and elevated cancer risk. Cooked muscle meats contain potent mutagens and carcinogens belonging to the heterocyclic amine class of compounds. One of these, PhIP, is a genotoxic carcinogen that has been shown to cause DNA damage in prostate tissue and prostate tumor formation in rats. We ... |
|
| Cationic Polymerization (Cure Kinetics) of Model Epoxide Systems |
APR 2002 |
52 pages |
| Authors:
Reza Dabestani; Ilia N. Ivanov; James M. Sands; ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD
|
| Cationic polymerization of epoxy resins can be induced by ultraviolet (UV) or electron beam (E-beam) radiation and proceeds very efficiently in the presence of an appropriate photoinitiator. Although good thermal properties have been obtained for some E-beam cured epoxy resins, other important mechanical properties, such as interlaminar shear strength, fracture toughness, and compression are poor and do not meet aerospace manufacturers materials standards. We have initiated a comprehensive study to ... |
|
| Analysis of Nitroglycerine in Soils and on Mortar Fins Using GC-TID |
FEB 2002 |
25 pages |
| Authors:
Alan D. Hewitt; ENGINEER RESEARCH AND DEVELOPMENT CENTER HANOVER NH COLD REGIONS RESEARCH AND ENGINEERING LAB
|
| A method of analysis for nitroglycerine (NG) in soil and on the surfaces of mortar fins (a common range scrap material) was developed using a field-portable gas chromatograph system. The method combines quick and simple sample preparation procedures with a rapid gas chromatographic (GC) analysis using a thermionic ionization detector (TID) that is selective for compounds containing nitro (NO2) functional groups. Very good agreement was observed among NG values established ... |
|
| Analysis of Altered Protein Components of Interchromatin Granule Clusters from Breast Cancer Cells |
FEB 2002 |
11 pages |
| Authors:
Prasanth K. Kannanganattu; COLD SPRINGS HARBOR LAB NY
|
| Intimate links between the morphology changes of some of sub-nuclear structures and diseases such as cancer have been shown. Thus far, a number of pre-mRNA splicing factors, RNA polymerase II and kinases as well as several cancer related proteins have been reported to be localized to the IGCs. The present study sets a precedent for the global identification of potential differences in the protein composition of IGCs from breast cancer ... |
|
| Microelectromechanical Systems (MEMS)-Based Microcapillary Pumped Loop for Chip-Level Temperature Control |
JAN 2002 |
108 pages |
| Authors:
Jeffrey A. Kirshberg; CALIFORNIA UNIV BERKELEY
|
| Utilizing current microelectromechanical systems (MEMS) technologies, a three-port microcapillary pumped loop (micro-CPL) was designed, fabricated, and tested to provide integral cooling to electronics of MEMS-type devices. The two-wafer design consists of one silicon and one borofloat glass wafer. An analytical study, adopted from traditional CLP theory, was used in determining the geometry of the device, including the evaporator dimensions (1000 by 2000 micrometers) and the length of the liquid and ... |
|
| Genetics of Breast Cancer in Blacks |
SEP 2001 |
5 pages |
| Authors:
Olufunmilayo I. Olopade; CHICAGO UNIV IL
|
| Breast cancer in young Black women is more virulent, leading to a decrease in the overall survival rates for African Americans diagnosed with breast cancer when compared to Whites. Our studies provide the first concerted effort to seriously address the contribution of genetic risk factors to the high incidence and mortality from breast cancer in young Black women. We have developed an efficient mechanism to recruit incident cases of early ... |
|
| Analysis of CL-20 in Environmental Matrices: Water and Soil |
AUG 2001 |
28 pages |
| Authors:
Steven L. Larson; Deborah R. Felt; Lynn Escalon; Jeffrey D. Davis; Lance D. Hansen; CORPS OF ENGINEERS WASHINGTON DC
|
| The determination of new explosive formulations in environmental matrices is necessary for understanding both the environmental threat these compounds present and the effectiveness of remediation technologies for the treatment of contaminated soils and waters. Analytical techniques for the detection of 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazatetracyclo5 .5.0.05,9. 03,11dodecane (CL-20) in water and soil have been developed. Methods traditionally utilized for the analysis of nitroaromatics were adapted for the determination of CL-20, a cage-like explosive compound. ... |
|
| Role of the ATM Gene in Bilateral Breast Cancer Following Radiotherapy |
AUG 2001 |
97 pages |
| Authors:
Barry Rosenstein; MOUNT SINAI SCHOOL OF MEDICINE NEW YORK
|
| The purpose of this project was to screen women who developed either unilateral or bilateral breast cancer for mutations and polymorphisms in the ATM gene. To accomplish this, during the first three years of this project, DNA samples were isolated from breast cancer patients and screened using a Non- radioisotopic RNase Cleavage- based Assay (NIRCA). However, not a single mutation was discovered from any of these patients which raised a ... |
|
| Fuels Combustion Research: Supercritical Fuel Pyrolysis |
19 JUN 2001 |
4 pages |
| Authors:
Mary J. Wornat; PRINCETON UNIV NJ DEPT OF MECHANICAL AND AEROSPACE ENGINEERING
|
| The fuels used in the next generation of hypersonic aircraft will have to operate under very high pressures (beyond the critical pressures of most hydrocarbons) and will have to sustain very high heat loads in order to meet aircraft cooling requirements, Potential candidates for meeting these stringent cooling requirements are 'endothermic' fuels, fuels that can undergo a controlled heat-absorbing chemical reaction (e.g., dehydrogenation) prior to combustion. Critical to the development ... |
|
| Organoarsenic Compounds II. Separation and Stereochemical Determination of 5-Substituted 2-Phenyl-1,3,2-Dithiarsenanes |
MAY 2001 |
|
| Authors:
Paul C. Bossie; Fu-Lian Hsu; George W. Wagner; Dennis K. Rohrbaugh; EDGEWOOD RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUNDMD
|
| The cis- and trans-isomers of 5-substituted 2-phenyl-1,3,2- dithiarsenanes (compounds 5 and 6) are the adducts of the lewisite simulant, phenyl dichloroarsine (3), and the 2-substituted 1,3-propanedithiols (1 and 2). The isomers were separated by reverse-phase liquid chromatography and identified by photodiode-array/mass spectrometry. The stereochemistry of these adducts is assigned based on H1 NMR Nuclear Overhauser Effect difference measurements. |
|
| Field Gas Chromatography/Thermionic Detector System for On-Site Determination of Explosives in Soils |
MAY 2001 |
29 pages |
| Authors:
Alan D. Hewitt; Thomas F. Jenkins; Thomas A. Ranney; COLD REGIONS RESEARCH AND ENGINEERING LAB HANOVER NH
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| On-site determination of nitroaromatic,nitramine,and nitrate ester explosives compounds in soils was performed using a field-portable gas chromatograph (GC)equipped with a thermionic ionization detector (TID)selective for compounds with nitro functional groups. Soil samples were extracted with acetone. A 1 microliter volume of the filtered soil extract was manually injected into the GC,allowing for the rapid qualification and quantification of the suite of explosives that often coexist in soils at military training ... |
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| Surface Phenomena of Solid Propellants Ignited by Plasma |
MAY 2001 |
43 pages |
| Authors:
Pamela J. Kaste; Amy E. Kinkennon; Robert J. Lieb; Avi Birk; Miguel Del Guercio; ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD
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| The morphological and chemical characterization of M30 propellants recovered after conventional and plasma ignition with polyethylene (PE) terephthalate (Mylar) in an interrupted closed bomb (extinguished at pressures between 35 and 100 Mpa) has been performed. It has been reported that burning rate augmentation appears to occur during the plasma event, but there was no evidence for post-plasma augmentation. For the extinguished grains from PE plasma ignition, there appears to be ... |
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| Structural Characterization of Lipopeptides Isolated from Bacillus Globigii Spores |
2001 |
66 pages |
| Authors:
Bruce H. Williams; MARYLAND UNIV COLLEGE PARK DEPT OF CHEMISTRY AND BIOCHEMISTRY
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| Several species of Bacillus spores produce surface-active antibiotic and antifungal lipopeptides, phospholipids, and polypeptides. Some of these are detected as biomarkers when intact spores are analyzed by Matrix Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry. Bacillus globigil spores, grown in new sporulation media (NSM), were suspended and then analyzed using a MALDI-TOF mass spectrometer to screen for biomarkers with 4- methoxycinnamic acid as matrix. Spores, vegetative cells, and the culture ... |
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| Fundamentals and Applications of Atmospheric Pressure Chemical Ionization Quadrupole Ion Trap Mass Spectrometry for the Analysis of Explosives |
2001 |
214 pages |
| Authors:
Richard F. Reich Jr.; FLORIDA UNIV GAINESVILLE DEPT OF CHEMISTRY
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| The goal of this research was to develop a liquid chromatography (LC) /atmospheric pressure chemical ionization (APCI)-mass spectrometry (MS) and tandem mass spectrometry (MS/MS) method for the analysis of explosives. APCI and electrospray ionization (ESI) are soft ionization techniques which produce characteristic ions of explosives at atmospheric pressure. Most explosives are susceptible to impurities in the solvent and form adduct ions instead of molecular-type ions. Additives allow specific adduct ions ... |
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| Electrochemical Reduction of Energetically Contaminated Wastewater: Development and Testing of Pilot Scale Reactor |
NOV 2000 |
33 pages |
| Authors:
Rajesh Doppalapudl; Dinesh Palaniswarmy; Stephen W. Maloney; George Sorial; ARMY OPERATIONS SUPPORT COMMAND ROCK ISLAND IL
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| TNT, DNT, and RDX are major constituents of munitions production wastewaters. DNT enters waste-streams during propellant production, whereas TNT and RDX enter waste-streams during munitions loading and demilitarization. This study evaluated the rate kinetics of electrochemical reduction of simulated wastewater containing similar organic strength as found in the Army munitions wastewaters. Experiments to study the electrochemical reduction of DNT in the presence of ethanol and a mixture of TNT and ... |
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| Electrospray LC/MS Detection of EA 2192 in Aqueous Solution |
AUG 2000 |
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| Authors:
Dennis K. Rohrbaugh; Michael W. Elizy; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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| This report describes efforts to detect EA 2192, S-2- (DIISOPROPYLAMINO)ETHYL methylphosphonothioic acid, in aqueous solutions below the drinking water standard level of 20 ppb. LC/MS electrospray ionization and tandem MS detection using a parent ion of 240 m/z and a product ion of 128 m/z were applied to obtain the desired analytical sensitivity, selectivity, and reproducibility. From replicate analyses of a 10-ppb standard, a ... |
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| Dose Uniformity of Over-the-Counter Melatonin as Determined by High- Pressure Liquid Chromatography |
AUG 2000 |
13 pages |
| Authors:
B. F. Prazinko; J. A. Sam; J. L. Caldwell; A. T. Townsend; ARMY AEROMEDICAL RESEARCH LAB FORT RUCKER AL
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| The purpose of this study was to determine the dose uniformity among various brands of over-the-counter (OTC) melatonin tablets (or capsules). Melatonin is a substance that may be used by Army aviation personnel, so education in regards to the actual amounts of melatonin contained in each tablet will be beneficial. High-pressure liquid chromatography was used to analyze eight different brands of tablets. From each brand, two ... |
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