| Adoptive Immunotherapy Combined with Hematopoietic Cell Transplantation as a Therapeutic Treatment of Prostate Cancer |
Jul-2009 |
19 pages |
| Authors:
Beatrice Knudsen; Scott S Graves; Rainer Storb; Bradley Stone; FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA
|
 | We determined that a prostate cell lysate prepared from canine prostate tissue was immunogenic when injected in female dogs. In addition to the known prostate antigen, canine prostate specific esterase (CPSE), we identified by molecular weight several other proteins against which the dog made IgG antibodies. Using a mixed lymphocyte reaction, we found that a cellular response was generated against the prostate cell lysate after the female dog was injected ... |
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| CD24 as a Potential Therapeutic Target in Prostate Cancer |
Apr-2009 |
28 pages |
| Authors:
Pan Zheng; MICHIGAN UNIV ANN ARBOR
|
| This is the first annual report on the grant CD24 as a Potential Therapeutic Target in Prostate Cancer. CD24 (heat-stable antigen) is a cell surface GPI-anchored mucin-like glycoprotein with broad expression on a variety of cell types, including hematopoietic cells, neuronal cells and various epithelial cells. There are accumulating evidence showing CD24 plays an important role in tumor development and tumor metastasis. We hypothesized that the expression of CD24 on ... |
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| Defining the Recruitment of Reactive Stroma Progenitor Cells to the Tumor Microenvironment of Human Prostate Cancer |
Feb-2009 |
20 pages |
| Authors:
David Barron; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| The reactive stroma microenvironment is important to prostate cancer, however specific mechanisms of its role in regulating prostate cancer progression are poorly understood. Human lung, breast, and colon carcinomas all exhibit alterations in the stromal compartment, yet the specific composition, the origin of reactive cells, and the factors that regulate this evolution are understudied. The myofibroblast appears to be a key component of carcinoma associated reactive stroma. This highly synthetic ... |
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| Adoptive Immunotherapy Combined with Hematopoietic Cell Transplantation as a Therapeutic Approach to Treatment of Prostate Cancer |
Oct-2008 |
14 pages |
| Authors:
Beatrice Knudsen; Scott S Graves; Rainer Storb; Bradley Stone; FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA
|
| We determined that cell lysates prepared from canine prostate tissue was immunogenic when injected in female dogs. In addition to the known prostate antigen, canine prostate specific esterase (CPSE), we identified by molecular weight several other proteins against which the dog made IgG antibodies. Using a mixed lymphocyte reaction, we found that a cellular response was generated against the prostate cell lysate after the female dog was injected 3 times ... |
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| Early Diagnosis, Treatment and Care of Cancer Patients |
Sep-2008 |
6 pages |
| Authors:
Richard Fisher; ROCHESTER UNIV NY
|
| This grant program encompasses two complimentary projects. The hypothesis that leukemia can be treated effectively by inhibition of putative cancer stem cells will be tested in project #1. This will be done by application of inhibitors of stem cells as a novel approach for eradication of leukemia tumor cells. Parthenolide (PTL)-based drugs and related drugs that inhibit nuclear factor kappa B (NF-kB) will be used. The effects of these drugs ... |
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| Characterization of the Hematopoietic Stem Cell in the Peripheral Blood of Patients with Idiopathic Myelofirosis |
Jun-2008 |
6 pages |
| Authors:
Ronald Hoffman; MOUNT SINAI SCHOOL OF MEDICINE NEW YORK
|
| The clinical course of patients with Philadelphia chromosome negative myeloproliferative disorder (MPD) is frequently complicated by thrombotic events. Post-natal vasculogenesis has been proposed to play a critical role in angiogenesis by acting through a hierarchy of endothelial progenitor cells (EPC). Some EPC have been shown to share a number of features associated with monocytes while other more primitive progenitor cells produce EC in vitro exclusively. The cells which share features ... |
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| Quarterly Performance/Technical Report of the National Marrow Donor Program |
05 FEB 2008 |
23 pages |
| Authors:
Michelle Setterholm; NATIONAL MARROW DONOR PROGRAM MINNEAPOLIS MN
|
| 1. CONTINGENCY PREPAREDNESS: Collect information from transplant centers, build awareness of the Transplant Center Contingency Planning Committee and educate the transplant community about the critical importance of establishing a nationwide contingency response plan. 2. RAPID IDENTIFICATION OF MATCHED DONORS: Increase operational efficiencies that accelerate the search process and increase patient access. These are key to preparedness in a contingency event. 3. IMMUNOGENETIC STUDIES: Increase understanding of the immunologic factors important ... |
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| Protein ISG15 Modification in the Development and the Treatment of Chronic Myeloid Leukemia |
JUN 2007 |
81 pages |
| Authors:
Dong-Er Zhang; SCRIPPS RESEARCH INST LA JOLLA CA
|
| Interferons are useful drugs in treating chronic myeloid leukemia (CML). One of the cellular responses of interferon treatment is the activation of protein modification by ISG15. We have cloned a novel gene encoding a protease UBP43 that specifically removes ISG15 from ISG15 modified proteins. Furthermore, we have generated UBP43 knockout mice. UBP43 deficient hematopoietic cells have much higher levels of ISG15 modified proteins upon interferon stimulation and are hypersensitive to ... |
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| DNA Methylation as an Epigenetic Factor in the Development and Progression of Polycythemia Vera |
OCT 2006 |
25 pages |
| Authors:
Jean-Pierre Issa; Jaroslav Jelinek; M D ANDERSON CANCER CENTER HOUSTON TX
|
| Polycythemia vera (PV) is a clonal myeloproliferative disorder (MPD) affecting erythroid, myelomonocytic, and megakaryocytic lineages. An activating somatic mutation of JAK2 tyrosine kinase is present in the majority of PV patients but also in 50% of patients with essential thrombocythemia (ET) and myelofibrosis (MF). Additonal factors are presumed to affect the phenotype and progression of the disease. We studied DNA methylation as a possible epigenetic factor in the development and ... |
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| Beta-catenin: A Potential Survival Marker of Breast Cancer Stem Cells |
SEP 2006 |
112 pages |
| Authors:
Jeffrey M. Rosen; Mercy S. Chen; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| The Wnt/ -catenin pathway has been extensively studied for its role in development and cancer. The canonical Wnt signal is transduced by -catenin, which acts as a transcriptional coactivator by associating with the Tcf/LEF family of transcription factors. It has been established that Wnt signaling regulates the self-renewal of normal stem cells in both the hematopoietic systems and the epidermis. In addition, constitutive activation of the Wnt pathway has been ... |
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| Sulfur Mustard-induced Neutropenia: Treatment with Granulocyte Colony-stimulating Factor |
2006 |
7 pages |
| Authors:
Dana R. Anderson; Wesley W. Holmes; Robyn B. Lee; Stephen J. Dalal; Charles G. Hurst; Beverly I. Maliner; Jonathan Newmark; William J. Smith; ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
|
| Although best known as a blistering agent, sulfur mustard (HD) can also induce neutropenia in exposed individuals, increasing their susceptibility to infection. Granulocyte colony- stimulating factor (G-CSF) and pegylated G-CSF (peg-G-CSF) have been approved by the U.S. Food and Drug Administration as hematopoietic growth factors to treat chemotherapy-induced neutropenia. The goal of this study was to determine the effectiveness of G-CSF and peg-G-CSF in ameliorating HD-induced neutropenia. African green monkeys ... |
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| Beta-Catenin: A Potential Survival Marker of Breast Cancer Stem Cells |
SEP 2005 |
9 pages |
| Authors:
Mercy S. Chen; Jeffrey M. Rosen; BAYLOR COLL OF MEDICINE HOUSTON TX
|
| The Wnt/beta-catenin pathway has been extensively studied for its role in development and cancer. It has been established that Wnt/beta-catenin signaling regulates the self renewal of normal stem cells in the hematopoietic system, the epidermis, as well as many other organs, but the importance of this pathway in breast stem/progenitors has not yet been investigated. The overall objective of this study is to determine the mechanisms by which beta-catenin might ... |
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| Protein IsG15 Modification in the Development and the Treatment of Chronic Myeloid Leukemia |
JUN 2005 |
20 pages |
| Authors:
Dong-Er Zhang; SCRIPPS RESEARCH INST LA JOLLA CA
|
| Interferons are useful drugs in treating chronic myeloid leukemia (CML). One of the cellular responses of interferon treatment is the activation of protein modification by ISO 15. We have cloned a novel gene encoding a protease UBP43 that specifically removes 15015 from 15015 modified proteins. Furthermore, we have generated UBP43 knockout mice. UBP43 deficient hematopoietic cells have much higher levels of ISO 15 modified proteins upon interferon stimulation and are ... |
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| Bone Marrow Function in Development of Childhood Asthma |
APR 2005 |
18 pages |
| Authors:
Mary B. Hogan; WEST VIRGINIA UNIV MORGANTOWN
|
| Asthma is the most common reason for hospitalization of children in both military and civilian hospitals. In children with asthma, pulmonary exposure to allergen results in damage to bronchioles by invasion of eosinophils. Eosinophils are inflammatory cells, have limited life spans, and must be continually renewed from hematopoietic tissue. We adapted an animal model of asthma to our laboratory for studies of the effect of pulmonary allergen exposure on eosinophil ... |
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| Evaluation and Development of Radiation Countermeasures at AFRRI |
2005 |
11 pages |
| Authors:
V. Srinivasan; K. S. Kumar; M. R. Landauer; M. B. Grace; A. C. Miller; V. K. Singh; T. M. Seed; C. M. Chang; C. E. Inal; ARMED FORCES RADIOBIOLOGY RESEARCH INSTBETHESDA MD
|
| Leukopenia after ionizing radiation is due largely to free radical injury to stem cells and progenitors in hematopoietic tissue. Recovery depends on the ability of the remaining stem cells and progenitors to proliferate and differentiate sufficiently to reconstitute the immune system before a lethal infection takes hold. Proliferation and differentiation of hematopoietic progenitor cells (HPC) are dependent on factors produced by cells in the hematopoietic microenvironment or niche. We recently ... |
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| Medical Managment of the Acute Radiation Syndrome: Recommendations of the Strategic National Stockpile Radiation Working Group |
15 JUN 2004 |
20 pages |
| Authors:
Jamie K. Waselenka; Thomas J. MacVittie; William F. Blakely; Nicki Pesik; Albert L. Wiley; William E. Dickerson; Horace Tsu; Dennis L. Confer; Norman Coleman; Thomas Seed; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
|
| Physicians, hospitals, and other health care facilities will assume the responsibility for aiding individuals injured by a terrorist act involving radioactive material. Scenarios have been developed for such acts that include a range of exposures resulting in few to many casualties. This consensus document was developed by the Strategic National Stockpile Radiation Working Group to provide a framework for physicians in internal medicine and the medical subspecialties to evaluate and ... |
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| Role of the SHP2 Phosphatase in Myeloid Growth Control and Chronic Myeloid Leukemia |
APR 2004 |
36 pages |
| Authors:
Kevin Shannon; CALIFORNIA UNIV SAN FRANCISCO
|
| This final report presents research data from studies performed during the initial 9 month period of a 3 year award from the U.S. Army CML research program. In the fall of 2003, the investigators were notified that an RO1 proposal on the same subject was approved for funding by the National Cancer Institute. After consultation with Army CML program personnel, we voluntarily relinquished this award so that the unused funds ... |
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| Orally Administered IL-6 Induces Elevated Intestinal GM-CSF Gene Expression and Splenic CFU-GM |
30 MAR 2004 |
7 pages |
| Authors:
F. M. Rollwagen; T. A. Davis; Y. Y. Li; N. D. Pacheco; X. L. Zhu; UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD
|
| Orally administered interleukin (IL)-6 has been shown to be of benefit in eliminating Campylobacter infection and in preventing sepsis following hemorrhage. In related experiments, it was seen that proliferating cells were found in the spleens of untreated mice given IL-6 by oral gavage. Injection of the DNA label, BrdU, showed that significant proliferation began at 4 h and peaked at 24 h in the splenic red pulp of animals given ... |
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| Bone Marrow Function in Development of Childhood Asthma |
MAR 2004 |
27 pages |
| Authors:
Mary B. Hogan; WEST VIRGINIA UNIV RESEARCH CORP MORGANTOWN*
|
| Asthma is the most common reason for hospitalization of children in both military and civilian hospitals. In children with asthma, pulmonary exposure to allergen results in damage to bronchioles by invasion of eosinophils. Eosinophils are inflammatory cells, have limited life spans, and must be continually renewed from hematopoietic tissue. We adapted an animal model of asthma to our laboratory for studies of the effect of pulmonary allergen exposure on eosinophil ... |
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| Antigen-Specific Immunotherapy Using Lentivirus-Transduced Hematopoietic Progenitor Cell: A Novel Approach for the Treatment of Metastatis Prostate Cancer |
JAN 2004 |
14 pages |
| Authors:
Linzhao Cheng; JOHNS HOPKINS UNIV BALTIMORE MD
|
| This group has completed the goal set for Task 1 ("To examine the immune responses to a model tumor antigen selectively expressed in DCs differentiated in vivo from transduced hematopoietic progenitor cells"). They have: 1) made lentiviral vectors expressing a model tumor antigen influenza hemagglutinin (HA); 2) developed a method to efficiently transduce mouse bone marrow progenitor cells that reconstitute immune and blood systems in irradiated and transplanted mice; 3) ... |
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| Bone Marrow Function in Development of Childhood Asthma |
MAR 2003 |
62 pages |
| Authors:
Mary B. Hogan; Kenneth S. Landreth; WEST VIRGINIA UNIV RESEARCH CORP MORGANTOWN*
|
| Asthma is the most common reason for hospitalization of children in both military and civilian hospitals. In children with asthma, pulmonary exposure to allergen results in damage to bronchioles by invasion of eosinophils. Eosinophils are inflammatory cells, have limited life spans, and must be continually renewed from hematopoietic tissue. We have adapted an animal model of asthma for studies of the effect of pulmonary allergen exposure on eosinophil progenitor cells ... |
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| Involvement of the Tyrosine Phosphatase SHP-1 in the Development of Breast Cancer |
OCT 2002 |
11 pages |
| Authors:
Ulrike Lorenz; VIRGINIA UNIV CHARLOTTESVILLE
|
| Purpose: To test the working hypothesis that SHP-1 is essential for controlling growth and differentiation of mammary epithelial cells and that its dysregulation contributes to the development of breast cancer. Scope: To biochemically and functionally characterize SHP-1 in human breast cancer cell lines and to define its biological function in normal epithelial cells. Major Findings: We have shown that SHP-1 associates with the EGFR in an EOF stimulation-dependent manner. In ... |
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| Protection of Normal Cells Against Toxic Effects of Chemotherapy by Reversible G1 Arrest |
JUL 2002 |
13 pages |
| Authors:
Khandan Keyomarsi; M D ANDERSON CANCER CENTER HOUSTON TX
|
| Treatment of cancer with chemotherapy and radiation therapy has severe side effects that damage healthy proliferating cells such as hematopoietic precursors, hair follicle, cells and the epithelial lining of the intestine. These side effects often limit the doses of chemotherapy administered, allowing tumor cells to gain growth advantage by escaping treatment and developing drug resistance. Since cancer therapy targets proliferating cells and tissues, all cells that proliferate, whether normal or ... |
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| The Use of Exercise to Increase CD4 (+) T Lymphocytes Following Chemotherapy Treatment for Breast Cancer |
JUN 2002 |
57 pages |
| Authors:
Andrea M. Mastro; PENNSYLVANIA STATE UNIV UNIVERSITY PARK
|
| Breast cancer is estimated to affect one in eight women in her lifetime. Treatment usually includes chemotherapy which can lead to several serious physical, emotional, and functional side effects. Along with the tumor cells, the normal, continually renewing cells of the lymphatic/immune hematopoietic system are targets for the cytotoxic drugs. One population of lymphocytes, CD4+T cells (T helper cells), major regulators of the immune system, are particularly susceptible to chemotherapy-induced ... |
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| An Automated Differential Blood Count System |
25 OCT 2001 |
|
| Authors:
Guclu Ongun; Ugur Halici; Kemal Leblebicioglu; Volkan Atalay; Meral Beksac; MIDDLE EAST TECHNICAL UNIV ANKARA (TURKEY) COMPUTER VISION AND NEURAL NETWORKS RESEARCH CENTER
|
 | While the early diagnosis of hematopoietic system disorders is view important in hematology, it is a highly complex and time consuming task. The early diagnosis requires a lot of patients to be followed-up by experts which, in general is in-feasible because of the required number of experts. The differential blood counter (DBC) system that we have developed is an attempt to automate the task performed manually by experts in routine. ... |
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| Retroviral Transfer of Human Mutated Thymidylate Synthase Gene into Hematopoietic Stem Cells for Protection from High-Dose Fluoropyrimidine Toxicity |
OCT 2001 |
17 pages |
| Authors:
Naoko Takebe; Robert Gallo; BALTIMORE UNIV MD
|
| In the field of breast cancer, the significant cytoreduction followed by consolidation chemotherapy has been accepted to reduce disease relapse in breast cancer. However, myelosuppression still impose restrictions on the optimization of this treatment modality. Insertion of drug resistance genes into hematopoietic progenitor cells offers an additional approach to allow further dose intensification and treatment post transplant. We have generated and characterized human thymidylate synthase (TS) mutants, one of which ... |
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| Determination of the Molecular Mechanisms Responsible for Abnormal CD43 Expression in Breast Cancer |
JUN 2001 |
11 pages |
| Authors:
Carl S. Shelley; MASSACHUSETTS GENERAL HOSPITAL BOSTON
|
| Human CD43 is a large, abundant and highly charged transmembrane molecule which inhibits intercellular interaction. Normally CD43 expression is restricted to the surface of hematopoietic cells. However, primary breast cancer tumors and their metastases also exhibit CD43 expression. Despite the linkage between breast cancer and abnormal CD43 expression, the molecular mechanisms responsible for this abnormal expression have never been investigated. The goal of this project is to elucidate these mechanisms. ... |
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| Effect of Reproductive History on Mammary Epithelial Biology |
MAY 2001 |
|
| Authors:
Lewis A. Chodosh; PENNSYLVANIA UNIV PHILADELPHIA
|
| Epidemiologic studies have repeatedly demonstrated that women who undergo a first full-term pregnancy early in life have a significantly reduced lifetime risk of breast cancer. Similar to humans, rodents also display parity-induced protection against breast cancer. We have used high-density DNA oligonucleotide microarrays to identify genes whose expression in the murine mammary gland is persistently altered as a consequence of an early first full-term pregnancy. The panel of differentially expressed ... |
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| Retroviral Transfer of Human Mutated Thymidylate Synthase Gene into Hematopoietic Stem Cells for Protection from High-Dose Fluoropyrimidine Toxicity |
OCT 2000 |
9 pages |
| Authors:
Naoko Takebe; Robert Gallo; MARYLAND UNIV BALTIMORE
|
| In the field of breast cancer, the significant cytoreduction followed by consolidation chemotherapy has been accepted to reduce disease relapse in breast cancer. However, myelosuppression still impose restrictions on the optimization of this treatment modality. Insertion of drug resistance genes into hematopoietic progenitor cells offers an additional approach to allow further dose intensification and treatment post transplant. We have generated and characterized human thymidylate synthase (TS) mutants, one of which ... |
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| The Role of SHP1 as a Tumor Suppressor Gene in Human Mammary Turmorigenesis |
AUG 1999 |
9 pages |
| Authors:
Chuan Gao; M D ANDERSON CANCER CENTER HOUSTON TX
|
| SHPl is a cytosolic protein tyrosine phosphatase containing two SH2 domains. It is highly expressed in hematopoietic cells and at lower levels in epithelium. We introduced SHPl, both in its wild type (wt) or a catalytic inactive (dn) form, into the human breast cancer cell line MDA-MB23 1, where SHP 1 expression is nearly lost. The overexpression of wt SHP1, but not dn SHPl, led to enhanced ... |
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| Diagnose und Therapie von Funktionsstoerungen menschlicher Leukozten nach Bestrahlung (Diagnosis and Therapy of Dysfunctions of Human Leukocytes After Irradiation) |
1999 |
191 pages |
| Authors:
W. Kaffenberger; D. Van Beuningen; BUNDESMINISTERIUM DER VERTEIDIGUNG BONN(GERMANY F R)
|
| Infections are an important aspect of the hematopoietic syndrome of acute radiation sickness. Pathophysiological infections arc caused by depletions and/or functional deficiencies of white blood cells as major carriers of cellular immunity. Polymorphonuclear neutrophilic granulocytes (PMN) are important as the first immunological barrier. Experiments were performed with isolated human PMN and with the promyelecytic HL-60 cell line, induced to differentiate along the granulocytic lineage with dimethyl sulfoxid. The respiratory burst ... |
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| Animal Studies of Residual Hematopoietic and Immune System Injury From Low Dose/Low Dose Rate Radiation and Heavy Metals |
SEP 1998 |
|
| Authors:
A. S. Yagunov; S. V. Tokalov; A. B. Chukhlovin; B. V. Afanassiev; ARMED FORCES RADIOBIOLOGY RESEARCH INSTBETHESDA MD
|
| The interaction of low dose/low dose rate radiation with heavy metals (in this report cadmium and lead) is important for at least two reasons: 1) radiation workers and populations exposed to unusually high background levels of radiation (e.g. Chernobyl and the Techa river in Russia) receive this type of exposure rather than the much better studied prompt high dose exposure, and 2) radiation accidents frequently involve the release of other ... |
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| A Pilot Study of Occupational Assessment of Air Force Personnel Exposure to Jet Fuel Before and After Conversion to JP-8 |
SEP 1998 |
53 pages |
| Authors:
Donna M. Olsen; David R. Mattie; William D. Gould; Frank Witzmann; Mark Ledbetter; AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH HUMAN EFFECTIVENESS DIRECTORATE
|
| The purpose of this study was to compare workers' health while JP-4 was still in use and after conversion to JP-8. Measures of effect were blood tests for liver, kidney and hematopoietic system function and serum protein analysis, neurocognitive function, and general health (history and physical exam). A cohort of 18 exposed and 18 non-exposed subjects, matched for gender and age, were measured 4 times over a period of 18 ... |
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| Expression and Activation of STAT Transcription Factors in Breast Cancer |
1998 |
227 pages |
| Authors:
John D. Schaber; UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD
|
| Endocrine therapy has proven a valuable approach to the treatment of breast cancer. In particular, antiestrogens have demonstrated significant improvement in survival rates, and have recently been shown to prevent breast cancer development in women in high-risk populations. Other endocrine or cytokine-based therapies, including glucocorticoids and interferons, which have been highly effective as adjuvant treatment of hematological cancers, have also shown promise in breast cancer. However, due to the less ... |
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| A Novel 3 Dimensional Biomaterial for the Production of Multiple Blood Cell Types. |
30 OCT 1997 |
26 pages |
| Authors:
Mark J. Pykett; CYTOMATRIX LLC CAMBRIDGE MA
|
| Support of wounded personnel is intimately tied to the availability of sufficient quantities of effective blood cells, including conventional red blood cells and platelets and novel therapeutic T lymphocytes and white blood cells. Phase I demonstrated the feasibility of utilizing a novel, biocompatible 3-D device, termed Cellfoam, to support the in vitro production of red blood cells, white blood cells and platelets from human hematopoietic progenitor cells (HPCs). Previous work ... |
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| Megakaryocytopoiesis in Stem Cell Transplantation |
OCT 1997 |
26 pages |
| Authors:
Isaac Cohen; NORTHWESTERN UNIV EVANSTON IL
|
| The overall objective of this research proposal is to investigate several laboratory protocols to improve megakaryocyte production for future clinical use of an autologous megakaryocyte-rich product. Conditioned medium from bone marrow stromal cultures enhances the thrombopoietin stimulated megakaryocyte production by at least 3-fold by a still unknown mechanism. We were able to rule out any role of stromal concentrations of thrombopoietin, interleukins 3 and 6, stem cell factor and heparan ... |
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| Expansion of Hematopoietic Stem Cells for Transplantation with flt3 Ligand |
AUG 1997 |
9 pages |
| Authors:
Sten E. Jacobsen; HIPPLE CANCER RESEARCH CENTER DAYTON OH
|
| In the last years promising results have emerged with regard to the ability of high dose chemotherapy to improve the prognosis of patients with advanced cancer of many types. Accordingly, a high number of clinical high dose chemotherapy trials are currently running on different cancers. Since the bone marrow toxicity resulting from such treatment is lethal, it is absolutely required to do a hematopoietic transplantation in these patients following the ... |
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| Preclinical Studies on the Potential Use of Swainsonine with High-Dose Chemotherapy in Humans: Chemoprotective and Hemarestorative Properties |
JUL 97 |
21 pages |
| Authors:
Sandra White; DUKE UNIV MEDICAL CENTER DURHAM NC
|
| The overall goal of these pre-clinical studies is to optimize the therapeutic potential of the novel agent, swainsonine, for utilization in the management of myelosuppression associated with high-dose chemotherapy, a method of treatment currently being used for human metastatic and high-risk primary breast cancer. Our initial studies have focused on determining the optimal dose, schedule and timing of swainsonine treatment to protect against the toxicity associated with multiple cycles of ... |
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| Estimation of Radiation Risk Based on the Concept of Individual Variability of Radiosensitivity |
JUN 96 |
202 pages |
| Authors:
E. E. Kovalev; O. A. Smirnova; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
|
| This report was prepared for the Defense Nuclear Agency under contract number DNA001-93-C-0152. A description and analysis of mathematical models developed for two critical systems, the hematopoietic and intestinal systems, are presented. The models, based on modem theories of regulation of the hematopoietic and intestinal epithelium systems, describe the dynamics of these systems in nonirradiated mammals and in mammals exposed to acute and chronic radiation. The first model uses the ... |
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| Combined Administration of Recombinant Human Megakaryocyte Growth and Development Factor and Granulocyte Colony-Stimulating Factor Enhances Multilineage Hematopoietic Reconstitution in Nonhuman Primates after Radiation-Induced Marrow Aplasia |
MAY 1996 |
8 pages |
| Authors:
Ann M. Farese; Pamela Hunt; Lisa B. Grab; Thomas J. MacVittie; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
|
| This study compared the therapeutic potential of recombinant, native versus pegylated megakaryocyte growth and development factor "rMGDF and PEG-rMGDF, respectively", as well as that of the combined administration of PEG-rMGDF and r-methionyl human granulocyte colonystimulating factor "r-metHuG-CSF" on hematopoietic reconstitution after 700 cGy 60Co gamma, total body irradiation in nonhuman primates. After total body irradiation animals received either rMGDF, PEG-rMGDF, r-metHuGCSF, PEG-rMGDF and r-metHuG-CSF, or HSA. Cytokines in all MGDF ... |
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| Megakaryocytopoiesis in Stem Cell Transplantation |
20 SEP 95 |
13 pages |
| Authors:
Isaac Cohen; NORTHWESTERN UNIV EVANSTON IL
|
| Following high-dose chemotherapy for the treatment of malignant disease, reinfusion of peripheral blood stem cells collected during treatment with growth factors usually results in delayed platelet engrafiment as compared to neutrophil recovery. The overall objective of this research proposal is to improve the capacity of stem cells to engrafi ;the megakaryocytic lineage. To achieve this goal we carried out ex vivo expansion of megakaryocyte (Mk) progenitors from human bone marrow ... |
|
| AFRRI Reports, First Quarter 1994 |
JUN 94 |
68 pages |
| Authors:
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
|
| Partial contents: Quinolone and glycopeptide therapy for infection in mouse following exposure to mixed-field neutron-gamma-photon radiation; Deficits in the sensitivity of striatal muscarinic receptors induced by (56)Fe heavy- particle irradiation: Further 'age-radiation' parallel; Synaptic transmission in the hippocampus: Critical role for glial cells; Single and combination cytokine therapies for treatment of radiation-induced hematopoietic injury: Effects of c- kit ligand and interleukin-3; Mast cell growth factor enhances multilineage hematopoietic recovery in ... |
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| Animal Models of Ionizing Radiation Damage |
01 JAN 92 |
|
| Authors:
Lynda L. Pippin; PATHOLOGY ASSOCIATES INC FREDERICK MD
|
| This report is a survey of the English language literature of radiation biology between 1947 and 1987, for the purpose of compiling a literature base on the effects of radiation on animals, which have yielded results that can expand our knowledge about similar radiation effects on human beings. Articles were sought that reported exposure of adult mammals to external sources of ionizing radiation, having endpoints that included effects on the ... |
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| Pathobiology of HIV in the Human Monocyte-Macrophage |
24 OCT 91 |
|
| Authors:
Jerome E. Groopman; NEW ENGLAND DEACONESS HOSPITAL BOSTON MA
|
| In the past year, we have made considerable progress in addressing specific aims of our proposal. We have studied the interaction of HIV with monocyte-macrophages with particular emphasis on comparing these interactions with those that occur in human T lymphocytes. We have found that there are cellular transcription factors which interact with the HIV LTR independent of NF-kappa B which could be important in virus expression in different cells. The ... |
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| Development of Hematopoietic Growth Factors for Use in Military Personnel |
12 JUL 91 |
3 pages |
| Authors:
Arthur J. Sytkowski; NEW ENGLAND DEACONESS HOSPITAL BOSTON MA
|
| We have continued our work on the new series of erythropoietin mutants. Interestingly, in sequencing the first five mutants that we derived, we discovered that another mutation had occurred spontaneously. In the short term, this has resulted in some delay in our studies, since we now feel it necessary to sequence not only the mutated domain of each cDNA, but rather the entire cDNA to verify the absence of other ... |
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| Development of Hematopoietic Growth Factors for Use in Military Personnel |
13 MAR 91 |
|
| Authors:
Arthur J. Sytkowski; NEW ENGLAND DEACONESS HOSPITAL BOSTON MA
|
| In order to focus on the putative active site of erythropoietin more carefully, we have begun to develop a series of single amino acid substitution mutants. Since our last report we have succeeded in deriving five new mutants, with six more to be produced. The mutants already in hand are currently being subjected to DNA sequencing to confirm their structure, and within the next two to three months the cDNAs ... |
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| Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes |
91 |
66 pages |
| Authors:
James J. Kenny; PROGRAM RESOURCES INC FREDERICK MD
|
| There are three main objectives of the research. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig- genes into human hematopoietic progenitors ... |
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| Development of Hematopoietic Growth Factors for Use in Military Personnel |
09 NOV 90 |
|
| Authors:
Arthur J. Sytkowski; HARVARD MEDICAL SCHOOL BOSTON MA
|
| This project is designed to develop second generation erythropoietin molecules with more favorable pharmacologic properties that can be applied to military personnel. Part of our strategy has been to study the structure of the molecule using a combination of chemical and molecular biologic techniques in order to rationally engineer new bio-molecules. When this grant began, we had evidence that amino acids 99-109 of the hormone were important for its biological ... |
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| Atlas of Tumor Pathology |
01 OCT 90 |
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| Authors:
Frences A. Pitlick; UNIVERSITIES ASSOCIATED FOR RESEARCH AND EDUCATION IN PATHOLOGY INC BETHESDA MD
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| This research grant is to provide the necessary editorial support to identify topics for future fascicles, invite recognized pathologists to serve as authors for these topics, to coordinate the receipt, review, and revision process for the authors' manuscripts, to copy edit the manuscripts for publication, and to prepare camera ready copy of the text for printing at the Armed Forces Institute of Pathology. This work is accomplished with the advice ... |
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| Development of Hematopoietic Growth Factors for Use in Military Personnel |
17 AUG 90 |
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| Authors:
Arthur J. Sytkowski; NEW ENGLAND DEACONESS HOSPITAL BOSTON MA
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| Mutation of amino acids 99-109 of the hormone drastically alters the hormone's biological activity. Our preliminary conclusion is that amino acids 99-109 are very important for biological activity. The goal of this project is to purify the B cell derived burst promoting activity (B-BPA) growth factor in order to obtain amino acid sequence, clone and express the gene, and initiate in vivo trials of this agent. We have begun to ... |
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