| Origins of DNA Replication and Amplification in the Breast Cancer Genome |
Sep 2012 |
43 pages |
| Authors:
Susan A Gerbi; Alexander Brodsky; Ben Raphael; BROWN UNIV PROVIDENCE RI
|
 | The major goal of this IDEA Expansion Breast Cancer Research grant is to test whether a correlation existsbetween sites of DNA amplification and estrogen receptor (ER) binding in the breast cancer genome. Correlations would support our hypothesis that ER adjacent to replication origins may interact with the replication machinery to drive DNA amplification, a hallmark of many cancers. Our Specific Aims are: (1) Map replication origins in the MCF-7 breast ... |
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| The Interplay Between Estrogen and Replication Origins in Breast Cancer DNA Amplification |
Sep 2012 |
23 pages |
| Authors:
Cinzia Casella; BROWN UNIV PROVIDENCE RI
|
| In order to study the role that estrogen might have in breast cancer DNA amplification, I started to build the cell lines that I will use as a model for the upcoming experiments. MCF7 Flp-In cell lines have been established through the selection of cells stably transfected to carry an FRT (Flippase Recombinase Target site) sequence, which subsequently will function as acceptor site for the integration of constructs with an ... |
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| Asymptotic Description of Damping Mistuning Effects on the Forced Response of Turbomachinery Bladed Disks |
31 Jul 2012 |
44 pages |
| Authors:
Carlos Martel; UNIVERSIDAD POLITECNICA DE MADRID (SPAIN)
|
| This is the final report of the research grant FA8655-11-1-3084 issued by the European Office of Aerospace Research and Development. This research effort is devoted to the analysis of the effect of damping mistuning on the forced response of turbomachinery bladed disks using asymptotic techniques. In particular it includes: (i) the derivation of the simplified Asymptotic Mistuning Model (AMM) for the general problem of the forced response of a bladed ... |
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| Horns as Particle Velocity Amplifiers |
Nov 2011 |
6 pages |
| Authors:
Dimitri M Donskoy; Benjamin A Cray; NAVAL UNDERSEA WARFARE CENTER DIV NEWPORT RI
|
| Preliminary measurements and numerical predictions reveal that simple, and relatively small, horns generate remarkable amplification of acoustic particle velocity. For example, below 2 kHz, a 2.5 cm conical horn has a uniform velocity amplification ratio (throat-to-mouth) factor of approximately 3, or, in terms of a decibel level, 9.5 dB. It is shown that the velocity amplification factor depends on the horn's mouth-to-throat ratio as well as, though to a lesser ... |
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| Long Island Sound Coastal Observatory: Assessment of Above-Water Radiometric Measurement Uncertainties Using Collocated Multi and Hyper-Spectral Systems |
14 Oct 2011 |
20 pages |
| Authors:
Tristan Harmel; Alexander Gilerson; Soe Hlaing; Alberto Tonizzo; Tom Legbandt; Alan Weidemann; Robert Arnone; Samir Ahmed; NAVAL RESEARCH LAB STENNIS DETACHMENT STENNIS SPACE CENTER MS OCEANOGRAPHY DIV
|
| The Long Island Sound Coastal Observational platform (LISCO) near Northport, New York, has been recently established to support validation of ocean color radiometry (OCR) satellite data. LISCO is equipped with collocated multispectral, SeaPRISM, and hyperspectral, HyperSAS, above-water systems for OCR measurements. This combination offers the potential for improving validation activities of current and future OCR satellite missions, as well as for satellite intercomparisons and spectral characterization of coastal waters. Results ... |
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| Identifying Breast Cancer Oncogenes |
Oct 2011 |
18 pages |
| Authors:
Yashaswi Shrestha; DANA-FARBER CANCER INST BOSTON MA
|
| Identification of novel drivers of cancer is necessary to understand the mechanisms of transformation as well as reveal more efficient targets for cancer therapy. Various subtypes of human cancer show activation of the RAS-MAPK pathway however, activating mutations in the pathway are frequent only in a few subtypes. For instance, although human breast tumors show activated RAS-MAPK signaling, activating mutation of RAS or members of the MAPK pathway is rare, ... |
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| Identification of Autoantibodies to Breast Cancer Antigens in Breast Cancer Patients |
Oct 2011 |
80 pages |
| Authors:
Leah Novinger; VERMONT UNIV BURLINGTON
|
| The purpose of this project is to identify breast cancer antigens and autologous antibodies that breast cancer patients make to those antigens. The scope of this project is the development of new targeted therapeutics to treat breast cancer. Screening over a million clones from a patient tumor cDNA expression library with autologous serum has identified over 440 possible positive proteins revealing autoantibodies to at least six SEREX antigens and at ... |
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| Multicast Parametric Synchronous Sampling |
Sep 2011 |
31 pages |
| Authors:
Stojan Radic; CALIFORNIA UNIV SAN DIEGO LA JOLLA DEPT OF ELECTRICAL AND COMPUTER ENGINEERING
|
| Parametric signal processing technology is described. Work on mixer synthesis, gain and conversion efficiency, signal multicasting and sampling is outlined in detail. The technology limits and its applications in multiplexing, demultiplexing, amplification, conversion and signal delay construction are described. Achievements include (1) longitudinal nonlinear waveguide characterization which resulted in unprecedented mixers surpassing 20 THz in bandwidth and greater than 20 dB gain, (2) ability to copy THz signals with impunity ... |
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| Effectiveness of Selected Native Plants as Competitors with Non-indigenous and Invasive Knapweed and Thistle Species |
Sep 2011 |
152 pages |
| Authors:
Thomas Smith; Ann L Hild; ENGINEER RESEARCH AND DEVELOPMENT CENTER CHAMPAIGN IL
|
| This work examined the response of native grass populations to long-term presence of non-indigenous invasive plant species (NIPS). Two native grass species common to arid and semi-arid rangelands were identified (alkalai sacaton [Sporobolus airoides] and needle and thread grass [Hesperostipa comata]) as remnants of native communities subjected to NIPS Russian knapweed [Rhaponticum repens]). Maternal grass individuals were collected to examine the influence of the invasions on population genetics and phenology ... |
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| Massively Parallel Rogue Cell Detection Using Serial Time-Encoded Amplified Microscopy of Inertially Ordered Cells in High-Throughput Flow |
Aug 2011 |
13 pages |
| Authors:
Dino Di Carlo; CALIFORNIA UNIV LOS ANGELES
|
| We aim to develop an instrument for high-throughput identification of rare circulating breast cancer cells to enable early detection and analysis of treatment effectiveness. To address this challenge, we have developed an automated flow-through single-cell optical microscopy system that can evaluate, diagnose, and screen a large population of cells in a short time. This method builds on an unique integration of (i) an ultrafast optical imaging modality known as serial ... |
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| Sensitive Detection Using Microfluidics and Nonlinear Amplification |
22 Jul 2011 |
12 pages |
| Authors:
Rustem F Ismagilov; CHICAGO UNIV IL
|
| Rapid detection of small particles such as pathogens, environmental pollutants, and microorganisms is important for both the security and health of society at large. The main goal of this proposal is to establish the scientific foundations for rapidly detecting small particles at low concentrations by combining controlled chemical autocatalytic amplification and stochastic confinement of small particles with the microfluidic expertise that has been developed in Pi's laboratories. We focus on ... |
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| How Alterations in the Cdt1 Expression Lead to Gene Amplification in Breast Cancer |
Jul 2011 |
29 pages |
| Authors:
Kenta Terai; VIRGINIA UNIV CHARLOTTESVILLE
|
| We examined the effects of Cdt1 for gene amplification by using inducible system. We Obtained and validated reagents for inducible Cdt1-expression-system. Furthermore, we identified appropriated dose of MTX for testing gene amplification Cdt2 is a key molecule for inhibiting Cdt1 activity. The depletion of Cdt2 induces DNA re-replication in several cell lines. During experiment, we Identified a reduction of PCNA monoubiquitination in Cdt2 depleted MCF7 cells.Exogenous Cdt2 rescued the effect ... |
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| Checkpoint Pathways Activated by Re-Replication in Breast Cancer Cells |
MAY 2011 |
49 pages |
| Authors:
Jie Lin; VIRGINIA UNIV CHARLOTTESVILLE
|
| Emi-1 has been known as an inhibitor of APC (anaphase promoting complex). The depletion of Emi-1 would prematurely activate APC, lead to simultaneous degradation of geminin and cyclin A, and induce re-replication at least in colon cancer cell lines and breast epithelial cell MCF10A. We proposed Emi-1 depletion can induce re-replication in breast cancer cells, lead to gene amplification and stimulate tumor growth. We showed earlier that the depletion of ... |
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| Enlisting Fuzzy-Wuzzy: Are Past Lessons Concerning Employing Irregulars Relevant Today? |
24 Mar 2011 |
44 pages |
| Authors:
Edward J Amato; AIR WAR COLL MAXWELL AFB AL
|
| America s dominance on the conventional battlefield compels its adversaries to engage our military in less-governed areas often dominated by tribal and traditional social networks. The U.S. military has historically employed indigenous irregular forces to defeat such enemies. These campaigns have been remarkably effective at countering adversaries, particularly where state security forces have been unwilling or unable to do so. Despite this, U.S. capabilities for employing indigenous irregular forces have ... |
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| Grover's Search Algorithm with an Entangled Database State |
Mar 2011 |
18 pages |
| Authors:
Paul M Alsing; Nathan McDonald; AIR FORCE RESEARCH LAB ROME NY INFORMATION DIRECTORATE
|
| Grover s oracle based unstructured search algorithm is often stated as given a phone number in a directory, find the associated name. This paper examines an amplitude amplification algorithm in which the user encodes the directory (e.g. names and telephone numbers) into an entangled database state, which at a later time can be queried on one supplied component entry (e.g. a given phone number t0) to find the other associated ... |
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| A Robust Method to Estimate Instantaneous Heart Rate from Noisy Electrocardiogram Waveforms |
Feb 2011 |
13 pages |
| Authors:
Andrei V Gribok; Xiaoxiao Chen; Jaques Reifman; ARMY MEDICAL RESEARCH AND MATERIEL COMMAND FORT DETRICK MD
|
| We propose a new algorithm for real-time estimation of instantaneous heart rate (HR) from noise-laden electrocardiogram (ECG) waveforms typical of unstructured ambulatory field environments. The estimation of HR from ECG waveforms is an indirect measurement problem that requires differencing, which invariably amplifies high-frequency noise. We circumvented noise amplification by considering the estimation of HR as the solution of a weighted regularized least squares problem, which, in addition directly provided analytically ... |
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| Hormonal Involvement in Breast Cancer Gene Amplification |
OCT 2010 |
40 pages |
| Authors:
Susan A. Gerbi; Alexander Brodsky; Ben Raphael; BROWN UNIV PROVIDENCE RI
|
| Our specific aims were to map origins of replication in the breast cancer genome and compare these sites with genomic positions that bind the estrogen receptor (ER) and genomic regions of DNA amplification. Correlations would support our hypothesis that ER adjacent to replication origins may interact with the replication machinery to drive DNA amplification, hallmark of many cancers. We began our experiments with the well-studied breast cancer cell line MCF7. ... |
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| Effectiveness of Three Decontamination Treatments Against Influenza Virus Applied to Filtering Facepiece Respirators |
Oct 2010 |
12 pages |
| Authors:
Michael B Lore; TeAnne L Brown; Steven H Hinrichs; Brian K Heimbuch; Joseph D Wander; NEBRASKA UNIV MEDICAL CENTER OMAHA
|
| Single-use filtering facepiece respirators (FFRs) are effective precautions against airborne pathogenic microorganisms; however, during pandemics the demand for FFRs may far exceed availability. Reuse of FFRs following decontamination has been suggested but few studies to date have addressed the feasibility. Concerns regarding biocidal efficacy, filter performance post decontamination and cost of decontamination may affect the adoption of reuse measures. This study examined the effectiveness of three energetic decontamination methods (ultraviolet ... |
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| Rapid Multi-Locus Sequence Typing Using Microfluidic Biochips |
12 May 2010 |
8 pages |
| Authors:
Timothy D Read; Rosemary S Turingan; Christopher Cook; Heidi Giese; Ulrich H Thomann; Catherine C Hogan; Eugen Tan; Richard F Selden; NAVAL MEDICAL RESEARCH CENTER ROCKVILLE MD BIOLOGICAL DEFENSE RESEARCH DIRECTORATE (BDRD ANNEX)
|
| Multiple locus sequence typing (MLST) has become a central genotyping strategy for analysis of bacterial populations. The scheme involves de novo sequencing of 6-8 housekeeping loci to assign unique sequence types. In this work we adapted MLST to a rapid microfluidics platform in order to enhance speed and reduce laboratory labor time. Methodology/Principal Findings: Using two integrated microfluidic devices, DNA was purified from 100 Bacillus cereus soil isolates, used as ... |
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| Age-Related DNA Methylation Changes and Neoplastic Transformation of the Human Prostate |
Jul-2009 |
22 pages |
| Authors:
Bernard Kwabi-Addo; HOWARD UNIV WASHINGTON DC
|
| There is abundant evidence to suggest that DNA methylation changes may appear earlier during prostate cancer development than genetic changes, as well as more commonly and consistently. The purpose of the present study is to investigate whether aberrant methylation in normal prostate tissues may in itself be a pathologic event that increases with age. Using methylated CpG island amplification coupled with CpG promoter microarray, I have identified several novel genes ... |
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| Benign Breast Disease: Toward Molecular Prediction of Breast Cancer Risk |
Jun 2009 |
150 pages |
| Authors:
Lynn C Hartmann; MAYO CLINIC ROCHESTER MN
|
| Optimal early detection and prevention strategies for breast cancer are predicated on our ability to identify individuals at significantly increased risk for this disease. The purpose of this Center was to bring molecular risk prediction for breast cancer into the clinical arena. The three areas of scientific inquiry included:(i) Establishment of a tissue repository of benign breast disease; (ii) Assessment of potential biomarkers of risk in this tissue set and ... |
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| Advanced Space-Based Detector Research at the Air Force Research Laboratory |
04-Mar-2009 |
12 pages |
| Authors:
D A Cardimona; P M Alsing; D H Huang; T Apostolova; W R GLass; C D Castillo; AIR FORCE RESEARCH LAB KIRTLAND AFB NM SPACE VEHICLES DIRECTORATE
|
| At the Space Vehicles Directorate of the Air Force Research Laboratory we are interested in the use of detectors in space for surveillance and situational awareness missions. Our primary interests are in observations of objects both on earth and in space, each of which has very different background requirements. In addition, the space environment itself is especially demanding of any sensor system that will be expected to work continuously for ... |
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| Satellite Reentry Control Via Surface Area Amplification |
Mar-2009 |
128 pages |
| Authors:
Salvador Aleman; AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING AND MANAGEMENT
|
| This project endeavors to find whether it is feasible to use an increase in surface area as a way of increasing the drag on an orbiting object, thus decreasing its orbital lifetime. The surface area increase can be achieved by an apparatus that deploys a balloon. The balloon will act as a parachute that will decrease the potential energy of the object through atmospheric drag. This is most effective by ... |
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| Identification and Characterization of Genomic Amplifications in Ovarian Serous Carcinoma |
Jan-2009 |
148 pages |
| Authors:
Tian-Li Wang; JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
|
| The purpose of this proposed study is to apply genome-wide technologies to analyze ovarian cancer genome and transcriptome. We have accomplished all the proposed tasks as stated in the original grant. They include generating digital karyotyping libraries from ovarian carcinomas and perform transcirptome analysis in each amplicon. This effort leads us to identify at least two novel candidate oncogenes, Rsf1 and Notch3, which were up-regulated in both genomic DNA and ... |
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| Comparison of the Noise Penalty of a Raman Amplifier Versus an Erbium-doped Fiber Amplifier for Long-haul Analog Fiber-optic Links |
17-Dec-2008 |
17 pages |
| Authors:
Preetpaul S Devgan; John F Diehl; Vincent J Urick; Keith J Williams; NAVAL RESEARCH LAB WASHINGTON DC
|
| A Raman amplifier is compared to an erbium-doped fiber amplifier for a 14 km analog fiber-optic link requiring the amplifier pump at the end of the link. The noise penalty for each amplifier at a given photocurrent is measured and compared. An analytical expression comparing the analog metrics as a function of noise penalty and photocurrent for different amplifiers is derived and used. The Raman amplifier has a lower noise ... |
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| Quantum Search and Beyond |
02-Jul-2008 |
41 pages |
| Authors:
Lov K Grover; LUCENT TECHNOLOGIES INC MURRAY HILL NJ
|
| Ten years ago, the quantum search algorithm was designed to provide a way of searching a space of N items in only N steps. In the last ten years, it has been used as a building block for numerous applications, both physical and algorithmic -- these are as diverse as precision measurement and communication complexity. It has been generalized to the amplitude amplification principle in which form it can be ... |
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| Dynamics of Marine Zooplankton: Social Behavior, Ecological Interactions, and Physically-Induced Variability |
FEB 2008 |
|
| Authors:
Ariane Verdy; MASSACHUSETTS INST OF TECH CAMBRIDGE
|
 | Marine ecosystems reflect the physical structure of their environment and of the biological processes they carry out. This leads to spatial heterogeneity and temporal variability, some of which is imposed externally, and some of which emerges from the biological processes themselves. The main focus of this thesis is on the formation of spatial patterns in the distribution of zooplankton arising from social interactions between individuals. In the Southern Ocean, krill ... |
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| Indirect Detection Of Bacillus Anthracis (Anthrax) Using Amplified Gamma Phage-Based Assays |
01 NOV 2007 |
152 pages |
| Authors:
Robert W. Reiman; COLORADO SCHOOL OF MINES GOLDEN
|
| The need for a simple, specific, sensitive, inexpensive, accurate, and rapid method to identify Bacillus anthracis became apparent during the Fall 2001 anthrax attacks which caused widespread panic and ultimately killed five individuals. The Centers for Disease Control and Prevention currently employs agar plate lysis by gamma phage and direct fluorescence assay to confirm the presence of Bacillus anthracis. These confirmatory methods require isolation of individual colonies from an overnight ... |
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| Anti-Androgen Receptor RNA Enzyme as a Novel Therapeutic Agent for Prostate Cancer in Vivo |
AUG 2007 |
18 pages |
| Authors:
Shuo Chen; TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
|
| Prostate cancer is the second leading cause of cancer death among men in the western world. Androgen plays a crucial role in the development and growth of normal prostate gland and prostate cancer. Action of androgen is mediated by an androgen receptor (AR) and the AR exerts androgen-regulated gene expression. Standard therapy relies on androgen ablation to remove or block the action of androgens. This therapy results in a regression ... |
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| Stimulated Brillouin Scattering (SBS) Suppression Techniques |
31 JUL 2007 |
28 pages |
| Authors:
Michael W. Zmuda; AIR FORCE RESEARCH LAB KIRTLAND AFB NM DIRECTED ENERGY DIRECTORATE
|
| In an effort to increase SBS threshold for future high power fiber amplifier applications, several mitigation techniques have been evaluated and modeled. Among these techniques include novel modulation schemes, core material modifications, and utilization of the core thermal differential during amplification. The most promising techniques were combined to provide an estimated increase in SBS threshold by over a decade. |
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| Limited Evaluation of an 802.11b Air-to-Air Wireless Data Link (Project Have HALO II) |
01 JUN 2007 |
53 pages |
| Authors:
Robert A. Volesky; Jason W. Geitgey; Jason C. Vap; Douglas O. Creviston; Morikazu Kikuchi; AIR FORCE FLIGHT TEST CENTER EDWARDS AFB CA
|
| This report presents the results of Project Have HALO II a limited evaluation of an 802.11b wireless air-to-air data link between two C-12C aircraft. This test program demonstrated the 802.11b wireless data link reception range under 1 Watt and 5 Watts of amplification, corresponding to 0.32 and 1.58 Watt effective isotropic radiated power. The test team also demonstrated the capability of transmitting still photos, streaming video, pre-recorded video and text ... |
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| Targeting of CD151 in Breast Cancer and in Breast Cancer Stem Cells |
01-Apr-2007 |
34 pages |
| Authors:
Martin E Hemler; DANA-FARBER CANCER INST BOSTON MA
|
| A mouse model for spontaneous breast cancer has been set up to analyze the role of CD151 during breast cancer progression. Using this model, which involves amplification of the ErbB2 oncogene, preliminary data was then obtained indicating that the absence of CD151 causes a substantial delay in the appearance of mouse mammary tumors. To confirm these preliminary results, we next set up a larger scale experiment to evaluate the role ... |
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| Comparison of Amplification Methods to Produce Affymetrix GeneChip(Trademark) Target Material |
APR 2007 |
9 pages |
| Authors:
Dennis Burian; Vicky White; Mark Huggins; Doris Kupfer; Dennis V. Canfield; James E. Whinnery; FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST
|
| Whole blood from living subjects is a convenient matrix to use as a source of RNA for microarray experiments with human subjects especially when subject material is collected at a location other than the collaborating site conducting the microarray work. Collection methods for whole blood that include stabilization of the RNA are known but suffer from issues of decreased sensitivity due to the large amount of globin RNA present from ... |
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| Downstream Impacts Due to the Extratropical Transition of Tropical Cyclones Over the Western North Pacific |
MAR 2007 |
119 pages |
| Authors:
Jonathan M. Dea; NAVAL POSTGRADUATE SCHOOL MONTEREY CA
|
| Analysis of the eddy kinetic energy budget for four cases of extratropical transition (ET) with North Pacific tropical cyclones (TCs) is conducted. The cases compare varying ET and midlatitude flow characteristics. Each case was examined to determine the impacts of eddy kinetic energy generation and/or transfer on downstream development in the midlatitude circulation. Typhoon Tokage (October 2004) was a large TC that moved into a high-amplitude midlatitude circulation. Energetics analysis ... |
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| Stochastic Resonance Investigation of Object Detection in Images |
DEC 2006 |
14 pages |
| Authors:
Daniel W. Repperger; Alan R. Pinkus; Julie A. Skipper; Christina D. Schrider; AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH HUMAN EFFECTIVENESS DIRECTORATE
|
| Object detection in images was conducted using a nonlinear means of improving signal to noise ratio termed "stochastic resonance" (SR). In a recent United States Patent application, it was shown that arbitrarily large signal to noise ratio gains could be realized when a signal detection problem is cast within the context of a SR filter. Signal-to-noise ratio measures were investigated. For a binary object recognition task (friendly versus hostile), the ... |
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| Novel Targets for the Diagnosis and Treatment of Breast Cancer Identified by Genomic Analysis |
DEC 2006 |
22 pages |
| Authors:
Carol A. Westbrook; BOSTON MEDICAL CENTER CORP MA
|
| Chromosomal rearrangements which result in localized increases of genetic material are frequent in breast cancer and occur consistently in certain genomic regions. The resulting increase in expression of genes contained within these amplifications contributes to the malignant phenotype. Such amplified genes such as Her2-Neu provide targets for diagnosis and for the development of inhibitory drugs. The purpose of this study is to use novel genomic technologies to find new genes ... |
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| Mutations in PUMA Gene Cause Prostate Cancer Development and Aggressiveness |
NOV 2006 |
11 pages |
| Authors:
Chien-An Hu; NEW MEXICO UNIV ALBUQUERQUE
|
| In the first year of this project, we surveyed the PUMA gene expression using RT-PCR amplification of RNA isolated from three PCa cell models, LNCaP, DU145 and PC3, BPH (benign prostate hyperplasia) cells or normal prostate tissues. Our results showed that expression of PUMA was relatively high in normal prostate tissues/cells but was significantly reduced in two PCa cell models, DU145 and PC3. In addition, to survey the PUMA gene ... |
|
| Amplification of Type II Cadherins in Prostate Cancer |
NOV 2006 |
25 pages |
| Authors:
Teresa L. Johnson-Pais; TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
|
| Genomic alterations of chromosome 18q have been observed in prostate cancer. This research focuses on analyzing the role of increased gene copy number at chromosome 18q22.1 in prostate cancer. We believe the key genes in this region are the type II cadherins. We are studying the role of overexpression of these genes, particularly cadherin 7 (CDH7), on the tumorigenicand invasive potential of prostate cancer cells. We have shown the minimal ... |
|
| Advanced Space-Based Detector Research at the Air Force Research Laboratory (PREPRINT) |
OCT 2006 |
7 pages |
| Authors:
P. M. Alsing; D. A. Cardimona; D. H. Huang; T. Apostolova; W. R. Glass; C. D. Castillo; AIR FORCE RESEARCH LAB KIRTLAND AFB NM SPACE VEHICLES DIRECTORATE
|
| At the Space Vehicles Directorate of the Air Force Research Laboratory we are interested in the use of detectors in space for surveillance and situational awareness missions. Our primary interests are in observations of objects both on earth and in space, each of which has very different background requirements. In addition, the space environment itself is especially demanding of any sensor system that will be expected to work continuously for ... |
|
| Breast Cancer in Three Dimensions: Revealing Telomere Dysfunction in Breast Cancer |
SEP 2006 |
144 pages |
| Authors:
William Foulkes; MCGILL UNIV HEALTH CENTER MONTREAL (QUEBEC)
|
| The structural re-modeling of the mammalian nucleus is a key feature of cancer cells. Such reorganization of the nucleus impacts the genomic integrity of the cell. Our focus is on the genetic alterations that affect the telomeres (the ends of chromosomes) in breast cancer. In this study, we wished to determine if mutations in either of the two main breast cancer susceptibility genes, known as BRCA1 and BRCA2 can influence ... |
|
| Strong Optical Injection Locking of Edge-Emitting Lasers and Its Applications |
18 AUG 2006 |
158 pages |
| Authors:
Hyuk-Kee Sung; CALIFORNIA UNIV BERKELEY DEPT OF ELECTRICAL ENGINEERING AND COMPUTER SCIENCE
|
| Semiconductor lasers are essential components that enable high-speed long-haul communication and have been widely used for various applications in photonics technology. Semiconductor lasers under optical injection locking exhibit superior performance over free-running lasers and provide useful applications not achievable through the free-running lasers. The performance of injection-locked lasers has been found to be significantly improved with stronger injection. In this dissertation, the characteristics and applications of semiconductor lasers under strong ... |
|
| The Antemortem Detection and Conformational Switches of Prion Proteins |
JUL 2006 |
40 pages |
| Authors:
David Schubert; SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA
|
| Blood from animals with prion disease contain low levels of prion infectivity, which primarily resides in the white blood cells (WBCs). We have developed a method that combines isolation of WBCs and cell blotting of PrPsc to detect individual cells that contain PrPsc. Sensitivity studies suggest that it can detect as low as 10 prion-infected cells in 5 x 105 WBCs. The assay is able to detect the prion-infected cells ... |
|
| Expression and Genomic Profiling of Minute Breast Cancer Samples |
JUL 2006 |
70 pages |
| Authors:
G. M. Makrigiorgos; DANA-FARBER CANCER INST BOSTON MA
|
| To be able to apply the newest genetic analysis technologies in breast cancer research, enough DNA material must be available to perform this analysis. Often this is impossible with the minute amounts of tissue obtained via fine needle aspiration or laser capture microdissection currently the best methods available for removal of small amounts of tissue. Therefore whole genome-based DNA amplification techniques (e.g. PCR) are essential. The aim of this proposal ... |
|
| Halsey at Leyte Gulf: Command Decision and Disunity of Effort |
16 JUN 2006 |
131 pages |
| Authors:
Kent S. Coleman; ARMY COMMAND AND GENERAL STAFF COLL FORT LEAVENWORTH KS
|
| In October 1944, US forces executed amphibious landings on the Japanese-occupied island of Leyte in the central Philippines. Japanese naval forces, severely outnumbered by the US Third and Seventh Fleets, attempted to stop the invasion by attacking US amphibious shipping in Leyte Gulf. Due to the divided US area commands in the Pacific theater during World War II, the Third and Seventh Fleet commanders, Adm. Halsey and Vice Adm. Kinkaid, ... |
|
| A Digital Radio Receiver for Ionospheric Research |
01 JUN 2006 |
|
| Authors:
H. G. James; COMMUNICATIONS RESEARCH CENTRE OTTAWA (ONTARIO)
|
| Compared with the experience of the past few decades, ionospheric research in the near future will depend less on new science-dedicated orbital platforms and more on networks of ground facilities. An important role of scientific spacecraft in this context will be to provide space perspectives, as part of space-ground coordinated studies. The enhanced Polar Outflow Probe (ePOP) instrument suite will be launched on the Canadian CASSIOPE spacecraft in early 2008 ... |
|
| Response Amplification of Idealized Powerhouse Substructures to Earthquake Ground Motions |
JUN 2006 |
202 pages |
| Authors:
Robert M. Ebeling; Elvin J. Perez-Marcial; Donald E. Yule; ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS INFORMATION TECHNOLOGY LAB
|
| This report summarizes an investigation of the seismic response of Corps powerhouse structures. Research results include (1) the characterization and organization of the range in geometry of Corps powerhouse substructures for generator bays and service/erection bays; (2) development of representative, idealized substructure geometries for these types of structures; (3) construction of a series of finite element models of the substructures and subjecting these substructures to earthquake acceleration time-histories representing central/eastern ... |
|
| Effect of Side Jets in a Supersonic Flow Measured and Calculated on a Flat Plate and a Generic Missile Configuration |
May-2006 |
34 pages |
| Authors:
Anke Kovar; Erich Schulein; GERMAN AEROSPACE CENTER GOTTINGEN (GERMANY)
|
| An investigation on side jets in an M = 5.0 flow was conducted on a flat plate as well as on a generic missile configuration. Surface pressure measurements, oil flow visualisations, force measurements and associated numerical simulations gave insight into the interaction of different jet configurations and their influence on the efficiency of side jet control described by the amplification factor. The analysis of the data especially in the wake ... |
|
| Combined Singularity Avoidance for Variable Speed Control Moment Gyroscope Clusters |
18 APR 2006 |
16 pages |
| Authors:
David J. Richie; Vaios J. Lappas; Sajjad Asghar; Phil L. Palmer; SURREY UNIV GUILDFORD (UNITED KINGDOM)
|
| Variable Speed Control Moment Gyroscopes (VSCMGs), defined by Schaub in [1], effectively applied to combined energy storage and attitude control in [2,3], and further studied by Yoon and Tsiotras in [4,5], can avoid attitude singularities by varying wheel speed. However, this device's key advantage lies in torque amplification via gimballing (i.e. operating in its CMG mode). The primary limitation of this CMG mode, though, is the existence of gimbal-lock singularity ... |
|
| VP35 Knockdown Inhibits Ebola Virus Amplification and Protects Against Lethal Infection in Mice |
MAR 2006 |
12 pages |
| Authors:
Sven Enterlein; Kelly L. Warfield; Dana L. Swenson; David A. Stein; Jeffrey L. Smith; C. S. Gamble; Andrew D. Kroeker; Patrick L. Iversen; Sina Bavari; Elke Muehlberger; PHILIPPS UNIV MARBURG (GERMANY)
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| Phosphorodiamidate morpholino oligomers (PMO) are a class of uncharged single-stranded DNA analogs modified such that each subunit includes a phosphorodiamidate linkage and morpholine ring. PMO antisense agents have been reported to effectively interfere with the replication of several positive-strand RNA viruses in cell culture. The filoviruses, Marburg virus and Ebola virus (EBOV), are negative-strand RNA viruses that cause up to 90% lethality in human outbreaks. There is currently no commercially ... |
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| Identification and Characterization of Genomic Amplifications in Ovarian Serous Carcinoma |
Jan-2006 |
22 pages |
| Authors:
Tian-Li Wang; JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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| This proposal is to apply genome-wide technologies to analyze ovarian cancer genome and transcriptome in parallel in the same ovarian tumors to reveal genes with concurrent genomic amplification and transcription upregulation. We propose to use digital karyotyping as the discovery tool to identify genomic amplification in ovarian carcinomas, and based on the results from 7 digital karyotyping libraries, we are able to identify several novel amplifications with high frequency as ... |
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