| P270 AND THE SWI/SNF Complex in Breast Cancer |
JUN 2002 |
8 pages |
| Authors:
Xiaomei Wang; Elizabeth Moran; TEMPLE UNIV PHILADELPHIA PA
|
 | Breast cancer arises from a series of harmful mutations to genes important for the normal regulation of cell growth and differentiation. Identification of the gene products whose loss is important in the development of the cancer is the primary means of determining who is at risk. There is an acute need for a more comprehensive understanding of the gene products that contribute to regulation, and the consequences of their failures. ... |
|
| Exploiting IR-Inducible NQ01 Levels Using B-Lapachone, A Novel Apoptotic Agent |
APR 2002 |
69 pages |
| Authors:
David A. Boothman; CASE WESTERN RESERVE UNIV CLEVELAND OH
|
| We theorize that Beta-lapachone is an effective agent against prostate cancer (CaP) cells/tumors. It kills cells by targeting elevated NQ01 levels in CaP tissue. Beta-Lapachone targets ionizing radiation (IR)-inducible NQ01. Normal cells with little or no NQ01 levels are spared, while IR-treated CaP cells expressing NQ01 are killed by apoptosis. Beta-Lapachone killing of CaP tumors is independent of p53, pRb, cell cycle status, or hormone (non) responsiveness and involves calpain ... |
|
| Thermal Properties of Mineralized and Non Mineralized Type I Collagen in Bone |
APR 2002 |
7 pages |
| Authors:
L. F. Lozano; M. A. Pena-Rico; H. Jang-Cho; A. Heredia; E. Villarreal; CIUDAD UNIVERSITARIA MEXICO DF (MEXICO)
|
| The research about the structural stability of bone, as a composite material, compromises a complete understanding of the interaction between the mineral and organic phases. The thermal stability of human bone and type I collagen extracted from human bone by different methods was studied in order to understand the interactions between the mineral and organic phases when is affected by a degradation/combustion process. The experimental techniques employed were calorimetry and ... |
|
| The Single Prostate Cell Transcriptome as Biological Assay |
MAR 2002 |
38 pages |
| Authors:
Peter S. Nelson; FRED HUTCHINSON CANCER RESEARCH CENTERSEATTLE WA
|
| Technological advances in robotics and image analysis software have expanded the capabilities of large-scale strategies to provide for a more complete analysis of molecular differences between tissues or cells of interest. The research accomplished to date has demonstrated the ability to reproducibly isolate defined prostate cell populations by microdissection and flow cytometry. Gene expression studies of the cells purified by flow-cytometry reveal an altered expression profile that we believe results ... |
|
| Simulation of Protein and Peptide-Based Biomaterials |
13 FEB 2002 |
5 pages |
| Authors:
Valerie Daggett; UNIV OF WASHINGTON SEATTLE DEPT OF MEDICINAL CHEMISTRY
|
| The overall goal of the proposed research is to pursue realistic molecular modeling studies of the stability, dynamics, structure, function, and folding of proteins and protein-based biomaterials in solution. Simulations of elastin-based polymers will investigate the driving forces and mechanism for the unusual temperature-dependent conformational changes, knowledge that will assist in redesign of this system for specific applications and the design of other such molecules. Studies of the stabilities of ... |
|
| Molecular Mechanisms of Dietary Fatty Acids on Breast Cancer Growth and Development |
FEB 2002 |
59 pages |
| Authors:
Michael W. Kilgore; KENTUCKY UNIV LEXINGTON
|
| The link between diet and breast cancer has been long postulated and recently, extensively investigated. Identifying a molecular mechanism linking diet and breast cancer, however, has remained elusive. Recently, we identified an isoform of the cellular receptor called the peroxisomal proliferator- activated receptor gamma (PPAR gamma) in human breast cancer cell lines. Activation of PPAR can alternatively lead to tumor induction or differentiation into a more benign state, depending on ... |
|
| Blockade of Tumor Cell TGF-Betas: A Strategy to Reverse Antiestrogen Resistance in Human Breast Cancer |
JAN 2002 |
112 pages |
| Authors:
Carlos L. Arteaga; VANDERBILT UNIV MEDICAL CENTER NASHVILLE TN
|
| Transforming growth factor beta (TGF beta) is a pleiotropic growth factor, which plays a critical role in modulating cell growth, differentiation and plasticity. There is increasing evidence that after transformed cells lose their sensitivity to TGF beta-mediated growth inhibition, autocrine TGF Beta signaling may potentially promote tumor cell motility, invasiveness, and metastatic progression. In order to understand the molecular mechanisms by which autocrine TGF beta may selectively contribute to tumor ... |
|
| NF2 in Hrs-Mediated Signal Transduction |
NOV 2001 |
23 pages |
| Authors:
Stefan M. Pulst; CEDARS-SINAI MEDICAL CENTER LOS ANGELESCA
|
| We have identified Hrs (hepatocyte growth factor-regulated tyrosine kinase substrate) as an NF2 binding protein using the yeast two-hybrid system. Hrs is also known to interact with STAM (signal transduction adaptor molecule. Hrs appears to have growth suppressing functions at least in part mediated via binding to STAM with a resulting reduction in DNA synthesis. Progress is discussed in order of the three specific aims that were proposed originally: (1) ... |
|
| Proto-oncogene PML and Tumor Evasion in Prostate Cancer |
NOV 2001 |
122 pages |
| Authors:
Pan Zheng; OHIO STATE UNIV COLUMBUS
|
| This is the second annual report on the grant "Proto-oncogene PML and tumor evasion in prostate cancer". The purpose of the grant proposal is to identify the molecular mechanisms of tumor evasion of host anti-tumor immunity. We proposed to identify the antigen presentation defects in human prostate cancer samples and to use mouse prostate cancer model (TRAMP mice) to study the immune regulation and immune tolerance in prostate cancer. In ... |
|
| Transcriptional Regulation and Targeting of NF1 Gene Expression |
OCT 2001 |
17 pages |
| Authors:
David I. Rodenhiser; LONDON REGIONAL CANCER CENTRE (ONTARIO)
|
| An essential requirement in understanding how genes contribute to genetic disease is the thorough knowledge of the mechanisms controlling gene expression at the level of transcription. In this project we are identifying and functionally characterizing the NF1 regulatory region, and evaluating the molecular mechanisms that regulate NF1 transcription during normal cellular differentiation and the events leading to NF1 -related neoplasia. Overlapping regions of the NFl proximal promoter have been cloned ... |
|
| Molecular Diffuse Optical Tomography for Early Breast Cancer Detection and Characterization |
OCT 2001 |
51 pages |
| Authors:
Vasilis Ntziachristos; MASSACHUSETTS GENERAL HOSPITAL BOSTON
|
| Herein we demonstrate the construction of the first reported fluorescence animal tomographer for molecular investigations of cancer-associated expression patterns. Using inversion techniques that account for the diffuse nature of photon propagation in tissue and near infrared fluorescent molecular beacons we were able to obtain three-dimensional in-vivo images of cathepsin B expression of orthopic gliomas. We demonstrate that fluorescent probes, activated by carcinogenesis, can be detected with high positional accuracy and ... |
|
| Exploiting an NQ01-Directed, Calpain-Medicated Apoptotic Pathway for Breast Cancer Therapy |
OCT 2001 |
70 pages |
| Authors:
Collen Tagliarino; David A. Boothman; CASE WESTERN RESERVE UNIV CLEVELAND OH
|
| The purpose of this proposal is to further understand the molecular mechanisms of (beta-lap-induced apoptosis. We examined alterations in intracellular Ca2+ homeostasis using NQOl-expressing MCF-7 cells. Beta-Lap- exposed MCF-7 cells exhibited an early increase in intracellular Ca2+, from endoplasmic reticulum Ca2+ stores. BAPTA-AM, an intracellular Ca2+ chelator, blocked early increases in Ca2+ levels and inhibited (3-lap-mediated mitochondrial membrane depolarization, intracellular ATP depletion, specific and unique substrate proteolysis, and apoptosis. The ... |
|
| Molecular Determinants of Cellular Sensitivity to Flavopiridol, an Anti- Cell Signaling Anticancer Agent |
OCT 2001 |
16 pages |
| Authors:
Colin R. Campbell; MINNESOTA UNIV MINNEAPOLIS
|
| Flavopiridol (FP) is an investigational new drug currently in Phase II clinical trials for the treatment of solid tumors (Senderowicz et al., 1998). The precise mechanism of action of this compound is unknown, however, it is known to inhibit several members of the cyclin-dependent protein kinase family, and to induce cell death (Senderowicz, 1999, Schrump, et al., 1998, Bible and Kaufmann, 1996). The objective of this proposal is to gain ... |
|
| Overexpression of IL-4 Signaling Pathway to Inhibit Breast Tumor Growth |
OCT 2001 |
34 pages |
| Authors:
Jill M. Gilroy; TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO
|
| The purpose of this research project was originally to characterize growth inhibitory effects of interleukin-4 (IL-4) in breast cancer cells and identify key signaling molecules that may be targets for future strategies to enhance the negative growth effects of IL-4. The original investigators have reported that IL-4-mediated growth inhibition is associated with increased apoptosis and that IL-4 activates two important signaling molecules, IRS-i and STAT6. Inhibition of IRS-i does not ... |
|
| Molecular Mechanisms of Schwann Cell Proliferation in NF1 |
SEP 2001 |
136 pages |
| Authors:
George H. DeVries; CHICAGO ASSOCIATION FOR RESEARCH AND EDUCATION IN SCIENCE HINES IL
|
| Neurofibromatosis type 1 (NF 1) is a genetic disorder characterized by Schwann cell tumors called neurofibromas, which can potentially become malignant to form neurofibrosarcomas. Previously, our laboratory has reported that Schwann cells derived from neurofibromas abnormally express high levels of the receptor tyrosine kinase c-Kit, which contributes weakly to Schwann cell proliferation (Badache et at, 1998a). It is possible that c-Kit plays an important role during development and that its ... |
|
| Mechanisms of Mechano-Transduction Within Osteoblasts |
SEP 2001 |
89 pages |
| Authors:
Louis C. Gerstenfeld; BOSTON UNIV MA
|
| Mechanical stimulation is crucial to the homeostasis of adult bone density and mass. The hypothesis of this proposal is that bone cells sense their mechanical environment through specific cell surface receptors (integrins) that interact with specific extracellular matrix (ECM) proteins (osteopontin, bone sialoprotein, and fibronectin) that are the ligands for these receptors. We propose that the expression of these proteins is regulated in response to both cellular interactions with the ... |
|
| Modulation of Androgen-Induced Oxidative Stress Responses as a Chemopreventive Measure in Prostate Cancer |
SEP 2001 |
24 pages |
| Authors:
Todd A. Thompson; WISCONSIN UNIV-MADISON
|
| We have previously reported that androgens induce oxidative stress in the androgen-responsive LNCaP prostate carcinoma cell line. We hypothesize that androgen-induced changes in prostate redox status may contribute to prostate carcinogenesis. Chemopreventive measures may reduce the development of prostate cancer. This study addresses several molecular mechanisms implicated in cellular redox status. Intracellular calcium levels were increased by androgen exposure, which could be inhibited by vitamin E treatment. Activity of the ... |
|
| Regulation and Function of Pak Signaling in Ovarian Cancer |
AUG 2001 |
7 pages |
| Authors:
Ulla G. Knaus; SCRIPPS RESEARCH INST LA JOLLA CA
|
| The identification of genes, which play a critical role in malignant transformation of the ovarian epithelium, will allow developing molecular tools for detection or intervention of altered activities in ovarian cancer, a cancer, which is often discovered in a late stage and with poor prognosis. Protein kinases such as p2l- activated kinases (Paks) are valuable targets for pharmacological intervention. Our project will elucidate alternative molecular mechanisms, present in ovarian cancer ... |
|
| Role of Hunk and Punc in Breast Cancer and Mammary Development |
AUG 2001 |
88 pages |
| Authors:
Lewis A. Chodosh; PENNSYLVANIA UNIV PHILADELPHIA
|
| Major insights into the molecular mechanisms of cancer have been obtained by studies of a family of regulatory molecules known as protein kinases. Many protein kinases serve as relays for signals in the cell that regulate normal growth and cellular function. In addition, several members of this family of molecules have previously been shown to be involved in the development of breast cancer in humans. We have cloned two novel ... |
|
| Evaluation of the Use of DNA Adduct Dosimetry to Optimize the Timing of High Dose Therapy for Breast Cancer |
AUG 2001 |
59 pages |
| Authors:
Richard B. Everson; WAYNE STATE UNIV DETROIT MI
|
| For drugs that interact with DNA, measures of DNA damage can assess the intracellular availability of active drug at a critical molecular target. Measurements of DNA damage should reflect the integrated effect of all resistance factors, including both recognized mechanisms and uncharacterized mechanisms. Thus, molecular measures of DNA damage could provide an important tool for elucidating the time course of complex changes in resistance factors. Motivated by a recent clinical ... |
|
| Comparative Study of Molecular Mechanisms of Skin Irritation After Acute Exposure to m-XYLENE in Rats and Guinea Pigs |
AUG 2001 |
36 pages |
| Authors:
P. G. Gunasekar; J. N. McDougal; J. V. Rogers; C. M. Garrett; M. B. Kabbur; W. W. Brinkely; WRIGHT STATE UNIV DAYTON OH DEPT OF PHARMACOLOGY AND TOXICOLOGYY
|
| Organic solvents like xylene are recognized as skin irritants after dermal exposure. The molecular responses to organic solvents that result in acute irritation are not understood. In the present study, we compared and quantified the molecular responses of rat and guinea pigs skin to xylene irritation, since these species differ in their response to other chemicals. We also determined which animal model was more appropriate for predicting xylene-induced skin irritation. ... |
|
| Negative Regulation of Tumor Suppressor p53 Transcription in Breast Cancer Cells |
JUL 2001 |
37 pages |
| Authors:
Jingwen Liu; PALO ALTO INSTITUTE FOR RESEARCH AND EDUCATION INC CA
|
| Previously we showed that the transcription of the p53 gene in breast cancer cells was down regulated by OM. To elucidate the molecular mechanisms underlying the OM effect on p53 transcription, in this report, we dissected the p53 promoter region and analyzed the p53 promoter activity in breast tumor cells. We showed that deletion of the 5'-flanking region of the p53 promoter from -426 to -97 did not affect the ... |
|
| A Mouse Model for Prostate Cancer |
JUL 2001 |
26 pages |
| Authors:
Cory Abate-Shen; ROBERT WOOD JOHNSON MEDICAL SCHOOL PISCATAWAY NJ
|
| Mouse models of carcinogenesis have provided significant insights into the molecular mechanisms of tumor suppressor gene function. Our goal is to develop mouse models that recapitulate early stages of prostate carcinoma. Using such models we have demonstrated that the Nkx3.1 homeobox gene represents a prostate-specific tumor suppressor that undergoes epigenetic inactivation through loss of protein expression. Loss-of-function of Nkx3.1 in mice results in histopathological defects that resembles prostate cancer initiation ... |
|
| Role of Murine BRCA1 Protein Interactions in DNA Repair |
JUL 2001 |
12 pages |
| Authors:
Robert B. Boxer; Lewis A. Chodosh; PENNSYLVANIA UNIV PHILADELPHIA
|
| Amplification of the c-MYC oncogene has been found in five to twenty percent of breast tumors However, the molecular mechanisms by which c-MYC contributes to tumorigenesis is not well understood. To address this question, we have used the tetracycline regulatory system to conditionally express the c- MYC oncogene in the mammary epithelium of bitransgenic mice. To determine whether c-MYC induced mammary tumors remain dependent on c-MYC for maintainenance of the ... |
|
| Nuclear Patch Clamping for Determining Ion Channel Activities of Bcl Apoptosis Proteins in Endoplasmic Reticulum and Nuclear Envelope Intracellular Membranes |
JUL 2001 |
9 pages |
| Authors:
J. K. Foskett; PENNSYLVANIA UNIV PHILADELPHIA
|
| Apoptosis, the morphological and biochemical manifestation of programmed cell death, plays a critical role in maintaining homeostasis of tissue and organ cell number, and is involved in differentiation, growth and development (1-4). Mammary gland physiology is strongly influenced by apoptosis in both normal and pathologic states. Involution of the lactating gland is due to apoptosis of differentiated epithelial cells, and an emerging hypothesis is that dysfunction of the apoptotic pathways ... |
|
| Promoter Regions Determining Over-expression of Metalloproteinase Genes in Breast Cancer |
JUL 2001 |
38 pages |
| Authors:
James Lyons; ROYAL PRINCE ALFRED HOSPITAL CAMPERDOWN (AUSTRALIA)
|
| The aim of this project is to determine which parts of the matrix metalloproteinase (MMP) genes cause those genes to be over-expressed in breast cancer, contributing to invasion and metastasis. It was determined that breast cancer cells can be classified into two types: one type retains its epithelial characteristics, the other has lost them by undergoing an epithelial-mesenchymal transition (EMT). The MMPs in each cell type are up-regulated by distinct ... |
|
| Role of Nip3 in Apoptosis and Chemoresponses of Breast Cancer Cells |
JUL 2001 |
18 pages |
| Authors:
Bin Guo; BURNHAM INST LA JOLLA CA
|
| The overall objective of this project is to investigate the molecular mechanisms of Nip3's function in apoptosis and the role of Nip3 in chemoresponses of breast cancer cells. The first aim is to determine whether Nip3 acts directly or indirectly to induce cell death. The results showed that Nip3 does not directly induce cell death in yeast. Next, the role of Bax in Nip3 action was evaluated in the induction ... |
|
| Regulation of C-myc Gene Expression by Potassium Channel Blocker Quinidine in MCF-7 Human Breast Cancer Cell Line |
JUL 2001 |
25 pages |
| Authors:
Zaroui Melkoumian; WEST VIRGINIA UNIV RESEARCH CORP MORGANTOWN*
|
| C-myc is a protooncogene whose gene product has a regulatory role in cell cycle progression, cellular differentiation, and apoptosis. The abnormal expression of c-myc was reported in 32% of breast cancers, suggesting its importance in the genesis and/or progression of breast cancer. Therefore, it is important to understand the precise molecular mechanisms of c-myc regulation. The goal of this proposal is to elucidate how potassium channel blocking agent, quinidine, regulates ... |
|
| Parity-Induced Protection Against Breast Cancer |
JUL 2001 |
109 pages |
| Authors:
Celina M. D'Cruz; PENNSYLVANIA UNIV PHILADELPHIA
|
| A woman's lifetime risk of developing breast cancer is significantly reduced by an early first full-term pregnancy. Thus, an early first childbirth is one of the most effective naturally occurring protective events that can diminish a woman's risk of breast cancer and is a candidate for targeted chemopreventive strategies. Although there is extensive epidemiological evidence in support of parity-induced protection against breast cancer, very little is known about the molecules ... |
|
| Molecular Disruption of Breast Tumor Angiogenesis |
JUL 2001 |
22 pages |
| Authors:
Paul J. Higgins; ALBANY MEDICAL COLL NY
|
| Findings in mice genetically-engineered to be deficient in the expression of genes that comprise the plasmin activation cascade confirmed the importance of plasminogen activator inhibitor type-1 (PAI-1) in tumor-induced angiogenesis. We found that endothelial cells must express PAI-1 to undergo angiogenesis upon co-culture with human breast tumor cells. PAI-1, thus, is an important candidate target for gene therapy of human cancers. Year 01 work determined the effects of expression vector-driven ... |
|
| Molecular Mechanisms of Calcium Hydroxyapatite Crystal Induced Nitrogenesis in Breast Cancer |
JUL 2001 |
36 pages |
| Authors:
Maria Morgan; Geraldine McCarthy; ROYAL COLL OF SURGEONS IN IRELAND DUBLIN
|
| For some time microcalcifications associated with breast lesions were considered to represent an epiphomenon, useful only as a radiological indicator of breast carcinoma. However, the data presented here shows that HA crystals are capable of exerting significant biological effects on surrounding cells. In this study we report that HA increases mitogenesis in both normal and malignant mammary cell lines. Particles of latex beads, of similar size and concentration to HA ... |
|
| Molecular Mechanism for Loss of Cell Adhesion in HER-2/neu Overexpressing Tumor Cells |
JUL 2001 |
13 pages |
| Authors:
Michael F. Press; UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES
|
| The HER-2/neu proto-oncogene is amplified and overexpressed in approximately 25% of breast cancers. Amplification and overexpression of HER-2/ neu is correlated with poor patient prognosis, lack of responsiveness to tamoxifen treatment, responsiveness to adriamycin chemotherapy and responsiveness to Herceptin anti-HER-2/neu immunotherapy. In this proposal we are characterizing changes in cell adhesion related to HER-2/neu overexpression. We have engineered two human cell lines to overexpress HER-2/neu Relative to parental control cell ... |
|
| Controlled Forward and Inverse Chemical Dynamics |
06 JUN 2001 |
17 pages |
| Authors:
Herschel Rabitz; PRINCETON UNIV NJ DEPT OF CHEMISTRY
|
| This research explored a variety of interrelated topics in the general area of controlling molecular dynamics phenomena with tailored laser pulses. The research concerned the development of new algorithms for the design of such laser pulses, as well as for directly aiding the laboratory implementation of laser-controlled molecular processes. In addition, studies were carried out to explore the possibility of using data from control experiments to provide detailed insights into ... |
|
| Determination of the Molecular Mechanisms Responsible for Abnormal CD43 Expression in Breast Cancer |
JUN 2001 |
11 pages |
| Authors:
Carl S. Shelley; MASSACHUSETTS GENERAL HOSPITAL BOSTON
|
| Human CD43 is a large, abundant and highly charged transmembrane molecule which inhibits intercellular interaction. Normally CD43 expression is restricted to the surface of hematopoietic cells. However, primary breast cancer tumors and their metastases also exhibit CD43 expression. Despite the linkage between breast cancer and abnormal CD43 expression, the molecular mechanisms responsible for this abnormal expression have never been investigated. The goal of this project is to elucidate these mechanisms. ... |
|
| Extracellular Matrix in Breast Cancer Invasion |
JUN 2001 |
67 pages |
| Authors:
Vito Quaranta; SCRIPPS RESEARCH INST LA JOLLA CA
|
| The overall goal of our project is to identify novel treatments for breast cancer invasion and metastasis. We view invasion and metastasis as a breakdown of mechanisms that specify tissue organization. We postulated that metastasis is initiated by molecular cues that improperly stimulate cancer cell motility. Therefore, our approach is to block metastasis by understanding, and then interfering with, molecular and cellular mechanisms that regulate cell motility. We found that, ... |
|
| P270 AND THE SWI/SNF Complex in Breast Cancer |
JUN 2001 |
8 pages |
| Authors:
Xiaomei Wang; Elizabeth Moran; TEMPLE UNIV PHILADELPHIA PA
|
| Breast cancer arises from a series of harmful mutations to genes important for the normal regulation of cell growth and differentiation. Identification of the gene products whose loss is important in the development of the cancer is the primary means of determining who is at risk. There is an acute need for a more comprehensive understanding of the gene products that contribute to regulation, and the consequences of their failures. ... |
|
| Selection and Computational Potential of Gene Control Elements and Their Circuitry |
15 MAY 2001 |
141 pages |
| Authors:
Michael A. Savageau; MICHIGAN UNIV ANN ARBOR DEPT OF MICROBIOLOGY
|
| The physical basis for biological complexity is context-dependent expression of the organism's genome. The context is provided by the life cycle of the organism; the molecular mechanisms of gene regulation interpret that context. We seek to develop a theoretical framework for quantitatively relating the integrated behavior of gene circuits to their underlying molecular determinants, and, by applying this theory to specific classes of gene circuits, we hope to discover the ... |
|
| DNA Replication Arrest and DNA Damage Responses Induced by Alkylating Minor Groove Binders |
MAY 2001 |
53 pages |
| Authors:
Shue-Ru Kuo; Thomas Melendy; STATE UNIV OF NEW YORK AT BUFFALO AMHERST
|
| We are interested in the molecular mechanisms involved in DNA replication arrest by the S phase DNA damage checkpoints. Using in vitro simian virus 40 DNA replication assays, we have found three factors that directly contribute to DNA damage-induced DNA replication arrest: Replication Protein A (RPA), DNA-dependent protein kinase (DNA-PK), and a yet to be identified replication inhibitor. Both DNA-PK and the unknown factor are functioned as trans-acting inhibitors. RPA ... |
|
| Mitosis-Specific Negative Regulation of EGF-Receptor in Breast Cancer: Molecular Mechanisms, Biological Significance and Therapeutic Application |
MAY 2001 |
50 pages |
| Authors:
Stephanie Miller; M D ANDERSON CANCER CENTER HOUSTON TX
|
| The purpose of this project is to study the regulation of EGFR during M-phase. Normally EGFR is negatively regulated during M-phase, however when EGFR is overexpressed it is found to bypass this regulation. We plan to use this negative regulation of EGFR to develop a therapeutic strategy that uses the combination of EGF-PE and taxol as treatment for patients. In trying to understand the mechanism of action of EGFR we ... |
|
| Identification of Cellular and Molecular Markers of Prostate Cancer Progression in Racial-Ethnic Minorities |
MAY 2001 |
13 pages |
| Authors:
Richard J. Cote; UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES
|
| Our specific aims are to examine men with prostate cancer from 4 different racial-ethnic groups to determine the prevalence of molecular and cellular changes that may play a role in prostate tumor progression, particularly invasion and metastasis. We are currently assessing tissue samples from patients with prostate cancer for markers involved in the following pathways: a) hormonal regulation and responsiveness, b) cell cycle regulation c) tumor angiogenesis and its regulators ... |
|
| Molecular Analysis of the Cripto Growth Factor Receptor |
MAY 2001 |
17 pages |
| Authors:
Minjung Kim; Michael M. Shen; UNIVERSITY OF MEDICINE AND DENTISTRY OFNEW JERSEY PISCATAWAY
|
| Work in our laboratory on breast development and tumorigenesis has focused on the Cripto gene, which encodes an extracellular protein that is a member of the EGF-CFC gene family. Several lines of evidence have indicated that Cripto to is involved in autocrine or paracrine signaling during human breast carcinogenesis (reviewed in (Salomon et al. 1999)). In particular, Cripto is overexpressed in a large percentage of human breast cancers, but not ... |
|
| Signaling Mechanisms of Malignant Growth of Prostate Cancer Cells |
APR 2001 |
7 pages |
| Authors:
Anning Lin; CHICAGO UNIV IL
|
| Prostate cancer is the most common malignancy and is the second leading cause of cancer death among males in the United States. Although androgen ablation has been the choice to treat prostate cancer, prostate cancer can recur and proliferate in androgen-deprival environment. Furthermore, androgen-independent prostate cancer cells are refractory to the hormone therapy and resistant to radiation and chemodrugs. Therefore, androgen-independent prostate cancer presents a major direct threat to patient ... |
|
| P16 Axie in Androgen-Dependent Proliferation of Prostate Cancer Cells |
APR 2001 |
7 pages |
| Authors:
Liang Zhu; ALBERT EINSTEIN COLL OF MEDICINE OF (YESHIVA UNIV) BRONX NY
|
| The purpose of this study is to understand the role of the pl6 growth control axis in androgen dependent proliferation of prostate cancer cells. The pl6 axis contains two tumor supressors (pl6Ink4a and pRB), cyclin D-dependent kinases, and transcription factor E2F. Ne hypothesized that functions of the pl6 axis can influence androgen-dependence of prostate cancer cells. To test this hypothesis, we proposed to use controlled expression techniques to determine whether ... |
|
| A Rational Approach to the Design and Synthesis of Tough Glassy Polymers |
20 MAR 2001 |
74 pages |
| Authors:
Albert F. Yee; MICHIGAN UNIV ANN ARBOR
|
| In this work we have designed and synthesized high Tg yet ductile polymers using a building block approach. In this approach functional molecular groups that provide high Tg alternate with molecular linkages that provide mobility. Specifically molecular mobility is provided by in-chain cyclohexylene units. The existence of mobility is verified by dynamic mechanical studies, and supported by positronium annihilation studies. We discovered an unusual behavior in the three series of ... |
|
| Regulation of Androgen Responses in Prostate Cancer by BAG-1 |
MAR 2001 |
55 pages |
| Authors:
John C. Reed; BURNHAM INST LA JOLLA CA
|
| Androgen ablation therapy and anti-androgenic drugs represent the principal treatments for metastatic prostate cancer. However, nearly all tumors eventually relapse as hormone-refractory disease. A need therefore exists for better understanding of the mechanisms that allow prostate cancer cells to grow in an androgen - independent manner. This study focuses on BAG-1, a protein that is expressed in prostate cancers and which binds to androgen receptors, increasing their sensitivity to androgenic ... |
|
| Quantum Theory of Molecular Nanomagnets |
15 FEB 2001 |
4 pages |
| Authors:
Eugene M. Chudnovsky; HERBERT H LEHMAN COLL BRONX NY
|
| The funds have been used entirely to support a CUNY Ph.D. student who has conducted extensive measurements of the magnetic properties of molecular magnets. Most of the attention was directed toward experimentally studying quantum tunneling of magnetism in Mn12-acetate. |
|
| Dynamics in Small Confining Systems V. Symposium Held November 27-30, 2000, Boston, Massachusetts, U.S.A. |
2001 |
394 pages |
| Authors:
J. M. Drake; J. Klafter; Pierre E. Levitz; Rene M. Overney; M. Urbakh; MATERIALS RESEARCH SOCIETY WARRENDALE PA
|
| The sixth symposium on "Dynamics in Small Confining Systems", held November 27-30 at the 2000 MRS Fall Meeting in Boston, Massachusetts, celebrated a decade of this series. The program of the symposium covered a broad range of topics related to static and dynamic properties of confining systems: probing of confined systems, structure and dynamics of liquids at interfaces, nanorheology and tribology, adsorption, diffusion in pores, polymers and membranes, dielectric relaxation ... |
|
| NMR Studies of Polymer Nanocomposites |
2001 |
6 pages |
| Authors:
Steve G. Greenbaum; HUNTER COLL NEW YORK
|
| Methods developed and refined to characterize ion and molecular transport process in disordered media are described. The primary tool is pulsed field gradient NMR. A static field gradient method was developed which makes possible variable pressure diffusion measurement, and the application to the important fuel cell membrane NAFION constitute the first results of their kind ever published. Standard ambient pressure results for lithium ion conducting polymer electrolytes containing nanoscopic silica ... |
|
| Instrumentation for Chemical Sensing and Computational Applications of Molecularly Linked Networks of Nanoscale Metallic Clusters |
15 DEC 2000 |
4 pages |
| Authors:
R. P. Andres; R. P. Reifenberger; D. B. Janes; PURDUE RESEARCH FOUNDATION LAFAYETTE IN
|
| The instrumentation purchased under this Grant includes: (1) an inert atmosphere glove box, (2) a clean room work station, (3) a variable temperature probe station, and (4) an Omicron UHV STM/AFM. These instruments are critical to the DoD funded research at Purdue involving fabrication and characterization of prototype chemical sensor and nanoscale computational elements involving molecularly linked networks of nanometer scale metallic dusters. |
|
| Do Electrons or Holes Activate Molecular O2 on TiO2 Surfaces? |
30 NOV 2000 |
6 pages |
| Authors:
John T. Yates Jr.; PITTSBURGH UNIV PA DEPT OF CHEMISTRY
|
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Reports by Keyword(s)
MOLECULAR PROPERTIES
