| CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer. Addendum |
Jun-2009 |
22 pages |
| Authors:
Yuichiro Tanaka; Rajvir Dahiya; NORTHERN CALIFORNIA INST FOR RESEARCH AND EDUCATION SAN FRANCISCO
|
 | In the first hypothesis, CYP1B1 expression in Caucasian prostate cell lines were increased in cancerous (DU145 & PC-3) compared to normal (RWPE-1) cells. Analysis of expression in human tissue cDNAs from Caucasians also showed higher levels of CYP1B1 in cancer compared to BPH. CYP1B1 protein was present in both races and though not significant, generally was higher in tumor regions compared to normal adjacent regions for both African-Americans and Whites. ... |
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| The Development of Prostate Palpation Skills Through Simulation Training May Impact Early Detection of Prostate Abnormalities and Early Management |
May-2009 |
18 pages |
| Authors:
Gregory J Gerling; VIRGINIA UNIV CHARLOTTESVILLE
|
| This is our first annual report. Our team has made good progress on our three year grant toward achieving aims. We have 5 journal papers either submitted or with physical artifacts near completion. We have recruited a group of students and have established collaborations with other researchers, in particular to gain access to tissue specimens. We have successfully built and validated a materials characterization procedure, a series of algorithms for ... |
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| Dual-Modality Prostate Imaging with PET and Transrectal Ultrasound |
Apr-2009 |
75 pages |
| Authors:
Jennifer S Huber; CALIFORNIA UNIV BERKELEY LAWRENCE BERKELEY LAB
|
| We developed the hardware and software tools needed for dual Positron Emission Tomography-Transrectal Ultrasound (PET-TRUS) imaging of the prostate. We acquired and modified the necessary TRUS equipment to work when mounted on a patient table in conjunction with the prostate-optimized PET scanner or the EXACT HR PET scanner. We developed software to: determine the location of two point sources and calculate the corresponding location of the TRUS probe tip (both ... |
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| Real-Time Tracking of Implanted Markers During Radiation Treatment by Use of Simultaneous kV and MV Imaging |
Mar-2009 |
70 pages |
| Authors:
Rodney D Wiersma; STANFORD UNIV CA SCHOOL OF MEDICINE
|
| In the presence of organ motion, geometric target uncertainty can hamper the benefits of highly conformal dose techniques such as IMRT. A critical step in dealing with intra-fraction prostate tumor motion is the real-time monitoring of the tumor position. The aim of this investigation is the first time demonstration of a real-time 3D internal fiducial tracking system based on onboard kV diagnostic imaging together with a MV electronic portal-imaging device ... |
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| Mechanisms of KAI1/CD82 - Induced Prostate Cancer Metastasis |
Feb-2009 |
28 pages |
| Authors:
Cynthia Miranti; VAN ANDEL RESEARCH INSTITUTE GRAND RAPIDS MI
|
| Our basic understanding of how prostate cancer metastasis develops is limited. The recent identification of genes, whose expression suppresses metastasis but not growth in xenograft models, has provided a potential avenue for better understanding the metastatic process. The overall objective of this proposal is to determine how loss of the metastasis suppressor, KAI1/CD82, promotes the development of metastatic prostate cancer. Elevated expression of integrins a6b1 and a3b1 is highly correlative ... |
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| Involvement of Novel Multifunction Steroid Hormone Receptor Coactivator, E6-Associated Protein, in Prostate Gland Tumorigenesis |
Jan-2009 |
18 pages |
| Authors:
Sathish Srinivasan; MIAMI UNIV FL
|
| E3 ubiquitin-protein ligase enzyme, E6-associated protein (E6-AP), is a novel dual function steroid hormone receptor coactivator. Previously I have shown that E6-AP regulates PI3K-Akt pathway in the prostate gland and as well as LNCaP cells. In this report I have provided evidence that E6-AP plays a vital role in the prostate gland growth and prostate cancer cell proliferation. E6-AP by itself can modulate p53 levels in prostate cancer cells independent ... |
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| Robotic Prostate Biopsy in Closed MRI Scanner |
FEB 2008 |
68 pages |
| Authors:
Gregory Fischer; JOHNS HOPKINS UNIV BALTIMORE MD
|
| MRI possess many of the capabilities that TRUS is lacking for prostate brachytherapy and biopsy, with high sensitivity for detecting prostate tumors, high spatial resolution, excellent soft tissue contrast, and volumetric imaging capabilities. However, closed-bore high-field MRI has not been widely adopted for prostate interventions because strong magnetic fields and confined physical space present formidable challenges. This work enables prostate brachytherapy and biopsy procedures in standard high-field diagnostic MRI scanners ... |
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| Involvement of Novel Multifunctional Steroid Hormone Receptor Coactivator, E6-Associated Protein, in Prostate Gland Tumorigenesis |
JAN 2008 |
20 pages |
| Authors:
Sathish Srinvasan; MIAMI UNIV FL SCHOOL OF MEDICINE
|
| E3 ubiquitin-protein ligase enzyme, E6-associated protein (E6-AP), is a novel dual function steroid hormone receptor coactivator. E6-AP not only interacts with and enhances the hormonedependent transcriptional activities of various steroid hormone receptors, including androgen receptor (AR), but also is a member of the E3 class of functionally related ubiquitin-protein ligases. Previously, using E6-AP knockout animals we have shown that E6-AP is required for the proper development and growth of prostate ... |
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| Non-Invasive Markers of Tumor Growth, Metastases, and Sensitivity to Anti- Neoplastic Therapy |
JAN 2008 |
16 pages |
| Authors:
Jason A. Koutcher; SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK
|
| The goals of this application are to develop methods to non-invasively differentiate fast and slow growing prostate tumors and also develop methods to evaluate response to anti-angiogenic agents. Validation of the results will be based on tumor growth metastases and microvessel density measurement (antiangiogenic studies). To date we have succeeded in demonstrating that the R3327AT rat prostate tumor which is relatively radiation resistant has detectable lactate which is heterogeneously distributed ... |
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| A Role for TACI in Prostate Neoplasia |
Jan-2008 |
5 pages |
| Authors:
Gotz-Ullrich VonBulow; INDIANA UNIV INDIANAPOLIS
|
| This research study proposed that TNF- family growth factors, BAFF and APRIL play a role in prostate cancer. Our hypothesis proposed that APRIL provides a proliferative signal to normal prostate epithelial cells by means of an unknown receptor. We postulated that TACI is an antagonist receptor which functions to regulate APRIL-mediated proliferation of cells by inducing apoptosis and thereby maintaining the normal glandular structure. The loss of TACI expression in ... |
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| Prostate Derived Ets Factor, An Immunogenic Breast Cancer Antigen |
SEP 2007 |
9 pages |
| Authors:
Ashwani Sood; ROSWELL PARK CANCER INST BUFFALO NY
|
| The goal of the proposed research was to test the following concept: Due to the prostate restricted expression of Pse in normal tissues of mice Pse is likely to be immunogenic in female mice. T cell responses of male and female mice to Pse and a control antigen Her2lneu were compared. Two different assays were used for this purpose; the ELlspot assay and the Cytotoxicity assay. We found that in ... |
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| p63 in Development and Maintenance of the Prostate Epithelium |
MAR 2007 |
13 pages |
| Authors:
Sabina Signoretti; BRIGHAM AND WOMEN'S HOSPITAL BOSTON MA
|
| The purpose of this project is to define the role of p63 in the development and maintenance of the prostate epithelium by utilizing both in vivo and in vitro models. In our first year of work, we have the successful constructed the targeting vector for the generation of the p63-Cre-ERT2 knock-in mice to be used for genetic lineage tracing. The p63-Cre-ERT2 vector has been transferred to the DFCI Gene Targeting ... |
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| Transurethral Ultrasound Diffraction Tomography |
MAR 2007 |
48 pages |
| Authors:
Matthias C. Schabel; UTAH UNIV SALT LAKE CITY
|
| The potential for cost-effective tomographic imaging using ultrasound continues to be confronted with difficulties arising from the computational complexity of fully three-dimensional object reconstruction in the diffraction regime. Development of fast and accurate forward and inverse models for ultrasound propagation in the biomedical frequency range of 1-10 MHz is essential for diffraction tomography to be a practical imaging modality. We have implemented a flexible, object-oriented simulation system in MATLAB for ... |
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| BTG2 Antiproliferative Gene and Prostate Cancer |
FEB 2007 |
8 pages |
| Authors:
Paul D. Walden; NEW YORK UNIV NY SCHOOL OF MEDICINE
|
| Levels of the BTG2 tumor suppressor protein diminish during the transition of normal prostate epithelial cells into prostate cancer cells and restoration of BTG2 expression in prostate cancer cells significantly reduces cell proliferation and tumorigenicity. Our working hypothesis being tested is that the tumor suppressive activity of BTG2 is diminished as an early event in prostate carcinogenesis due to increased proteasomal degradation, leading to compromised cell cycle regulation, increased cell ... |
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| Interfering with DNA Damage Signals: Radiosensitizing Prostate Cancer using Small Peptides |
NOV 2006 |
6 pages |
| Authors:
Bo Xu; LOUISIANA STATE UNIV NEW ORLEANS
|
| Our focus of this project is to characterize a newly developed small peptide on its ability to function as a powerful radiosensitizer. Radio sensitivity is mainly controlled by a kinase named ATM and its phosphorylation of downstream targets, including Structural Maintenance of Chromosomal protein on (SMC1). Previously we have demonstrated that small fusion peptides containing SMC1 phosphorylation sequences can inhibit ATM activity. We have characterized the inhibitory effect of the ... |
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| Prostate Dose Escalation by Innovative Inverse Planning-Driven IMRT |
NOV 2006 |
201 pages |
| Authors:
Lei Xing; STANFORD UNIV CA
|
| The goal of this project is to develop innovative inverse treatment planning techniques for prostate radiation therapy. In the last funding period significant progress has been made toward the goal of the project. We have established the voxel-based dose optmization framework for lMRT treatment planning. Realizing that current radiation therapy does not adapt to inter-fraction organ movement and dosimetric errors caused by inaccurate patient setup or organ deformation during a ... |
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| Identification and Validation of PTEN Complex, Associated Proteins |
NOV 2006 |
18 pages |
| Authors:
Rosalia Rabinovsky; DANA-FARBER CANCER INST BOSTON MA
|
| The PTEN/MMAC1/TEP1 is a tumor suppressor gene, targeted for biallelic somatic inactivation in a variety of cancers including advanced prostate adenocarcinomas among many others malignancies. PTEN is a phosphatase and has an important role in regulation of the PI3K/AKT signaling pathway, which plays a key role in regulating cellular functions including proliferation, apoptosis, glucose homeostasis, cell size, nutrient response and DNA damage. Furthermore, PTEN functions in the cell to restrict ... |
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| Prostate Cell Specific Regulation of Androgen Receptor Phosphorylation In Vivo |
NOV 2006 |
7 pages |
| Authors:
Samir Taneja; Susan Logan; Brian Dynlacht; Michael Garabedian; Derick Mitchell; NEW YORK UNIV SCHOOL OF MEDICINE NY
|
| On the basis of previous studies in which we analyze AR phosphorylation in vivo we propose that AR phosphorylation at serines 213 and 650 regulate differential target gene expression and recruitment to gene promoters via altered interaction with other cellular transcription factors. To test this hypothesis we have conducted yeast two-hybrid analysis with the N-terminus of wild type AR as well as AR S213A and AR S213E variants. Our preliminary ... |
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| Prostate Derived Ets Factor, an Immunogenic Breast Cancer Antigen |
SEP 2006 |
6 pages |
| Authors:
Ashwani Sood; HEALTH RESEARCH INC BUFFALO NY
|
| The goal of the proposed research was to test the following concept. Due to prostate restricted expression of Pse in normal tissues of mice, Pse is likely to be immunogenic in female mice. T cell responses of male and female mice to Pse and a control antigen Her-2/neu were compared. Two different assays were used for this purpose; the ELISPOT assay and the Cytotoxicity assay. To date, we have found ... |
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| FGF Signaling and Dietary Factors in the Prostate |
SEP 2006 |
131 pages |
| Authors:
Fen Wang; TEXAS A AND M UNIV COLLEGE STATION HEALTH SCIENCE CENTER
|
| Purpose: To study the FGF signaling axis in prostate homeostasis and tumorigenesis, to evaluate dietary factors in modulating FGF signals in the prostate. Scope: To develop mouse models resembling human prostate tumor progressions for screening therapeutic strategies for prostate cancers and evaluating dietary factors in prostate cancer prevention. Major Finding: Ectopic expression of the constitutively-active FGFR1 (caFGFR1) in the prostate induces high-grade prostatic intraepithelial neoplasia (PIN) in transgenic mice in ... |
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| Prognostic Value of Telomere DNA Content in Ductal Carcinoma in Situ |
JUN 2006 |
9 pages |
| Authors:
Colleen A. Fordyce; CALIFORNIA UNIV SAN FRANCISCO
|
| Critically shortened telomeres cause genomic instability both in vivo and in vitro and thus drives changes in gene expression Reduced telomere DNA content (TC) is associated with reduced survival in breast and prostate cancers We hypothesized that TC could be a unique prognostic marker in DCIS The goals of this project were: I) determine if TC can be used to predict outcome in a retrospective study of DCIS, 2) determine ... |
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| Role of IKKs and Transcription Factor NF-kB in Prostate Tumorigenesis |
MAY 2006 |
71 pages |
| Authors:
Irina V. Budunova; NORTHWESTERN UNIV CHICAGO IL
|
| One of the central anti-apoptotic pathways in cells is mediated by NF-kB (nuclear factor kappa-B) transcription factors. There is mounting experimental evidence that NF- kB is activated during tumorigenesis in different tissues, and that NF-kB is critically important for cell protection against apoptosis induced by different treatments including chemotherapeutic drugs and gamma-irradiation. The key to NF-kB regulation is the inhibitory kB (IkB) proteins which retain NF-kB in an inactive form ... |
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| Prostate Cancer Risk in Relation to IGF-1 and its Genetic Determinants: A Case Control Study Within the Cancer Prostate Sweden Project (CAPS) |
MAY 2006 |
11 pages |
| Authors:
Rudolf J. Kaaks; INTERNATIONAL AGENCY FOR RESEARCH ON CANCER LYONS (FRANCE)
|
| Purpose and scope: A large genetic association study is being conducted to examine relationships of prostate cancer risk with polymorphic variation in a series of selected candidate genes that are involved in pathways determining the synthesis of IGF-I and IGF-binding proteins as well as biological response to IGF-I. The study is being performed within a large Swedish case-control study ("CAPS"). Progress report: We have distributed the selected DNA, selected haplotype ... |
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| PTEN Regulates Beta-Catenin in Androgen Signaling: Implication in Prostate Cancer Progression |
MAR 2006 |
33 pages |
| Authors:
Zijie Sun; LELAND STANFORD JUNIOR UNIV STANFORD CA
|
| The androgen-signaling pathway is essential in male sexual development and in normal and malignant prostate cell growth and survival. PI3K/Akt plays a critical role in prostate cancer cell growth and survival. Recent studies demonstrate that the effect of PI3K/Akt in prostate cells is mediated through androgen signaling. The PI3K inhibitor, LY294002, and a tumor suppressor, PTEN, negatively regulate the PI3K/Akt pathway and repress the androgen receptor (AR) activity. However, the ... |
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| Cannabinoid Receptors: A Novel Target for Treating Prostate Cancer |
01 FEB 2006 |
20 pages |
| Authors:
Hasan Mukhtar; Farrukh Afaq; Sami Sarfaraz; WISCONSIN UNIV-MADISON
|
| Recently we have shown that expression levels of both cannabinoid receptors CB and CB12 are higher in human prostate cancer cells than in normal prostate epithelial cells and treatment of LNCaP cells with WIN-55,212-2 (a mixed CB1/CB2 agonist) resulted in inhibition of cell growth and induction of apoptosis. Based on these data we suggested that WlN-55,212-2 or other non-habit forming cannabinoid receptor agonists could be developed as novel therapeutic agents ... |
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| BTG2 Antiproliferative Gene and Prostate Cancer |
FEB 2006 |
9 pages |
| Authors:
Paul D. Walden; NEW YORK UNIV SCHOOL OF MEDICINE NY
|
| Expression of the protein product of the BTG2 tumor suppressor is lost during the transition of normal prostate epithelial cells into prostate cancer cells. Restoration of BTG2 expression in BTG2 null prostate cancer cells significantly reduces cell proliferation and tumorigenicity. Steady state levels of BTG2 protein are regulated post-translationally in p53 positive prostate cells. Our working hypothesis being tested as part of our proposed studies is that the tumor suppressive ... |
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| HOXC5 as a Biomarker of Disease Presence in Tumor-Associated Normal Prostate |
DEC 2005 |
7 pages |
| Authors:
Steven K. Nordeen; COLORADO UNIV HEALTH SCIENCES CENTER AURORA CO
|
| Our preliminary data had suggested that HOXC5 gene expression was increased in prostate tumor and in tumor-associated normal epithelium compared to epithelium from normal prostate, raising hopes that HOXC5 could be a biomarker that could guide whether a patient with a negative biopsy should undergo a rebiopsy. We compared methods for preparation of RNA from formalin-fixed, paraffin embedded tissue and used the best method to prepare RNA from normal prostate ... |
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| Function of an Androgen Receptor Coactivator Regulated in Prostate Development and Prostate Cancer |
NOV 2005 |
11 pages |
| Authors:
Susan K. Logan; Samir Taneja; Michael Garabedian; Brian Dynlacht; Peng Lee; Susan Ha; NEW YORK UNIV NY SCHOOL OF MEDICINE
|
| We hypothesize that ART-27 affects AR-dependent differentiation of prostate epithelial cells by regulating a subset of AR responsive genes important to prostate growth suppression and differentiation. We further hypothesize that alterations in the level of ART-27 modulates AR activity, which, in turn, affects AR-dependent cell growth regulation in vivo. Our aims are to identify ART-27-dependent AR-target genes involved in growth suppression and differentiation and to elucidate the mechanism of regulation ... |
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| Identification and Validation of PTEN Complex, Associated Proteins |
NOV 2005 |
18 pages |
| Authors:
Rosalia Rabinovsky; DANA-FARBER CANCER INST BOSTON MA
|
| The PTEN/MMAC1/TEP1 is a tumor suppressor gene, targeted for biallelic somatic inactivation in a variety of cancers including advanced prostate adenocarcinomas among many others malignancies. PTEN is a phosphatase and has an important role in regulation of the PI3K/AKT signaling pathway, which plays a key role in regulating cellular functions including proliferation, apoptosis, glucose homeostasis, cell size, nutrient response and DNA damage. Furthermore, PTEN functions in the cell to restrict ... |
|
| Interfering With DNA Damage Signals: Radiosensitizing Prostate Cancer Using Small Peptides |
NOV 2005 |
7 pages |
| Authors:
Bo Xu; LOUISIANA STATE UNIV NEW ORLEANS
|
| The focus of this project is to evaluate a newly developed small peptide on its ability to block DNA damage signaling pathways and to sensitize prostate tumor cells to radiotherapy. One of the critical DNA damage pathways which determine radiosensitivity is medicated by ATM and its phosphorylation of downstream targets, including Structural Maintenance of Chromosomal protein one (SMC1). Previously we have demonstrated that small fusion peptides containing SMC1 phosphorylation sequences ... |
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| SMAD-Mediated Signaling During Prostate Growth and Development |
OCT 2005 |
10 pages |
| Authors:
Charles F. Shuler; Nobuyuki Shiomi; Sharon Stewart; CALIFORNIA UNIV LOS ANGELES
|
| The binding of TGF-Beta family molecules to their receptors on the surfaces of cells initiates a signaling pathway within the cells. The Smad family of molecules mediates the propagation of the intracellular signal from the receptor for TGF-Beta. Two different Smad molecules are essential to propagating this signal, Smad 2 and Smad 3. It has been shown that Smad 2 expression is altered in prostate cancer and adult normal prostate ... |
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| Molecular Profiling of Prostate Cancer to Determine Predictive Markers of Response to Radiation and Receptor Tyrosine Kinase Inhibitor Therapy |
SEP 2005 |
13 pages |
| Authors:
Dong W. Kim; VANDERBILT UNIV MEDICAL CENTER NASHVILLE TN
|
| Ionizing radiation(IR)induces the activation of PI3K/Akt signaling pathway, which in turn regulates endothelial cell viability during treatment with radiotherapy. Inhibition of this pathway by receptor tyrosine kinase inhibitor (TKIs) enhances the cytotoxic effects of radiation in tumor vascular endothelium resulting in improved tumor control. There is significant evidence that multiple receptor tyrosinse kinases may be aberrantly activated in the prostate cancer cells. Therefore, we sought to study the effects of ... |
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| Multimodality Image-Guided HDR/IMRT in Prostate Cancer: Combined Molecular Targeting Using Nanoparticle MR, 3D MRSI, and 11C Acetate PET Imaging |
AUG 2005 |
5 pages |
| Authors:
Karen Kurdziel; Michael Hagan; Jeffrey Williamson; Donna McClish; Panos Fatouros; Jerry Hirsch; Rhonda Hoyle; Kristin Schmidt; Dorin Tudor; Jie Liu; VIRGINIA COMMONWEALTH UNIV RICHMOND
|
| The purpose of the award was to provide funding for the development of a multimodality prostate cancer clinical trial. A clinical protocol was developed along with a supporting infrastructure to begin the trial. A submission for funding under the DOD Prostate Cancer Clinical Trial Award mechanism; however the project was not funded. Submission under an alternative funding mechanism is pending collection of more preliminary data and optimization of C-11 acetate ... |
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| Constitutive Activation of NF-KB in Prostate Carcinoma Cells Through a Positive Feedback Loop: Implication of Inducible IKK-Related Kinase (IKKi) |
AUG 2005 |
60 pages |
| Authors:
Irina V. Budunova; NORTHWESTERN UNIV EVANSTON IL
|
| The overall goal of this project is to understand the role of inducible IKK-related kinase IKKi in constitutive activation of anti-apoptotic transcription factor NF-KB prostate carcinoma (PC) cells. During FYO2 we developed the conditions for RNA isolation from OCT-embedded frozen PC and BPH samples, developed conditions for cell lysis and IKKi immunoprecipitation from transfected cells using FLAG antibody. We also generated PC cell lines stably infected with lentiviruses harboring w.t. ... |
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| Genetic Epidemiology of Prostate Cancer |
JUN 2005 |
15 pages |
| Authors:
Susan L. Neuhausen; CALIFORNIA UNIV IRVINE
|
| Prostate cancer results from complex interactions among genetic, endocrine, and environmental factors. Understanding genetic risk factors that contribute to the occurrence of prostate cancer is crucial to design both preventative and therapeutic strategies and to identify at-risk individuals. This knowledge could reduce the incidence of and death from this disease. The primary objective of this grant is to investigate changes in genes that directly and indirectly regulate levels of male ... |
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| Prostate Cancer Risk in Relation to IGF-I and Its Genetic Determinants: A Case Control Study Within the Cancer Prostate Sweden Project (CAPS) |
MAY 2005 |
9 pages |
| Authors:
Rudolf J. Kaaks; INTERNATIONAL AGENCY FOR RESEARCH ON CANCER LYONS (FRANCE)
|
| A large genetic association study is being conducted, to examine relationships of prostate cancer risk with polymorphic variation in a series of selected candidate genes that are involved in pathways determining the synthesis of IGF-I and IGF-binding proteins, as well as biological response to IGF-l. The study is being performed within a large Swedish case-control study ("CAPS"). |
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| Serum Genetic Markers as Surrogates of Prostate Caner Progression |
APR 2005 |
3 pages |
| Authors:
Dave B. Hoon; JOHN WAYNE INST FOR CANCER TREATMENT AND RESEARCH SANTA MONICA CA
|
| No progress report has been carried out on this project since AMDEX had not approved the human subjects IRB until March 2005. The only work that has been done on this project is multiple revisions and resubmissions of documents pertaining to the human subjects' protocol and consent forms. Therefore this progress report is being submitted as a formality. Recently we submitted a progress report in January 2005 to Dr. Mishra, ... |
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| Functional Erythropoietin Receptors Expressed by Human Prostate Cancer Cells |
APR 2005 |
7 pages |
| Authors:
Arthur J. Sytkowski; BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA
|
| Erythropoietin is effective in correcting the anemia associated with cancer and chemotherapy. However, Epo receptors (EpoR) have been found on tumor cells and Epo may stimulate these cells. We discovered that prostate cancer cell lines and primary prostate tumors express EpoR. In this study, we propose to gain insight into a pattern of EpoR expression in primary human prostate tumors and adjacent normal tissue and to study the role of ... |
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| Microlocalization and Quantitation of Risk Associated Elements in Gleason Graded Prostate Tissue |
MAR 2005 |
12 pages |
| Authors:
Curtis D. Eckhert; CALIFORNIA UNIV LOS ANGELES
|
| There is growing evidence that the elements boron, selenium and zinc reduce PCa risk whereas calcium and cadmium increase risk (1-14). The objective of this proposal is to determine if these elements differ in concentration and location between normal and tumor tissue. During the first year of the project pathologically graded human prostate tissue was analyzed to determine the concentrations of boron, calcium, cadmium, selenium and zinc. There was no ... |
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| Egr1 Target Genes That Regulate Growth/Survival of Prostate Cells |
MAR 2005 |
38 pages |
| Authors:
Jianxiu Yu; BURNHAM INST LA JOLLA CA
|
| Last year I finished the study of the related co-activators p3OO and CBP. I have shown that the immediate early response TF, Egr1, acts upstream of p300/CBP to induce or to repress transcription, depending on the stimulus. I have shown that serum stimulation of prostate cells results in cell growth by the up-regulation of p300/CBP. In contrast, the stimulus of ultraviolet irradiation leads to cell cycle block and apoptosis through ... |
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| PTEN Regulates Beta-Catenin in Androgen Signaling: Implication in Prostate Cancer Progression |
MAR 2005 |
24 pages |
| Authors:
Zijie Sun; STANFORD UNIV CA
|
| The androgen-signaling pathway is important in the growth and progression of prostate cancer. The growth promoting effects of androgen are mediated mostly through the androgen receptor (AR). P13K/Akt plays a critical role in prostate cancer cell growth and survival. It has been shown that the effect of PI3K/Akt in prostate cells is mediated through androgen signaling. The PI3K inhibitor, LY294002, and a tumor suppressor, PTEN, negatively regulate the P13K/Akt pathway ... |
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| Generation of Transgenic Animals Producing Ezymatically Active Prostate- Specific Antigen (PSA) in Normal and Malignant Prostate Tissue |
FEB 2005 |
8 pages |
| Authors:
Samuel R. Denmeade; JOHNS HOPKINS UNIV BALTIMORE MD SCHOOLOF MEDICINE
|
| Prostate-specific antigen (PSA) is used extensively as a serum marker to screen for prostate cancer and is also used as a surrogate marker to assess response to therapy for prostate cancer. The physiologic role of PSA in normal prostate biology is uncertain but the protein appears to play a role in reproduction by enhancing sperm mobility. PSA is a serine protease with chymotrypsin-like specificity. PSA, through it proteolytic activity, may ... |
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| Characterization of a Novel Intracellular Receptor for Phorbol Esters and Diacylglycerol in Prostate Cancer |
FEB 2005 |
10 pages |
| Authors:
Jose L. Martinez; PENNSYLVANIA UNIV PHILADELPHIA
|
| The small GTP-binding protein Rac controls essential functions, including actin cytoskeleton reorganization, cell proliferation, cell cycle progression, adhesion, migration and invasion. The relationship of Rac to prostate carcinogenesis has not been extensively studied. However upstream activators of Rac have been described to be hyperactivated in prostate cancer, and it is well known that growth factors are very important in the control of prostate cancer proliferation and progression, as well as ... |
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| Cannabinoid Receptors: A Novel Target for Therapy of Prostate Cancer |
FEB 2005 |
24 pages |
| Authors:
Hasan Mukhtar; Farrukh Afaq; Sami Sarfaraz; WISCONSIN UNIV-MADISON
|
| Cannabinoids have received renewed interest in recent years due to their diverse pharmacological activities such as cell growth inhibition and tumor regression. Here we show that expression levels of both cannabinoid receptors CB(sub 1) and CB(sub 2) are significantly higher in CA-HPV-10 and other human prostate cells LNCaP, DUI45, PC3, and CWR22RV1 than in human prostate epithelial and PZ-HPV-7 cells. WIN-55,212-2 (mixed CB(sub 1)/CB(SUB 2) agonist) treatment to LNCaP cells ... |
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| Targeting MRS-Defined Dominant Intraprostatic Lesions with Inverse-Planned High Dose Rate Brachytherapy |
FEB 2005 |
58 pages |
| Authors:
Jean Pouliot; I-Chow Hsu; John Kurhanewicz; Sue Noworelski; CALIFORNIA UNIV SAN FRANCISCO
|
| During this first year, we have obtained CHR approval (December 2004) from the three step process (C.U., P.R.C. and C.H.R.) committees at UCSF. Patient enrollment will begin immediately after receiving CHR approval from D.O.D. The accuracy of aligning MRI/MSRI data acquired with different types of endorectal probes to images acquired without a probe was determined. A 94% Average overlap of prostate contours was obtained using rigid endorectal coil. We have ... |
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| Origin and Properties of Prostatic Stem Cells |
FEB 2005 |
9 pages |
| Authors:
Elaine L. Wilson; NEW YORK UNIV TUXEDO SCHOOL OF MEDICINE
|
| The aim of this proposal is to isolate and characterize prostatic epithelial stem cells. This will be done as stem cell and tumor cell biology may be closely linked and stem cells may have a role in the etiology of cancer. Stem cells and tumor cells have many common features including self-renewal, multi- drug resistance, telomerase expression and, in the instance of the prostate, androgen independence. Stem cells from a ... |
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| Gene Targets in Prostate Tumor Cells that Mediate Aberrant Growth and Invasiveness |
FEB 2005 |
31 pages |
| Authors:
Craig A. Hauser; Gabriele Foos; BURNHAM INST LA JOLLA CA
|
| Our hypothesis that the human PPC-1 prostate tumor cell lines with experimentally altered Ets transcription factor function, which show a reduction in the transformed phenotype, do so because of altered expression patterns in important genes downstream of Ets factors. We proposed to analyze global differences in gene expression between these cell lines, and assess the functional significance of changes in gene expression. Altered Ets function was found to delay xenograft ... |
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| Imaging Metastatic Prostate Cancer After Genetic Manipulation of Transcriptional Memory Regulators EZH2 and EED |
JAN 2005 |
6 pages |
| Authors:
Lily Wu; CALIFORNIA UNIV LOS ANGELES
|
| This project is intended to explore the role of polycomb group protein EZH2 and its binding partner EED in metastatic prostate cancer. Both proteins will be introduced and overexpressed independently overexpressed in prostate cancer xenograft models by lentiviral transduction. Concordantly, the effects of reduced EZH2 or EED expression will be evaluated by RNAi technology. The growth and progression of the tumors will be monitored by optical imaging. The hypothesis is ... |
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| Molecular Epidemiology of Prostate Cancer |
JAN 2005 |
17 pages |
| Authors:
Bruce J. Trock; JOHNS HOPKINS UNIV BALTIMORE MD
|
| This is the Final Report for this project. It provides new updated information since the last report, which was submitted in June 2004, and provides an overall summary of progress, and remaining steps required to meet the study objectives. Although those objectives were not completed during the funding period, they will be completed using other funding sources. The objective of this case-control study is to determine whether oxidative damage is ... |
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| Lysophosphatidic Acid Regulation and Roles in Human Prostate Cancer |
JAN 2005 |
68 pages |
| Authors:
Sarah Spiegel; VIRGINIA COMMONWEALTH UNIV RICHMOND
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| Lysophosphatidic acid (LPA) is a major mitogen in serum that regulates an array of cellular processes related to pathogensis of cancer, especially ovarian, prostate and breast cancer. Interest in LPA has accelerated recently with the discovery that it is a ligand of a family of three G protein coupled cell surface receptors. Prostate cancer cells express these LPA receptors and it has been suggested that their expression correlates with more ... |
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*PROSTATE GLAND
