| Real-time PCR and PCR-tandem Mass Spectrometry for Biodetection |
01 OCT 2005 |
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| Authors:
Alvin Fox; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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 | Briefing compares real-time polymerase chain reaction (PCR) to polymerase chain reaction/mass spectrometry (PCR-MS) and tandem mass spectrometry (MS-MS). |
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| Novel Approaches for Targeting Antiviral Agents in the Treatment of Arena-, Bunya-, Flavi-, and Retroviral Infections |
01 AUG 91 |
186 pages |
| Authors:
J. D. Gangemi; Abdul Ghaffar; Eugene P. Mayer; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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 | SUMMARY YEAR 1 - The first year of this contract was devoted to the conjugation of selected antivirals (ribavirin and PMEA) immunomodulators (MDP) to neoglycoprotein carriers (poly-L-lysine or BSA). our in vivo virus challenge studies suggest that MDP-BSA and Poly-L-lysine conjugates were more effective than free MDP in enhancing resistance to HSV-1 hepatitis and pneumonitis. Moreover, conjugated MDP was more effective in enhancing RES function than was free drug. In ... |
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| Novel Approaches for Targeting Antiviral Agents in the Treatment of Arena-, Bunya-, Flavi-, and Retroviral |
JUN 90 |
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| Authors:
J. D. Gangemi; Abdul Ghaffar; Eugene P. Mayer; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| Administration of multiple doses of Poly I:C-LC, Riker-3M or CL 246878 every other day for ten days did not prolong activation of immune functions. In some cases this treatment actually suppressed immune functions. Nevertheless, administration of multiple doses of these drugs had no adverse effect on resistance to Banzi virus infection. Encapsulation of Poly I:C in liposomes resulted in a formulation which was more effective than free drug in augmenting ... |
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| Biology of Immunomodulators |
MAY 90 |
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| Authors:
Abdul Ghaffar; J. D. Gangemi; Eugene P. Mayer; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| During this contract period we have examined approximately 25 immunomodulating drugs for their ability to stimulate various components of the specific and nonspecific immune systems as well as their ability to enhance resistance to influenza, herpes-, arena-, bunya- and flaviviruses. All of these compounds were able to stimulate at least one component of the immune system and some were able to stimulate many. Although there was no correlation between stimulation ... |
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| Biology of Immunomodulators |
JUL 88 |
111 pages |
| Authors:
Abdul Ghaffar; J. D. Gangemi; Eugene P. Mayer; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| A number of pyrimidinones were compared for their effects on the reticuleondothelial system (RES) function, macrophage cytotoxicity, prostaglandin secretion, serum interferon levels and resistance to herpes, Aichi and Banzi virus infections. Five of these agents (ABPP, ACPP, AIPP, ABMFPP and ACDFPP) stimulated the RES function when tested two days after treatment. However, their effects were not as pronounced four days post treatment. They also caused a reduction in prostaglandin secretion ... |
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| Selective Targeting of Antiviral and Immunomodulating Agents in the Treatment of Arenavirus Infections |
01 OCT 87 |
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| Authors:
J. D. Gangemi; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| Large multilamellar liposomes were used as a delivery vehicle for the antiviral, ribavirin, and the macrophage activator, muramyl tripeptide (MTP-PEO. The data presented confirms previous observations on the altered tissue (RES) and cellular (macrophage) disposition of intravenously administered drugs and provides evidence to support the therapeutic potential of liposomes in therapy of arenavirus, bunyvirus, orthomyxovirus and herpesvirus infections. Encapsulation of MTP-PE into liposomes enhanced this drug's ability to activate peritoneal, ... |
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| Biology of Immunomodulators |
JUL 87 |
54 pages |
| Authors:
J. D. Gangemi; Abdul Ghaffar; Eugene P. Mayer; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| We have compared effects of Poly I:C-LC, CL 24673 and Ampligen on clearance and organ localization of radiolabelled sheep erytorocytes (SRBC); peritoneal, splenic and liver cell phagocytosis; antibody PFC responses; splenic and liver natural killer (NK) cytotoxicity; specific T cell cytotoxicity; enumeration of lymphoid cell subpopulations; macrophage antiviral activity and serum interferon levels. We have also examined the effect of these agents on resistance to herpes, Punta Toro and Banzi ... |
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| Selective Targeting of Antiviral and Immunomodulating Agents in the Treatment of Arenavirus Infections |
30 JUN 86 |
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| Authors:
J. D. Gangemi; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| The studies presented in this report further document the ability of liposome-encapsulation to augment the immunopotentiating and therapeutic properties of the synthetic immunomodulator, muramyl tripeptide (MTP-PE). Encapsulation of MTP-PE into large negatively charged liposomes enhanced this drugs ability to activate peritoneal, alveolar, and liver macrophage antiviral functions. Moreover, liposome-encapsulation of MTP-PE reduced virus replication in target organs and provided better protection in murine models of herpes simplex type 1 (HSV-1) ... |
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| The Effect of Chemotherapeutic Agents on Immune Reactions |
AUG 1982 |
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| Authors:
A. Ghaffar; SOUTH CAROLINA UNIV COLUMBIA SCHOOL OF MEDICINE
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| Immunotoxic effects of WR 99,210, WR 122,455, and WR-228,258 were tested in the animal model for the screening. WR 99,210 caused a significant enhancement in the phagocytic function (alpha value), and severe depression in the antibody PFC response when given before antigen, and reduction in the liver and spleen sizes. WR 122,455 and WR 228,258 had similar effects, although with somewhat less severity. Thus, the reduction in the PFC responses ... |
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Reports by Corporate Author
