| Fluid Settlement Profiler: Error Analysis For Installation at Blackstrap, Saskatchewan |
MAY 1979 |
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| Authors:
S. S. Tao; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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 | The accuracy of settlement measurement at the base of a man-made ski hill, using a fluid settlement profiler, has been analysed. Owing to the great length of the installation, changes in ambient temperture and barometric pressure contributed most to overall error. Other factors, such as probe positioning, level reference to benchmark, and transducer system reproducibility also affected accuracy. At the centre of the settlement profile the possible error was found ... |
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| Factors Affecting the Isolation and Callus Formation in Protoplasts from the Shoot Apices of Pisum sativum L, |
26 DEC 1974 |
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| Authors:
Oluf L. Gamborg; J. Shyluk; K. K. Kartha; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| A Comparative Study of Sugars and Sugar Alcohols on Cell Regeneration and Sustained Cell Division in Plant Protoplasts, |
27 NOV 1974 |
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| Authors:
M. R. Michayluk; K. N. Kao; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Chemosystematic Studies of the Volatile Oils of Juniperus Horizontalis, J. Scopulorum and J. Virginiana, |
30 SEP 1974 |
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| Authors:
Ernst Von Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Volatile Leaf Oil Analysis in Chemosystematic Studies of North American Conifers, |
04 FEB 1974 |
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| Authors:
Ernst Von Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Regeneration of Pea ('Pisum sativum L'.) Plants from Shoot Apical Meristems, |
23 OCT 1973 |
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| Authors:
K. K. Kartha; O. L. Gamborg; F. Constabel; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| A procedure has been developed to obtain complete plants from meristems of three cultivars of Pisum sativum L. Benzyladenine (BA) alone or in combination with naphthaleneacetic acid (NAA) at molar concentrations of 5 x .0000005 and .000001 respectively, induced shoot differentiation in meristems cultured on B5 medium at 26C, 60% relative humidity and exposed to fluorescent light (4000 lux, photoperiod 18 hours). When applied alone, NAA induced complete plant formation. ... |
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| Regeneration of Cassava Plants from Apical Meristems, |
04 SEP 1973 |
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| Authors:
K. K. Kartha; O. L. Gamborg; F. Constabel; J. P. Shyluk; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Apical meristem culture offers a rapid, efficient method for vegetative propagation of plants and for eliminating systemic viral infections. Since the first demonstration that virus-free dahlia plants could be regenerated from virus-infected plants by culturing apical meristems, this technique has been utilised to eliminate viruses from a wide range of plant species. Cassave (Manihot utilissima Pohl.) belonging to the family Euphorbiaceae is propagated vegetatively from stem cuttings. The crop is ... |
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| Leaf Wax of 'Triticum aestivum', |
29 MAR 1973 |
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| Authors:
Alexander P. Tulloch; Leslie L. Hoffman; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Leaf waxes of a club wheat and of two durum wheats were analysed previously. The wheat varieties most commonly grown in Canada, However, are varieties of Triticum aestivum L. (Gramineae) having the spring growth habit. Accordingly, in continuation of an investigation of differences between waxes of species and varieties of plants important in agriculture, the composition of the waxes of two varieties of spring wheat have been determined. The varieties ... |
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| Composition of Leaf Surface Waxes of 'Triticum' Species: Variation with Age and Tissue, |
29 MAR 1973 |
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| Authors:
Alexander P. Tulloch; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The composition of cuticular wax from plants of spring wheat (varieties Selkirk and Manitou) and of durum wheat (variety Stewart 63) at various stages of growth, and of wax from different parts of the plants varies considerably. Wax was analysed, without preliminary separation, by GLC using Dexsil 300 as liquid phase. Alcohols are major components of wax from leaf blades and beta-diketones are major components of wax from leaf sheaths, ... |
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| Coumarins and Chromones from 'Lomatium macrocarpum', |
19 MAR 1973 |
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| Authors:
Warren Steck; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Roots of Lomatium macrocarpum (Hook. and Arn.)C. and R. yielded osthol(7-methoxy-8-(3-methyl-2-butenyl)-coumarin) and a chromone, 2-methyl-5-hydroxy-6(3-methyl-2-butenyl)-7-methoxychromone, identified spectroscopically and by synthesis. The serial parts of the plant also contained this chromone along with sibiricin (5,7-dimethoxy-8-(3-methyl-2,3-epoxybutyl)-coumarin) and a new coumarin named macrocarpin. By spectroscopy and chemical degradation macrocarpin was shown to be 7-methoxy-8-(3-methyl-4-(2-methyl-cis-2-butenoyloxy)-cis-2-butenyl)coumarin. These products were not found in four other Lomatium species examined. (Author) |
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| Chemosystematic Studies in the Genus 'Pseudotsuga'. III. Population Differences in British Columbia as Determined by Volatile Leaf Oil Analysis, |
28 FEB 1973 |
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| Authors:
E. VON Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Detailed analysis of the volatile leaf oil terpenes of different Douglas-fir populations and provenances growing in British Columbia confirmed the previous classification into the following terpene types: three coastal (A, B, and C), coastal intermediate, intermediate, interior intermediate, and Rocky Mountain. In areas of overlap of the coastal and Rocky Mountain varieties a continuous gradation of terpene types may be encountered. Differences in elevation or soil sites within homogeneous populations ... |
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| Geographical Variation in the Terpene Composition of the Leaf Oil of Douglas Fir, |
1973 |
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| Authors:
E. VON Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The two forms of Douglas fir, Pseudotsuga menziesii var. menziesii and var. glauca, differ quite considerably with regard to the monoterpenes of their leaf oils. Several chemical races, differing quantitatively in certain monoterpenes, appear to exist in each variety and the leaf oil composition seems to be particularly useful in classifying intermediate forms. (Author) |
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| The Position of Phosphate Groups in the Phosphonomannan of 'Hansenula capsulata', as Determined by Carbon-13 Magnetic Resonance Spectroscopy, |
18 DEC 1972 |
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| Authors:
Philip A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The carbon-13 magnetic resonance spectrum of a phosphate of 2-O-beta-D-mannopyranosyl-alpha-beta-D-mannose was compared with that of the unphosphorylated disaccharide. The positions and number of the signals of C-5's which are coupled to phosphorus-31, together with other spectral data show that the phosphate group is on C-6 of the reducing end-unit. This differs from a previous assignment, thus necessitating a revision to the structure proposed for the parent Hansenula capsulata phosphonomannan. (Modified ... |
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| Preparation of glycosides of 2-deoxy-2-methylamino-D-mannose, 2-deoxy-2-methylamino-D-glucose, and 2-deoxy-2-methylamino-D-galactose: Observations on N-methylation of amides. |
06 NOV 1972 |
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| Authors:
P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Benzyl 2-((benzyloxycarbonyl)methylamino)-2-deoxy-alpha-D-mannopyranoside and its furanose isomer, the derived N-methyloxazolidinones, benzyl 2-((benzyloxycarbonyl)methylamino)-2-deoxy-beta-D-glucofuranoside and methyl 2-deoxy-2-methylacetamido-beta-D-galactofuranoside, were prepared from appropriate diethyl dithioacetals. They were considered the most suitable starting materials for synthesis of O-methyl-2-deoxy-2-methylamino-hexoses because of their ease of preparation and the presence of suitable blocking groups. Oxazolidinones were prepared from N-benzyloxycarbonyl derivatives of 2-amino-2-deoxy-D-mannose by using methanolic sodium methoxide. Their use in preparation of 2-deoxy-2-methyl-amino derivatives is discussed. The Kuhn reagent ... |
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| 13C Magnetic Resonance Spectroscopic Evidence for Formation of Borate Complexes of Polyhydroxy Compounds, |
08 OCT 1972 |
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| Authors:
P. A. J. Gorin; M. Mazurek; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Borate complexes, formed on addition of sodium tetraborate to solutions of carbohydrates in D2O, can be detected by monitoring changes in the 13C magnetic resonance spectra of the parent compounds. In one type of change the chemical shifts remain constant, but broadening of signals of 13C atoms in the vicinity of the complex occurs. In the other type, the signals remain sharp, but changes in chemical shift take place. In ... |
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| The Utilization of Aromatic Compounds by Yeasts, |
26 JAN 1971 |
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| Authors:
Cecily Mills; J. J. Child; J. F. T. Spencer; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Yeasts belonging to 6 genera were isolated from sewage and tested for their ability to use 15 hydroxy derivatives of phenol and benzoic acid as carbon source. The majority were able to produce colonies on at least 10 of the simple monophenol derivatives tested. Salicylic acid, p-hydroxybenzoic acid and gentisic acid were the most commonly used benzoic acid derivatives and resorcinol and phloroglucinol the most frequently metabolized phenols. However, there ... |
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| Beeswax: Structure of the Esters and Their Component Hydroxy Acids and Diols, |
1971 |
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| Authors:
A. P. Tulloch; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Beeswax has been separated into hydrocarbons (14%), monoesters (35%), diesters (14%), triesters (3%), hydroxy monoesters (4%), hydroxpolyesters (8%), free acids (12%), acid monoesters (1%), acid polyesters (2%) and unidentified material (7%). The esters have been identified by thin layer chromatography, gas liquid chromatography and nuclear magnetic resonance spectroscopy and by comparison with synthetic mono-, di- and triesters, hydroxy mono- and dieters and acid esters. The compositions of the acids, hydroxy ... |
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| Embryogenesis and Production of Albino Plants from Cell Cultures of 'Bromus inermis', |
10 SEP 1970 |
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| Authors:
Oluf L. Gamborg; F. Constabel; R. A. Miller; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Embryogenesis occurs in cells from brome grass grown in suspension culture in defined medium. Exogenous hormones are not required. The embryos develop into plants which lack chlorophyll. (Author) |
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| Leaf Wax of Durum Wheat, |
19 JUN 1970 |
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| Authors:
A. P. Tulloch; L. L. Hoffman; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Leaf waxes from durum wheat varieties Pelissier and Stewart 63 contained hydrocarbons (9%), esters (9%), alcohols (17%), acids (3%), beta-diketone (36%), hydroxy beta-diketone (9%) and unidentified material (17%). The major hydrocarbon was nonacosane, the C36-C56 esters were esters of C20-C28 alcohols with C16-C30 saturated acids and trans 2-docosenoic and 2-tetracosenoic acids, and the principal alcohol was octacosanol. The beta-diketone was hentriacontane-14,16-dione and the hydroxy beta-diketone was 25(S)-hydroxyhentriacontane-14,16-dione. (Author) |
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| Systematics of the genus Torulopsis: Proton Magnetic Resonance Spectra of the Mannose-Containing Polysaccharides as an Aid in Classification, |
01 JUN 1970 |
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| Authors:
J. F. T. Spencer; P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The proton magnetic resonance spectra of the mannose-containing polysaccharides of 42 Torulopsis species were determined and used as a basis for placing these species in seven groups. Five of the groups consisted of species which formed mannose-containing polysaccharides with spectra whose H-1 regions were like those of the mannans and galactomannans of species of Saccharomyces, Pichia, Hansenula, Debaryomyces or Metschnikowia. The sixth group consisted of Torulopsis bombicola, Torulopsis apicola and ... |
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| PLANT PROTOPLASTS FOR USE IN SOMATIC CELL HYBRIDIZATION, |
16 FEB 1970 |
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| Authors:
W. A. Keller; B. Harvey; O. L. Gamborg; R. A. Miller; D. E. Eveleigh; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Somatic cell hybridization is a potentially useful technique for the introduction of genetic variability into plant species. One proposed method involves fusion of plant protoplasts (which is a cell lacking its wall--defined here on the basis of light microscope examination) to form a hybrid cell which can be induced to form a plant. Plant protoplasts produced by the use of crude commercial lytic enzymes are not always healthy (as defined ... |
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| SPECTROFLUOROMETRIC DETERMINATION OF TETRAHYDRO-6-OXO-3-PYRIDAZINE CARBOXYLIC ACID (PCA) WITH o-NITROBENZALDEHYDE, |
09 FEB 1970 |
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| Authors:
Thomas A. LaRue; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| TORULOPSIS BOMBICOLA SP. N., |
1970 |
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| Authors:
J. F. T. Spencer; P. A. J. Gorin; A. P. Tulloch; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| A new yeast species, Torulopsis bombicola, is described, that produces extracellular hydroxy fatty acid sophorosides. It utilizes relatively few carbon compounds. It forms a mannan having a proton magnetic resonance spectrum similar to the spectra of the galactomannans of Torulopsis apis, Torulopsis nodaensis and T. magnoliae, but differing from those of T. gropengiesseri galactomannan and T. apicola mannan. (Author) |
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| THE ROLE OF MARMESIN AND COLUMBIANETIN IN THE BIOSYNTHESES OF FURANOCOUMARINS, |
25 OCT 1969 |
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| Authors:
Warren Steck; M. El-Dakhakhny; Stewart A. Brown; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Biosynthetic pathways leading to the furanocoumarins, in contrast with those to simple coumarins, are not well understood. To date, no intermediate in a proposed reaction sequence has been identified. The report presents observations strongly indicating participation of two alpha-hydroxyisopropyldihydrofuranocoumarins, marmesin, and columbianetin as natural intermediates in the elaboration of linear and angular furanocoumarins, respectively. (Author) |
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| LOCATION OF ACYL GROUPS ON TWO PARTLY ACYLATED GLYCOLIPIDS FROM STRAINS OF USTILAGO (SMUT FUNGI), |
29 SEP 1969 |
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| Authors:
S. S. Bhattacharjee; R. H. Haskins; P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Partly acylated ustilagic acids 8 (from Ustilago maydis (DC) Corda (= U. zeae Unger) PRL-119), consisting of partially esterified beta-cellobiosyl residues glucosidically linked to long-chain, hydroxylated fatty acids, and a mixture of partially acylated derivatives of 4-O-beta-D-mannopyranosyl-D-erythritol from Ustilago sp. (probably U. nuda (Jens.) Rostr. = U. tritici (Pers.) Rostr.) PRL-627 were acetalated with methyl vinyl ether, deacylated, and methylated. Vigorous acid hydrolysis of a methylated 8 gave 2- and ... |
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| HYDROXYLATION OF OLEIC ACID BY CELL-FREE EXTRACTS OF A SPECIES OF TORULOPSIS, |
25 AUG 1969 |
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| Authors:
E. Heinz; A. P. Tulloch; J. F. T. Spencer; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The '12000 x g supernatant fraction' from extracts of cells of a yeast, a species of Torulopsis, converts oleic acid to 17-L-hydroxyoleic acid. The hydroxylating system is apparently a mixed-function oxidase since it requires O2 and NADPH. Enzymatic activity is maximal close to pH 7.1, and can be sedimented at 48000 x g. It is strongly inhibited by CO but not by CN(-) or N3(-). |
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| THE INFLUENCE OF CARBON AND NITROGEN NUTRITION ON GROWTH AND STEROL-INDUCED SEXUALITY OF PYTHIUM SP. PRL 2142, |
06 AUG 1969 |
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| Authors:
J. J. Child; G. Defago; R. H. Haskins; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The influence of the major constituents of a chemically defined nutritional medium on growth and sexuality of Pythium sp. PRL 2142 has been determined. Addition of cholesterol to the culture medium stimulated vegetative growth and the response to sterol was quantitative up to a maximum concentration of 60 mg of sterol per liter. The number of oospores produced was proportional to the concentration of sterol within limits determined by the ... |
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| SYSTEMATICS OF THE GENERA SACCHAROMYCES, SCHIZOSACCHAROMYCES, ENDOMYCOPSIS, KLUYVEROMYCES, SCHWANNIOMYCES AND BRETTANOMYCES: PROTON MAGNETIC RESONANCE SPECTRA OF THE MANNANS AND MANNOSE-CONTAINING POLYSACCHARIDES AS AN AID IN CLASSIFICATION, |
22 JUL 1969 |
|
| Authors:
J. F. T. Spencer; P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The proton magnetic resonance spectra of the mannans and mannose-containing polysaccharides of 71 species of Saccharomyces, Schizosaccharomyces, Endomycopsis, Kluyveromyces, Brettanomyces, Nematospora and Schwanniomyces and of some apparently related species of Torulopsis were determined, grouped according to similarities in the spectra, and compared with those of the mannose-containing polysaccharides of some other yeasts. The spectra of the mannans produced by Saccharomyces, Kluyveromyces, Nematospora, Brettanomyces and Torulopsis were placed in 10 groups. ... |
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| HYDROGENOLYSIS OF CYCLIC AND ACYCLIC ORTHOESTERS OF CARBOHYDRATES WITH LITHIUM ALUMINUM HYDRIDE-ALUMINUM TRICHLORIDE, |
24 JUN 1969 |
|
| Authors:
S. S. Bhattacharjee; P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| Several cyclic and acyclic carbohydrate orthoesters have been hydrogenolyzed with 1:1 lithium aluminum hydride-aluminum trichloride, and the mechanisms of the reaction have been studied. Generally, cyclic orthoesters are cleaved via cyclic carbonium-ion intermediates that are stereospecifically attacked by the reducing species, giving cyclic acetals in good yield. One example is the cleavage of both diastereoisomers of 1,2-O-(1-methoxyethylidene)-3,4,6-tri-O-methyl-beta-D-mannopyranose to 1,2-O-ethylidene-3,4,6-tri-O-methyl-beta-D-mannopyranose having an exo O-ethylidene proton. Cleavage of carbohydrate acyclic orthoesters with ... |
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| ENZYMIC DEGRADATION OF YEAST CELL-WALL MANNANS AND GALACTOMANNANS TO POLYMERIC FRAGMENTS CONTAINING alpha-(1-6)-LINKED D-MANNOPYRANOSE RESIDUES, |
06 MAY 1969 |
|
| Authors:
P. A. J. Gorin; J. F. T. Spencer; D. E. Eveleigh; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The cell-wall D-mannans of Candida parapsilosis, Endomycopsis fibuliger, Saccharomyces rouxii, Torulopsis apicola (Hajsig strain), and Torulopsis bombi were degraded with an exo alpha-D-mannosidase from Arthrobacter GJM-1 to their alpha-(1-6)-linked D-mannopyranose main-chains, as demonstrated by p.m.r. spectroscopy. D-Galacto-D-mannans from Candida lipolytica, Torulopsis gropengiesseri, Torulopsis lactis-condensi, Torulopsis magnoliae, and Trichosporon fermentans could be degraded to polysaccharides containing mainly 6-O-linked alpha-D-mannopyranosyl residues following preferential removal of their enzyme-resistant, D-galactopyranosyl non-reducing end-units with acid. ... |
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| REINVESTIGATION OF THE STRUCTURE OF ACYL GALACTOSYL DIGLYCERIDE FROM SPINACH LEAVES, |
14 FEB 1969 |
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| Authors:
Ernst Heinz; Alexander P. Tulloch; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| Shown by nuclear magnetic resonance spectroscopy and by synthesis is that the acyl galactosyl diglyceride from spinach leaves is 1',2'-di-O-acyl-3'-O-(6-O-acyl-beta-D-galactopyranosyl)-sn-glycerol and not a mixture of isomers as reported previously. (Author) |
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| A PROTON MAGNETIC RESONANCE STUDY OF THE ANOMERIC SPECIES PRODUCED BY d-GLUCOSIDASES, |
07 OCT 1968 |
|
| Authors:
D. E. Eveleigh; A. S. Perlin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The anomeric configuration of sugars released during enzymic hydrolysis of some D-glucosides and D-glucans in deuterium oxide has been determined by proton magnetic resonance spectroscopy. In some instances, the data are more highly definitive than those provided by methods previously used. The overall results indicate that D-glucosidases and polysaccharases of the endo type yield products in which there is retention of the anomeric configuration of the substrate, whereas polysaccharases of ... |
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| ETHYLENE PRODUCED BY PLANT CELLS IN SUSPENSION CULTURES, |
28 AUG 1968 |
|
| Authors:
O. L. Gamborg; T. A. G. LaRue; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The formation of ethylene by plants is increased by the application of growth regulators such as 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA). Plant cell suspension cultures were analyzed for the production of ethylene and have examined the effect of 2,4-D and NAA on the amounts produced. |
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| SYSTEMATICS OF THE GENUS CANDIDA BERKHOUT: PROTON MAGNETIC RESONANCE SPECTRA OF THE MANNANS AND MANNOSE-CONTAINING POLYSACCHARIDES AS AN AID IN CLASSIFICATION, |
05 AUG 1968 |
|
| Authors:
J. F. T. Spencer; P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The proton magnetic resonance spectra of the mannans, galactomannans and mannose-containing heteropolysaccharides of a number of species of Candida were determined, and compared with the spectra of similar polysaccharides of some species of the sporogenous genera Pichia, Hansenula, Endomycopsis, Debaryomyces and Saccharomyces. Similarities among the spectra were used as an aid in classification. The Candida species studied were placed in four groups: (1) those Candida species classified as asporogenous forms ... |
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| METABOLISM OF CINNAMIC ACID IN PLANTS: CHLOROGENIC ACID FORMATION, |
15 MAY 1968 |
|
| Authors:
Warren Steck; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| Rapid, efficient synthesis of chlorogenic acid from cinnamate was observed in leaves of plants of several genera. Use of labelled cinnamate,p-coumarate and caffeate showed two pathways were operative in this synthesis in tobacco. Trapping experiments indicated the major route to be:cinnamic acid to p-coumaric acid to p-coumaroylquinic acid to chlorogenic acid, and the secondary route cinnamic acid to p-coumaric acid to caffeic acid to chlorogenic acid. The metabolism of chlorogenic ... |
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| SYNTHESIS OF 2-AMINO-2-DEOXY-D-TALOSE AND 2-AMINO-2-DEOXY-D-GALACTOSE, |
08 MAR 1968 |
|
| Authors:
M. B. Perry; Ann C. Webb; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| Treatment of D-lyxose with nitromethane in the presence of sodium methoxide gave 1-deoxy-1-nitro-D-galactitol which, after conversion to 2,3,4,5,6-penta-O-acetyl-1-deoxy-1-nitro-D-galactitol, reacted with saturated methanolic ammonia solution to yield 2-acetamido-1,2-dideoxy-1-nitro-D-talitol and 2-acetamido-1,2-dideoxy-1-nitro-D-galactitol. 2-Acetamido-1,2-dideoxy-1-nitro-D-talitol and 2-acetamido-1,2-dideoxy-1-nitro-D-galactitol were converted by a modified Nef reaction to 2-acetamido-2-deoxy-D-talose and 2-acetamido-2-deoxy-D-galactose which on hydrolysis with hydrochloric acid afforded 2-amino-2-deoxy-D-talose hydrochloride and 2-amino-2-deoxy-D-galactose hydrochloride. The properties and derivatives of the aminoglycoses are described. (Author) |
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| SYNTHESIS AND NUCLEAR MAGNETIC RESONANCE SPECTRA OF SOME PARTIALLY ACYLATED BETA-D-GLUCOPYRANOSIDES, |
19 FEB 1968 |
|
| Authors:
A. P. Tulloch; A. Hill; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The synthesis of ten new partially acylated derivatives of methyl beta-D-glucopyranoside, all with an acyl group at C-6, is described. The nuclear magnetic resonance spectra of these compounds, and of a number of related derivatives, have been measured using pyridine, acetone-d6, and deuteriochloroform as solvents. When OH-4 is acylated, the H-6 signals are at higher field (by 0.1-0.3 p.p.m.) than when OH-4 is not acylated, but this effect is not ... |
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| A PROTON MAGNETIC RESONANCE SPECTRAL STUDY OF HEPARIN. L-IDURONIC ACID RESIDUES IN COMMERCIAL HEPARINS, |
22 JAN 1968 |
|
| Authors:
A. S. Perlin; M. Mazurek; L. B. Jaques; L. W. Kavanagh; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
|
| The p.m.r. spectra of commercial heparin samples from a variety of sources indicate that there are at least two types of heparin. These types are differentiated by small, but distinct, variations in the relative intensities of their proton signals and, most noticeably, by the presence of acetyl groups in samples of one of the types. The spectral data also indicate that approximately equimolar proportions of three kinds of constituent sugar ... |
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| UTILIZATION OF ORGANIC NITROGEN COMPOUNDS BY YEASTS OF THE GENUS SACCHAROMYCES, |
1968 |
|
| Authors:
T. A. LaRue; J. F. T. Spencer; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Yeasts belonging to 27 species of Saccharomyces were tested for their ability to use D-amino acids, L-lysine and various amines and amides as nitrogen source. Most yeasts capable of growing on L-lysine or amines could utilize D-amino acids. Saccharomyces (sensu strictu) have a very limited ability to grow on the organic nitrogen compounds tested. However, there is no obvious relationship between the utilization of these compounds and the proposed divisions ... |
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| THE PURIFICATION OF CELLULASE AND EXO-LAMINARANASE AND THEIR ROLE IN THE FORMATION OF PYTHIUM SP. 'PROTOPLASTS', |
01 DEC 1967 |
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| Authors:
J. H. Sietsma; D. E. Eveleigh; R. H. Haskins; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| A cellulase and a laminaranase have been purified from a Streptomyces lytic enzyme complex. Lipase and protease were separated from glucanases. A combination of the purified glucanases produced 'protoplasts' from Pythium sp. PRL 2142. Lipase was shown to act as an adjuvant in this process. (Author) |
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| THE INVOLVEMENT OF CELLULASE AND LAMINARANASE IN THE FORMATION OF PYTHIUM PROTOPLASTS, |
27 NOV 1967 |
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| Authors:
D. E. Eveleigh; J. H. Sietsma; R. H. Haskins; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Five inducible streptomycete lytic enzyme complexes were equally capable of producing protoplasts from a Pythium sp. strain PRL 2142, when compared on a quantitative basis. Both cellulase and laminaranase from various microbial sources were required to produce the protoplasts under standard conditions. Protease was not required; lipase shortened the survival time of protoplasts. It was concluded from an analysis of the products of enzymic hydrolyses that Pythium sp. PRL 2142 ... |
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| TRIDENTATE COMPLEXES OF PERIODATE AND SOME FURANOSE DERIVATIVES, |
04 MAR 1965 |
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| Authors:
A. S. Perlin; E. VON Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| 1,2-O-Isopropylidene-alpha-D-glucofuranose (I) is highly resistant to glycol scission by periodate in alkaline solution. This atypical effect is caused by preferential formation of a stable periodate complex. Proton magnetic resonance spectroscopy shows that the 3-, 5-, and 6-protons of I (and the 3- and 5-protons of I-6,6'-d2) become strongly deshielded during this process, which, together with accompanying changes in proton-proton coupling, establishes that the complex (II) has a 3,5,6tridentate structure. Variations ... |
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| SYNTHESIS OF BETA-D-MANNOSE 1,2ORTHOACETATES, |
04 FEB 1965 |
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| Authors:
M. Mazurek; A. S. Perlin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Synthesis of 3,4,6-tri-O-acetyl-beta-D-mannopyranose 1,2-orthoacetates (I) from 2,3,4,6-tetra-O-acetyl-alphaD-mannopyranosyl bromide (II) and various alcohols in the presence of pyridine and substituted pyridines is described. 2,6-Lutidine is a particularly effective acid acceptor in these condensations, and the use of chloroform as a diluent is advantageous. The lutidine-buffered system has afforded a good yield of orthoacetate (I) with several alcohols that barely react with II in the presence of silver ion; this difference is ... |
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| ACETONATION OF ALDOSE DIETHYL THIOACETALS, |
02 FEB 1965 |
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| Authors:
P. A. J. Gorin; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Acetonation of hexose diethyl thioacetals using copper sulphate catalyst gave rise to 5,6-O-substituted products. The exception was D-galactose diethyl thioacetal, which underwent some 4,5-O-substitution. D-Threose and 2,3-di-O-methyl-D-galactose diethyl thioacetals also underwent predominant alpha-terminal substitution although some beta-terminal substitution took place, particularly with the latter thioacetal. Acetone-sulphuric acid caused preferential alpha-T acetonation of D-threose and 2,3-di-O-methyl-D-galactose diethyl thioacetals. The product from D-threose diethyl thioacetal contained 90% of the 2,3-O-isopropylidene and 10% ... |
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| AMINO ACID RECOVERIES IN THE ANALYSES OF SOME FEED SAMPLES, |
30 NOV 1964 |
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| Authors:
A. J. Finlayson; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Amino acid analyses were made on a sample of rapeseed following oil extraction and on a sample of ground wheat. The analyses show there are hydrolytic losses of threonine, serine, cystine, and methionine which increase with the length of time of hydrolysis of the feed. Recoveries of certain amino acids from feeds depend on the conditions of hydrolysis and hence, for purposes of comparisons of amino acid analyses, hydrolysis conditions ... |
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| MINOR COMPONENTS OF THE OIL OF ARAUCARIA, |
02 OCT 1964 |
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| Authors:
E. VON Rudloff; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| It was shown previously (Chem. and Ind., 1962, 743) that the major constituent (component V, 50.5%) of the oil of araucaria (wood oil of the New Caledonian conifer Neocallitropsis araucarioides (Compt.) Florin syn. Callitropsis araucarioides Compt.) is a mixture of alphaand beta-eudesmol and that component II (8%) is guaiol. Bates and Hendrickson (Chem. and Ind., 1962, 1759) have reported that component III (19.5%), from which a small amount of alpha-cadinol ... |
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| THE OXYGENATED FATTY ACIDS FROM THE OIL OF THE SPORES OF LYCOPODIUM SPECIES, |
01 OCT 1964 |
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| Authors:
A. P. Tulloch; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| The oil from the spores of commercial Lycopodium, mainly L. complanatum, contains cis-9,10-epoxyoctadecanoic acid (2%), (+)-8-hydroxyhexadecanoic acid (7%), and (=)threo-9,10-dihydroxyoctadecanoic acid (4%). Smaller amounts of 8-oxohexadecanoic acid and an epoxyhexadecanoic acid also seem to be present. (Author) |
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| BIOSYNTHESIS OF MUSTARD OIL GLUCOSIDES: V. FORMATION OF GLUCONASTURTIIN FROM L-GAMMAPHENYLBUTYRINE-C(14)-N(15) IN WATERCRESS, |
12 AUG 1964 |
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| Authors:
E. W. UNDERHILL; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| C(14)-Labelled compounds were fed to watercress (Nasturtium officinale R. Br.) and their efficiency as precursors of the aglycone portion of gluconasturtiin compared. Phenylalanine-2- and -3-C(14) and sodium acetate-2-C(14) were efficient precursors of the aglycone but neither compound was as efficient a precursor as gamma-phenylbutyrine (2-amino-4-phenylbutyric acid). Approximately 40% of the C(14) from gamma-phenylbutyrine-2- or -3-C(14) was incorporated into the aglycone side chain. Results of studies with doubly labelled L-gamma-phenylbutyrine-C(14)-N(15) show ... |
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| BIOSYNTHESIS OF MUSTARD OIL GLUCOSIDES. VI. BIOSYNTHESIS OF GLUCOBARBARIN IN RESEDA LUTEOLA L, |
12 AUG 1964 |
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| Authors:
E. W. UNDERHILL; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| A number of C(14)-labelled compounds were fed to Reseda luteola L.; after a 24-hour period of metabolism, the thioglucoside aglycone (5-phenyl-2-oxazolidinethione) was isolated and its specific activity determined. In some instances the aglycone was degraded to determine the distribution of C(14). DL-gammaPhenylbutyrine (2-amino-4-phenylbutyric acid) was the most efficient precursor of the aglycone, followed by phenylalanine and acetate; the carboxyl carbon of these compounds was not incorporated into the thioglucoside aglycone. ... |
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| BIOSYNTHESIS OF THE COUMARINS. VI. FURTHER STUDIES ON HERNIARIN FORMATION IN LAVENDER, |
03 AUG 1964 |
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| Authors:
Stewart A. Brown; NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB
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| Analyses of lavender plants, with careful precautions to avoid enzymic hydrolysis, have shown that herniarin exists in the plant at least 99% in a bound form, presumably cis-2-glucosyloxy-4-methoxycinnamic acid (cis-GMC). Traces of a glycoside of 2-hydroxy-4methoxycinnamic acid (HMC), which appears identical with trans-GMC, have also been identified. Several pieces of evidence, principally the failure to demonstrate conversion of p-coumaric acid to p-methoxycinnamic acid by a trapping technique, now indicate that ... |
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