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KEESLER TECHNICAL TRAINING CENTER KEESLER AFB MS TELECOMMUNICATIONS SYSTEMS STAFF OFFICER COURSE


Click on the titles below to find US government-authored or -collected reports written by KEESLER TECHNICAL TRAINING CENTER KEESLER AFB MS TELECOMMUNICATIONS SYSTEMS STAFF OFFICER COURSE

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Molecular Cloning and Sequencing of the 26S Region of Western Equine Encephalitis Virus Strain 71V-1658 JAN 1997 66 pages
Authors:  Fay L. Schmaltz; Les P. Nagata; George Rayner; KEESLER TECHNICAL TRAINING CENTER KEESLER AFB MS TELECOMMUNICATIONS SYSTEMS STAFF OFFICER COURSE
The full text of this report is available for sale.A 3100 bp cDNA clone of the 26S region of western equine encephalitis (WEE) virus strain 71V-1658 was identified by dot blot hybridization from a cDNA library. The missing 5' end was PCR-amplified and engineered into pcDW-12 to obtained a full length clone of the 26S region. The resulting construct (XH-7) was restriction mapped and completely sequenced on both strands. Only eleven of the sixty-three nucleotide differences resulted in amino ...


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