| Regulation of Progesterone Receptors in Normal and Breast Cancer Cells through Differential Expression of microRNAs |
SEP 2006 |
9 pages |
| Authors:
Eileen McGowan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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 | MicroRNAs are small non-coding RNAs that have an important function in post-translational gene regulation and have been shown to modulate the expression of developmentally important transcription factors. Dysregulation of specific microRNAs has been associated with several cancer types. The study of microRNAs and their potential role in breast cancer is an under- explored area of breast cancer research. This project was designed to explore the possible link between microRNAs and ... |
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| Discovery of Novel Mammary Developmental and Cancer Genes Using ENU Mutagenesis |
SEP 2005 |
22 pages |
| Authors:
Christopher Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| This project sought to discover new genes involved in mammary gland carcinogenesis using ENU mutagenesis. The initial approach involved screening ENU mutagenisized mice for mammary tumors. Screening of 1100 mice by mammary whole mount histology identified a number of pedigrees with mammary tumors attributable to ENU-induced recessive mutation. The authors failed in all cases to recapitulate the original phenotype in subsequent rounds of breeding, indicating the influence of environmental mutagenic ... |
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| Discovery of Novel Mammary Developmental and Cancer Genes Using ENU mutagenesis |
OCT 2004 |
8 pages |
| Authors:
Christopher J. Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Discovery of novel mammary developmental and cancer genes using ENU mutagenesis This project seeks to discover new genes involved in mammary gland carcinogenesis, by screening for reduced latency to palpable mammary tumors in response to the myc oncogene, that is produced by the loss of a second gene knocked out by random ENU mutagenesis. This project draws upon the large scale recessive ENU mutagenesis project at the John Curtin School ... |
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| The Role of the GAB2 Docking Protein in Human Breast Cancer |
JUL 2004 |
23 pages |
| Authors:
Tilman Brummer; Roger J. Daly; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Deregulated receptor-tyrosine kinase (RTK) signalling is a hallmark of many breast cancers and therefore RTKs and their associated signal transducers have emerged as targets for novel therapeutic strategies as well as markers for patient prognosis. Docking proteins of the Gab/Dos family amplify RTK signals and couple these to the Ras/MAPK and Pl-3K pathways, two major signaling pathways with well established roles in tumorigenesis. Expression of the Gab2 isoform is often ... |
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| Investigation of the Link Between Prolactin, Mammary Gland Development and Carcinogenesis by Transcript Profiling |
OCT 2002 |
13 pages |
| Authors:
Jessica Harris; Christopher J. Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Mammary lobuloalveolar development during pregnancy is controlled by the endocrine action of prolactin, estrogen and progesterone, but currently little is known of the transcriptional events that occur in response to these hormones. This study examines the transcriptional response of the mammary gland to the direct action of prolactin. Mammary tissue recombination experiments demonstrated that lobuloalveolar development is dependent upon the presence of prolactin receptors in mammary epithelium but not the ... |
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| Discovery of Novel Mammary Developmental and Cancer Genes Using ENU mutagenesis |
OCT 2002 |
29 pages |
| Authors:
Christopher J. Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Discovery of novel mammary developmental and cancer genes using ENU mutagenesis. The rate of death from breast cancer has only begun to fall in recent years To dramatically reduce breast cancer death rates we need new therapeutic targets, currently a major challenge facing cancer researchers This requires an understanding of the undiscovered pathways that operate to drive breast cancer cell proliferation, cell survival and cell differentiation, pathways which are also ... |
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| Cyclin D1 and Cyclin E as Markers of Therapeutic Responsiveness in Breast Cancer |
MAY 2002 |
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| Authors:
Rina Hui; Robert L. Sutherland; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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 | Cyclin Dl and cyclin E are overexpressed in approximately 43% and 30% of breast cancer and overexpression of these two oncogenes is associated with poor prognosis. To define the role of cyclin Dl and cyclin E expression as markers of therapeutic responsiveness, cell lines overexpressing cyclin Dl or cyclin E were produced. Constitutive overexpression of cyclin Dl in the range of 5 fold and cyclin E in the range of ... |
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| Discovery of a Novel Mammary Developmental and Cancer Genes Using Recessive ENU Mutagenesis |
SEP 2001 |
13 pages |
| Authors:
Christopher J. Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| A screen of the first round of large scale mutagenesis has been completed. Six pedigrees with members harboring mammary tumors were identified. These pedigrees were recreated from genetic backups and are now being screened using mammary biopsy. Affected females will be used to establish stable breeding lines. |
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| Investigation of the Role of the Mitogenic Neuropeptide Galanin in Mammary Gland Development and Carcinogenesis |
AUG 2001 |
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| Authors:
Christopher Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| The neuropeptide galanin is involved in the control of a number of physiological functions including neuronal development and survival, neuroendocrine responses and mitogenesis. We report that null mutation of the galanin gene produced mice with reduced mammary ductal side branching during puberty and reduced lobuloalveolar development with lactation failure following pregnancy. Galanin and galanin receptor 2 (GALR2) are expressed in the mammary gland and are differentially regulated during mammopoiesis. Mammary ... |
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| A Potential Tumor Suppressor Protein: Expression and Function in Human Breast Cancer Cells |
JUL 2001 |
10 pages |
| Authors:
Michelle Callaghan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| In a search for genes with a role in hormonal control of cell proliferation, we cloned a novel progestin-induced gene, EDD (previously DD5) that appears to be the human homologue of the hyd tumor suppressor gene of Drosophila melanogaster. By amino acid homology with other known proteins it is likely that the EDD protein is an E3 ubiquitin-protein ligase, enzymes which target one or more key proteins for destruction by ... |
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| Role of c-Myc in Estrogen Stimulated Cell Proliferation in Normal and Malignant Breast Epithelial Cells |
JUL 2001 |
31 pages |
| Authors:
Eileen Rogen; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Estrogenic steroids have diverse physiological functions and are critically involved in the pathogenesis of breast cancer. The transcriptional regulator c-Myc has been implicated in estrogen-induced mitogenesis and we are therefore investigating the contribution of c-Myc to various downstream molecular and cellular events after estrogen stimulation. We have now developed a series of cell lines expressing c-Myc and mutants lacking one or both of the conserved 'Myc boxes' required for transcriptional ... |
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| Role of Cyclin D1 and p27 in Steroidal Control of Cell Cycle Progression in the Mammary Gland in Vivo |
JUL 2001 |
10 pages |
| Authors:
Elizabeth A. Musgrove; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Estrogen and progesterone are critically involved in both the development of the mammary gland and the pathogenesis of breast cancer, functions thought to rely on their regulation of cell proliferation. This project aims to complement studies of cell cycle control in breast cancer cells in tissue culture by addressing cell cycle control mechanisms in the normal mammary gland. Of particular interest are the roles of cyclin D1 and p27, which ... |
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| Cyclin D and Cyclin E as Markers of Therapeutic Responsiveness in Breast Cancer |
MAY 2001 |
10 pages |
| Authors:
Rina Hui; Robert L. Sutherland; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| There is now accumulating evidence to suggest that aberrant expression of cyclin D1 and cyclin E occurs in human breast cancer and overexpression of these two oncogenes is associated with poor prognosis in prirnary breast cancers. The role of cyclin D1 and cyclin E expression as markers of therapeutic responsiveness to cancer treatment was investigated using cell lines constitutively overexpressing cyclin D1 or cyclin E. In the first mstance, we ... |
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| Investigation of the Role of the Mitogenic Neuropeptide Galanin in Mammary Gland Development and Carcinogenesis |
AUG 2000 |
27 pages |
| Authors:
Christopher Ormandy; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
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| Mammary gland development in the galanin knockout mouse has been the focus of activity during the previous year. The galanin knockout was found to show failure of mammary development during the first pregnancy which resulted in a 100% failure of lactation. Subsequent pregnancies resulted in normal development and successful lactation. Mammary gland recombination/ transplantation showed that this effect was not mammary epithelial cell autonomous, and did not require stromal galanin, ... |
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| Role of c-Myc in Estrogen Stimulated Cell Proliferation in Normal and Malignant Breast Epithelial Cells |
JUL 2000 |
9 pages |
| Authors:
Eileen Rogan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| Estrogenic steroids have diverse physiological functions and are critically involved in the pathogenesis of breast cancer. The transcriptional regulator c-Myc has been implicated in estrogen-induced mitogenesis and we are therefore investigating the contribution of c-Myc to various downstream molecular and cellular events after estrogen stimulation. Transcriptional activation of c-myc is an early response to estrogen treatment and we have previously shown that induction of c-Myc quantitatively reproduces the effects of ... |
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| A Potential Tumor Suppressor Protein: Expression and Function in Human Breast Cancer Cells |
JUL 2000 |
10 pages |
| Authors:
Michelle Callaghan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| In a search for genes with a role in hormonal control of cell proliferation, we cloned a novel progestin-induced gene, EDD (previously DD5) that appears to be the human homologue of the hyd tumor suppressor gene of Drosophila melanogaster. By amino acid homology with other known proteins it is likely that the EDD protein is an E3 ubiquitin-protein ligase, enzymes which target one or more key proteins for destruction by ... |
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| Cyclin Dl and Cyolin E as Markers of Therapeutic Responsiveness in Breast Cancer |
MAY 2000 |
8 pages |
| Authors:
Rina Hui; Robert L. Sutherland; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| There is now accumulating evidence to suggest that aberrant expression of cyclin D 1 and cyclin E occurs in human breast cancer and overexpression of these two oncogenes is associated with poor prognosis in primary breast cancers. To define the role of cyclin D1 and cyclin E expression as markers of therapeutic responsiveness to cancer treatment, cell lines inducibly overexpressing cyclin D1 or cyclin E ... |
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| A Potential Tumor Suppressor Protein: Expression and Function in Human Breast Cancer Cells |
JUL 1999 |
23 pages |
| Authors:
Michelle J. Callaghan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| In a search for genes with a role in hormonal control of cell proliferation, we cloned a novel progestin-induced gene, EDD (previously DD5) that appears to be the human homologue of the hyd tumor suppressor gene of Drosophila melanogaster. By amino acid homology with other known proteins it is likely that the EDD protein is an E3 ubiquitin-protein ligase, enzymes which target one or more ... |
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| Role of c-Myc in Estrogen Stimulated Cell Proliferation in Normal and Malignant Breast Epithelial Cells |
JUL 1999 |
25 pages |
| Authors:
Eileen M. Rogan; GARVAN INST OF MEDICAL RESEARCH SYDNEY (AUSTRALIA)
|
| This project focuses on the role of c-Myc in mediating estrogen actions in breast epithelial cells, specifically to determine the contribution of c-Myc to downstream molecular and cellular events after estrogen stimulation. The rationale for this investigation is, 1) the established intrinsic role of estrogens in mammary gland development and etiology of breast cancer and the proven therapeutic efficacy of antiestrogens in breast cancer, and 2) ... |
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Reports by Corporate Author
