CONNECTICUT UNIV HEALTH CENTER FARMINGTON DEPT OF BIOCHEMISTRY
Click on the titles below to find US government-authored or -collected reports written by CONNECTICUT UNIV HEALTH CENTER FARMINGTON DEPT OF BIOCHEMISTRY
Highlights of work on spores of Bacillus subtilis in the past performance period have included demonstration that: (1) the zymogen of the spore protease GPR autoactivates, and that autoactivation is stimulated by low pH, dipicolinic acid and dehydration; (2) most of the propeptide of GPR is not needed for zymogen stability or autoprocessing; (3) the decrease in forespore pH in B. subtilis sporulation is responsible for 3-phosphoglycerate accumulation; (4) alpha/ ...
Determination by amino acid analyses of the percentage of diaminopimelic acid in the spore cortex of Bacillus megaterium and Bacillus subtilis which is involved in interpeptide cross-links gave values of 31 to 37%. This finding supports the idea that the cortex volume could undergo significant changes in response to changes in pH or ionic strength and could thus play an active role in reducing the water content of the spore ...
Highlights of research findings in the past three years are: (1) A novel enzyme which uses NADH to reduce disulfides (such as oxidized Coenzyme A) containing 4-phosphopantethine residues has been purified to homogeneity from Bacillus megaterium spores and characterized; it is a new member of the class of pyridine nucleotide dependent disulfide reductases. The enzyme is present in a number of gram positive spore formers, but not in gram negative ...
Metabolism of several important small molecules has been examined during sporulation and spore germination in Bacillus megaterium. These studies have indicated (1) the major known low molecular weight thiol/disulfide in Bacillus species is Coenzyme A (CoA); (2) CoA in growing or sporulating cells is in either an acyl form or as the free thiol, but that in dormant spores about 75% is in a disulfide form with 50% in disulfide ...
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