Previously, the complete genome of western equine encephalitis virus had been cloned and sequenced. This paper describes mammalian expression vectors pCXH-3 and pVHX-6, in which expression of the structural genes of western equine encephalitis virus have been placed under the control of the mammalian CMV promoter. When pCXH-3 or pVHX-6 is expressed using a cell-free transcription/ translation system, in vitro, authentic structural proteins of western equine encephalitis virus are produced ...
A sandwich immunoassay for Newcastle Disease Virus (NDV) was developed using streptavidin-biotin mediated filtration capture and silicon- based semiconductor detection. The assays were carried out in one step whereby the immunoreagents and the antigen (NDV) were mixed together and incubated. The pH sensing capability of the detector, a light addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. Lower limits of detection (LOD) of the ...
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