Usanee Vongsthongsri - Storming Media

Detection of Heat-Stable Enterotoxigenic Escherichia coli by Hybridization with an RNA Transcript Probe

AUG 87

3 pages

Authors: Oralak Chityothin; Orntipa Sethabutr; Peter Echeverria; David N. Taylor; Usanee Vongsthongsri; ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES APO SAN FRANCISCO 96346

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Enterotoxigenic Escherichia coli (ETEC) is usually identified by testing isolates for enterotoxin production in bioassays or serological tests. Alternatively, ETEC can be identified by detecting the genes encoding these enterotoxins by DNA hybridization, a method which has been particularly useful in detecting ETEC in large numbers of specimens. Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. ...