The research supported by this grant is intended to investigate the feasibility of an electrocatalytic guanine oxidation reaction as an mRNA quantification method. This system is to be tested first by quantifying the mRNA of Rak nuclear tyrosine kinase in both cell culture and breast tissue samples. To accomplish this goal a competitive RT-PCR assay was designed to measure the absolute quantity of Rak mRNA in each sample. This absolute ...
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