Delvena R. Thomas-Dunmey - Storming Media

Use of the Comet Assay to Detect DNA Damage in Sulfur Mustard-Exposed Human Peripheral Blood Lymphocytes

JUL 1999

27 pages

Authors: Janet Moser; Claire F. Levine; Delvena R. Thomas-Dunmey; Anthony J. Callan; William J. Smith; ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

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Sulfur mustard (HD) is a vesicating agent that alkylates cellular DNA and produces DNA strand breaks. The comet assay (single-cell gel electrophoresis) was used to detect HD-induced DNA single strand breaks in human peripheral blood lymphocytes (PBL). Specific steps taken to perform the comet assay in our laboratory are outlined. Briefly, PBL were mixed with agarose, mounted on a microscope slide, and lysed. The cellular DNA was treated with an ...