We compared the effects of prostaglandin D2 (PGD2), prostaglandin F2 alpha (PGF2 alpha) and various ketones on O2- release by human neutrophils, which had been stimulated by N-formyl methionyl leucyl phenylalanine (FMLP). Our data suggests that the ring carbonyl of PGD2 is essential to its inhibitory effect on O2- release, but the carbonyl group as a ketone, alone, is not sufficient. Using the fluorescent Ca(2+) probe, Fura-2AM, we found that ...
Several hormones act at the cellular level to increase diacylglycerol via increased catabolism of phosphatidylinositol by phospholipase C (PLC). Diacyglycerol stimulates protein kinase C, leading to protein phosphorylation and hormone action. Since PLC activity has not been well studied in man, we have established an assay for PLC in human neutrophils. In this assay sonicates of neutrophils were incubated with L-3- phosphatidyl (U14C)-inositol and the incubation mixture extracted with chloroform:methanol. ...
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