| EGF-Receptor Signaling in Endocytosis Deficient Cells |
JUL 2001 |
24 pages |
| Authors:
Brian Ceresa; Sandra L. Schmid; OKLAHOMA UNIV HEALTH SCIENCES CENTER OKLAHOMA CITY
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 | Award DAMDl7-99-l-9367 seeks to understand the role of membrane trafficking in Epidermal Growth Factor Receptor (EGFR) signal transduction. We have been using a tissue culture model system (HeLa cells) to isolate the activated EGFR at distinct stages in the endocytic pathway. Our research has focused on rab5, a small molecular weight GTPase, implicated in the biogenesis of the early endosome. Mutations to modulate the guanine nucleotide binding properties of this ... |
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| Characterization of Ligand-Induced Endocytosis of EGF-Receptors |
DEC 1997 |
24 pages |
| Authors:
Sandra L. Schmid; SCRIPPS RESEARCH INST LA JOLLA CA
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| Under the auspices of this training Fellowship (May 9, 1994-November 9, 1997) I have undertaken studies on the molecular mechanisms that govern ligand-induced endocytosis of epidermal growth factor receptors (EGFR) and the role of regulated endocytosis of EGFR in controlling EGFR signaling. To this end, I developed a novel cell-free assay which reconstitutes the ligand-induced and EGFR tyrosine kinase-dependent recruitment of EGFR into cathrine coated pits. I also showed that ... |
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| Characterization of Ligand-Induced Endocytosis of EGF - Receptors |
JUN 96 |
24 pages |
| Authors:
Sandra L. Schmid; Christophe Lamaze; SCRIPPS RESEARCH INST LA JOLLA CA
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| We have made progress this year in engineering and using a cell line that is conditionally defective in receptor-mediated endocytosis to directly assess the role of endocytosis in controlling cellular responses to activated EGF-R. We find, not surprisingly, that cells defective in endocytosis show a stronger proliferative response to FGF than wt cells. Surprisingly, we find that some EGF-R signaling pathways, specifically the MAP kinase activation and EGF-R phosphorylation itself ... |
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| Characterization of Ligand-Induced Endocytosis of EGF Receptors |
17 MAY 95 |
10 pages |
| Authors:
Sandra L. Schmid; Christophe Lamaze; SCRIPPS RESEARCH INST LA JOLLA CA
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| Down-regulation of activated EGF-receptors (EGF-R) requires their tyrosine kinase (Y-kinase) activity. However, controversy existed as to whether ligand-induced activation of the EGF-R Y-kinase was required for internalization or for lysosomal targeting. We have addressed this issue using a cell-free assay which selectively measures the recruitment of EGF-R into coated pits. While EGF bound to wild-type receptors is efficiently sequestered in coated pits, sequestration of kinase- deficient receptors occurs inefficiently and ... |
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