| A Quantitative Electrochemiluminescence Assay for Clostridium perfringens alpha toxin |
10 AUG 2006 |
8 pages |
| Authors:
Gerald A. Merrill; Victor R. Rivera; Dwayne D. Neal; Charles Young; Mark A. Poli; BROOKE ARMY MEDICAL CENTER FORT SAM HOUSTON TX
|
 | Described is a rapid direct sandwich format electrochemiluminescence assay for identifying and assaying Clostridium perfringens alpha toxin. Biotinylated antibodies to C. perfringens alpha toxin bound to streptavidin paramagnetic beads specifically immunoadsorbed soluble sample alpha toxin which subsequently selectively immunoadsorbed ruthenium (Ru)-labeled detection antibodies. The ruthenium chelate of detection antibodies chemically reacted in the presence of tripropylamine and upon electronic stimulation emitted photons (electrochemiluminescence) that were detected by the photodiode of ... |
|
| An Electrochemiluminescence-Based Competitive Displacement Immunossay for the Type-2 Brevetoxius in Oyster Extracts |
24 JUL 2006 |
7 pages |
| Authors:
Mark A. Poli; Victor R. Rivera; Dwayne D. Neal; Daniel G. Baden; Shawn A. Messer; Steven M. Plakas; Robert W. Dickey; Kathleen E. Said; Leanne Flewelling; David Green; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80% methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100% acetone extracts, aqueous homogenates, and the clinical matrixes urine and serum can also be analyzed without significant matrix interferences. The assay offers the advantages of speed (2 h analysis time); ... |
|
| Rapid Detection of Clostridium botulinum Toxins A, B, E, and F in Clinical Samples, Selected Food Matrices, and Buffer Using Paramagnetic Bead-Based Electrochemiluminescence Detection |
20 MAR 2006 |
11 pages |
| Authors:
Victor R. Rivera; Frank J. Gamez; William K. Keener; Jill A. White; Mark A. Poli; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| Sensitive and specific electrochemiluminescence (ECL) assays were used to detect Clostridium botulinum neurotoxins serotypes A, B, E, and F in undiluted human serum, undiluted human urine, assay buffer, and selected food matrices (whole milk, apple juice, ground beef, pastry, and raw eggs). These novel assays used paramagnetic bead-based electrochemiluminescent technology in which biotinylated serotype-specific antibodies were bound to streptavidin-coated paramagnetic beads. The beads acted as the solid support and captured ... |
|
| An Activity-Dependent Assay for Ricin and Related RNA N-Glycosidases Based on Electrochemiluminescence |
2006 |
9 pages |
| Authors:
William K. Keener; Victor R. Rivera; Charles C. Young; Mark A. Poli; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
|
| Synthetic biotinylated RNA substrates were cleaved by the combined actions of ricin holotoxin and a chemical agent, N,N'-dimethylethylenediamine. The annealing of the product with a ruthenylated oligodeoxynucleotide resulted in the capture of ruthenium chelate onto magnetic beads, enabling the electrochemiluminescence (ECL)-based detection of RNA N-glycosidase activities of toxins. ECL immunoassays and the activity assay exhibited similar limits of detection just below signals with 0.1ng/ml of ricin; the ECL response was ... |
|
Reports by Author
