| Shewanella Oneidensis MR-1 Msh Pilin Proteins are Involved in Extracellular Electron Transfer in Microbial Fuel Cells |
Jan 2011 |
6 pages |
| Authors:
Lisa A Fitzgerald; Emily R Peterson; Richard I Ray; Brenda J Little; Candace J Cooper; Erinn C Howard; Bradley R Ringeisen; Justin C Biffinger; NAVAL RESEARCH LAB WASHINGTON DC CHEMISTRY DIV
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 | Shewanella is a microbial genus that can oxidize lactate for the reduction of insoluble electron acceptors. This reduction is possible by either direct (cell surface interaction, nanowires) or indirect (soluble redox mediators) mechanisms. However, the actual molecular identification of a nanowire has not been determined. Through mutational studies, Shewanella oneidensis MR-1 was analyzed for its ability to transfer electrons to an electrode after deletion of the structural pilin genes ( ... |
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| Microbial Gas Production Used to Achieve Autonomous Buoyancy Control |
01 Sep 2010 |
12 pages |
| Authors:
Peter K Wu; Lisa A Fitzgerald; Justin C Biffinger; Barry J Spargo; Bradley R Ringeisen; Brian H Houston; Joseph Bucaro; NAVAL RESEARCH LAB WASHINGTON DC CHEMICAL DYNAMICS AND DIAGNOSTICS BRANCH
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| We have constructed and successfully laboratory tested a device that can periodically change from a submerged to a buoyant state using gas generated by microbes alone. The duration of the buoyant state and the switching frequency from the submerged to buoyant state can be controlled. If the type of microbes used is native to the deployment location, gas generation will not create an identifiable acoustic signature disclose the presence and ... |
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| The Role of Shewanella oneidensis MR-1 Outer Surface Structures in Extracellular Electron Transfer |
Jan 2010 |
10 pages |
| Authors:
Rachida A Bouhenni; Gary J Vora; Justin C Biffinger; Sheetal Shirodkar; Ken Brockman; Ricky Ray; Peter Wu; Brandy J Johnson; Eulandria M Biddle; Matthew J Marshall; Lisa A Fitzgerald; Jim K Fredrickson; Alexander S Beliaev; Bradley R Ringeisen; Daad A Saffarini; NAVAL RESEARCH LAB WASHINGTON DC CHEMISTRY DIV
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| The ability of the metal reducer Shewanella oneidensis MR-1 to generate electricity in microbial fuel cells (MFCs) depends on the activity of a predicted type IV prepilin peptidase; PilD. Analysis of an S. oneidensis MR-1 pilD mutant indicated that it was deficient in pili production (Msh and type IV) and type II secretion (T2S). The requirement for T2S in metal reduction has been previously identified, but the role of pili ... |
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| Probing Electron Transfer Mechanisms in Shewanella oneidensis MR-1 using a Nanoelectrode Platform and Single-Cell Imaging |
Jan 2010 |
6 pages |
| Authors:
Xiaocheng Jiang; Jinsong Hu; Lisa A Fitzgerald; Justin C Biffinger; Ping Xie; Bradley R Ringeisen; Charles M Lieber; NAVAL RESEARCH LAB WASHINGTON DC CHEMISTRY DIV
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| Microbial fuel cells (MFCs) represent a promising approach for sustainable energy production as they generate electricity directly from metabolism of organic substrates without the need for catalysts. However, the mechanisms of electron transfer between microbes and electrodes, which could ultimately limit power extraction, remain controversial. Here we demonstrate optically transparent nanoelectrodes as a platform to investigate extracellular electron transfer in Shewanella oneidensis MR-1, where an array of nanoholes precludes or ... |
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| The Influence of Acidity on Microbial Fuel Cells Containing Shewanella Oneidensis (PREPRINT) |
01-Sep-2008 |
9 pages |
| Authors:
Justin C Biffinger; Jeremy Pietron; Orianna Bretschger; Lloyd J Nadeau; Glenn R Johnson; Cynthia C Williams; Kenneth H Nealson; Bradley R Ringeisen; NAVAL RESEARCH LAB WASHINGTON DC CHEMISTRY DIV
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| Microbial fuel cells (MFCs) traditionally operate at pH values between 6 and 8. However, the effect of pH on the growth and electron transfer abilities of Shewanella oneidensis MR-1 (wild-type) and DSP10 (spontaneous mutant), bacteria commonly used in MFCs, has not been characterized. Miniature MFCs using bare graphite felt electrodes and nanoporous polycarbonate membranes with MR-1 or DSP10 cultures generated > 8 W/cu m and ~ 400 microA between pH ... |
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