Dipicolinic acid (DPA) and the Ca2+ complex of DPA (CaDPA) are major chemical components of bacterial spores. With fluorescence being considered for the detection and identification of spores, it is important to understand the optical properties of the major components of the spores. In this paper we report in some detail on the room temperature fluorescence excitation and emission spectra of DPA and its calcium ion complex and comparison of ...
Early discussions held prior to the Army's recent decision to re- examine the use of UV fluorescence for remote detection of biological agents are reviewed. Samples of sand from five different locations in northeastern Saudi Arabia were analyzed for UV excited fluorescence emission as well as microbial content. The fluorescence emission is compared to that typical for growing bacteria and for bacterial spores. On the basis of this comparison, it ...
We have observed the light scattering transients arising from changes in the curvature of purple membrane fragments upon photoexcitation at p11 8.05 and 4.1 with and without treatment of Triton X-100. The low ionic strength room temperature suspensions are excited with 532 nm light pulses from a Nd:YAG laser (20 ns). The scattering of 320 nm light is monitored from 3 us to 1 S at scattering angles from 150 ...
Laser light is the brightest monochromatic (single color) light existing today. Besides being a standard tool of the research lab, the laser is currently used in communications, surveying, manufacturing, diagnostic medicine and surgery. Supermarket bar code scanners and the compact disk player have even moved lasers into everyday life. The addition of lasers and the development of new lasers to the surgical armamentarium in otolaryngology--head and neck surgery offers new ...
In order to investigate and understand the dynamics of protein fluctuations, specifically the interaction between dynamical structure and function, we have started a program to use resonantly enhanced light scattering to probe the fluctuation spectrum. Here we report on a new technique, the use of a quartz microbalance to confirm the existence of motion within myoglobin at 5 MHz. We report on modelling the temperature rise for an absorbing sample ...
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