Monica O'Guinn - Storming Media

Dual-Probe Real-Time PCR Assay for Detection of Variola or Other Orthopoxviruses with Dried Reagents

10 Sep 2008

7 pages

Authors: Mohamed Aitichou; Sharron Saleh; Park Kyusung; John Huggins; Monica O'Guinn; Peter Jahrling; Sofi Ibrahim; ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD VIROLOGY DIV

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A real-time, multiplexed PCR assay based on dried PCR reagents was developed. Only variola virus could be specifically detected by a FAM (6-carboxyfluorescein)-labeled probe while camelpox, cowpox, monkeypox and vaccinia viruses could be detected by a TET (6-carboxytetramethylrhodamine)-labeled probe in a single PCR reaction. Approximately 25 copies of cloned variola virus DNA and 50 copies of genomic orthopoxviruses DNA could be detected with high reproducibility. The assay exhibited a dynamic ...

Habitats and Distribution of Anopheles Sinensis and Associated Anopheles Hyrcanus Group in Japan

Jan 2005

7 pages

Authors: Leopoldo M Rueda; Masashiro Iwakami; Monica O'Guinn; Motoyoshi Mogi; Brian F Prendergast; Ichiro Miyagi; Takado Toma; James E Pecor; RichArd C Wilkerson; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF ENTOMOLOGY

Mosquito collections were carried out in August 2002 and July 2003 in Japan. Anopheles sinensis of the Hyrcanus Group. Myzomyia Series of Anopheles. was the most common species encountered. The distribution and habitats of 5 Anopheles Hyrcanus Group species are included. Eight species belonging to Aedes. Cllle.C. and Uranotal'1lia were found associated with An. sinensis in rice paddies and a variety of other larval habitats.