Sulfur mustard (HD) is a vesicating agent that alkylates cellular DNA and produces DNA strand breaks. The comet assay (single-cell gel electrophoresis) was used to detect HD-induced DNA single strand breaks in human peripheral blood lymphocytes (PBL). Specific steps taken to perform the comet assay in our laboratory are outlined. Briefly, PBL were mixed with agarose, mounted on a microscope slide, and lysed. The cellular DNA was treated with an ...
Reports by Author
