R. J. Mioduszewski - Storming Media

Fluoride Ion Regeneration Oo Cyclosarin (Gf) From Minipig Tissue and Fluids Following Whole Body Gf Miosis Level Vapor Exposure

16 NOV 2004

25 pages

Authors: E. M. Jakubowski; J. M. McGuire; R. A. Evans; S. W. Hulet; D. C. Burnett; R. A. Way; B. I. Gaviola; J. A. Scotto; W. T. Muse; D. B. Miller; R. J. Mioduszewski; S. A. Thomson; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD

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Chemical warfare nerve agent biomarker methods: 1) Cholinesterase activity, 2) Nerve agent hydrolysis products, 3) Fluoride ion regenerated alkyl methylphosphonofluoridate (G agents): a) human plasma, b) red blood cells, c) tissue. New analytical method: 1) ammonia CI, 2) a large volume injector (LVI) with Tenax insert, and 3) stable isotope (2H11-GF) internal standard.

Analysis of Rat Blood Samples for Agent Biomarkers After GB Inhalation Exposure

JAN 2002

7 pages

Authors: E. M. Jakubowski; L. S. Heykamp; R. J. Mioduszewski; J. Manthei; D. C. Burnett; R. Way; B. Gaviola; W. Muse; J. Anthony; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD RESEARCH AND TECHNOLOGY DIR

A method was developed for the analysis of a GB nerve agent biomarker in blood that is very sensitive, selective, and applicable for archived samples. The biomarker resulting from sample acidification and in the presence of fluoride ion was regenerated GB (rGB) which was found in rat blood after inhalation exposures ranging from miosis to lethal levels. The method utilized a C18 solid-phase extraction (SPE) followed by quantification using a ...