We study the process of DNA replication in proliferating human cells. Our efforts are directed to the identification and characterization of proteins that promote DNA replication ("initiators") as well as the DNA sequences recognized by them ("replicators") . We have focused in a group of initiator proteins called Origin Recognition Complex (ORC), Cdc6, and the Mini-Chromosome Maintenance (MCM) proteins. hOrclp, the largest subunit of ORC, appears to be a critical ...
Our goal is to understand the process of DNA replication at the molecular level in human cells. We are specifically trying to identify human replicator sequences and to characterize the pre-replicative complexes at the origins of replication. In this report we summarize a series of experiments using chromatin immunoprecipitation techniques and discuss the technical problems associated with them. Then we evaluate the stability of the human origin recognition complex across ...
The coordination between cellular DNA replication and mitosis is critical to ensure controlled cell proliferation and accurate transmission of the genetic information as cells divide -two aspects of cellular life tipically lost in cancer. In order to unravel the molecular mechanisms of human DNA replication in normal and cancer cells, we have started a search for human DNA sequences that serve as replicators", this is, binding sites for human proteins ...
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