The optimized conditions for the production of levan by Erwinia herbicola were determined to be pH (6.0-7.5), incubation time (72 hours), culture media (K2HPO4, 3.0g; NaC1, 2.0g; Urea, 0.3g; MgSO4.7H2O, 0.4g; FeSO47H2O, 0.10g, 5% sucrose per liter), and polymer purification (sodium dodecyl sulfate treatment, dilution with H2O, dialysis, and methanol precipitation). While continuous culture fer;mentation at pH 7.2 produced the highest yield of polymer, molecular weight determinations of the isolated ...
In order to determine optimum environmental conditions for t he production of elsinan, the following parameters were evaluated: culture conditions, growth medium, incubation period, ph, and sources and amounts of carbon, nitrogen, and phosphate. A medium was devised for the optimum yields of high (>2 million), medium (1-2 million), and low (<1 million) weight average molecular weight biopolymer products. Batch systems, scale-up batch, and continuous fermentations of one liter and ...
This report summarizes one section of this study, the evaluation of environmental conditions and their influence on the biopolymer pullulan molecular weight distribution and yield. Films, fibers, and chemical derivatives made from these different molecular weight products, and the characterization of these materials will provide the bases for another report. In order to determine optimum growth conditions for the control of molecular weight distribution of extracellular pullulan, preliminary studies utilized ...
Permethrin, niclosamide, and EDOLAN U pesticides were extracted from Battle Dress Uniform (BDU) and wool materials and analyzed by gas chromatography (GC) in order to determine the recovery efficiency of these compounds from dyed materials using Soxhlet extraction followed by GC analysis. Permethrin and EDOLAN U extraction and quantification were both simple and accurate when electron capture detection was used because dye interferences were eliminated. Niclosamide analysis by GC was ...
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