| The Pathophysiology of Combined Injury and Trauma: Proceedings of the International Symposium (1st) Held at the Uniformed Services University of the Health Sciences, Bethesda, Maryland on 27-29 April 1983 |
29 APR 1983 |
494 pages |
| Authors:
R. I. Walker; D. F. Gruber; T. J. MacVittie; J. J. Conklin; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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 | This report is the proceedings of the First International Symposium held 27-29 April 1983 in Bethesda, Maryland, sponsored by the Armed Forces Radiobiology Research Institute. The text is comprised of 29 research papers, which fall into three subject areas: Combined Injury and Trauma, Immunology and Inflammatory Responses, and Sepsis. Also included are an Executive Summary, a Panel Discussion summarizing the presentations, Abstracts of Poster Presentations, and a List of Attendees. ... |
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| Pathophysiology of Combined Injury and Trauma: An Overview, |
29 APR 1983 |
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| Authors:
J. J. Conklin; T. J. MacVittie; R. I. Walker; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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 | It has been 15 years since the problem of combined injuries has been reviewed. Since then a great deal has been learned about the pathophysiology of combined injuries. This proceedings will review the mechanisms in combined injuries, sepsis, immunological and inflammatory responses, surgery and wound healing, and therapy. This paper will briefly review experimental data on combined injury, and try to provide an overview of the primary pathologic processes causing ... |
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| Canine Hematopoiesis in a Model of Combined Injury, |
29 APR 1983 |
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| Authors:
T. J. MacVittie; R. L. Monroy; M. Fink; D. F. Gruber; M. L. Patchen; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| The development of a large animal model for CI within the context of a nuclear disaster required that we describe, experimentally, the essential features of the radiobiology of acute effects in the canine. The large-animal model is also appropriate for assessing the immunologic, pharmacologic, and surgical modes of intervention of following CI. The canine model of CI at the AFRRI has stressed three developmental aspects: (a) establishing the radiobiology of ... |
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| Characteristics of the in Vitro Monocyte-Macrophage Colony-Forming Cells Detected within Mouse Thymus and Lymph Nodes, |
1977 |
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| Authors:
T. J. MacVittie; T. L. Weatherly; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| The enigmatic presence of in vitro colony-forming cells (CFC) within the thymus (T) and lymph node (LN) organs prompted us to determine additional characteristics of this cell population in an effort to examine their relationship to other colony-forming cells (CFC, CFU-c) derived from several hematopoietic sites. Their cluster-to-colony ratio, survival in the absence of colony-stimulating activity (CSA), radiosensitivity, drug sensitivity, and fraction in cell cycle were compared to the same ... |
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| Mitogenic and Colony Forming Unit Responses of Spleen Cells from Mice Engrafted with Lewis Lung (3LL) Carcinoma Cells, |
SEP 1976 |
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| Authors:
G. D. Ledney; J. V. Moniot; M. R. Gambrill; T. J. MacVittie; S. G. Levin; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| An integrated study of the host's hematocytopoietic responses to malignancy was undertaken with the aid of an animal model. The Lewis lung (3LL) transplantable carcinoma produces splenic enlargement in mice engrafted sc with a tumor cell inoculum. Thus, the splenic enlargement was believed to be associated with myelocytopoietic and lymphocytopoietic changes that were dependent on (1) initial 3LL cell load, (2) time after engraftment with 3LL cells and (3) engrafted ... |
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| Colony Forming Capacity of Mouse Thymus Cells 'in vitro'. |
MAR 1976 |
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| Authors:
T. J. MacVittie; K. F. McCarthy; R. M. Crawford; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| A small number of cells within the mouse thymus were induced to form colonies of mononuclear cells in an in vitro agar system. The specific factor necessary to induce colony formation was present in an extract of placenta, memebranes and uterine tissue from pregnant mice. The relative incidence of the colony forming cells was 104:7.5:1 for mouse marrow, spleen and thymus, respectively. This research is part of a program for ... |
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| Mitigation of Graft Versus Host Disease in Lethally Irradiated Mice Grafted with Spleen Cells Adherent to Glass Beads. |
MAR 1976 |
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| Authors:
M. R. Gambrill; G. D. Ledney; T. J. MacVittie; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| Murine spleen cells were separated on the basis of adherence to glass beads into distinct subpopulations that differ in their ability to produce acute graft versus host disease (GVHD). Nonadherent CBA spleen cells produce acute GVHD in 6 to 10 days in lethally irradiated F1 mice as do unfractionated spleen cells. Spleen cells which are adherent to glass beads, however, enable 71 percent of the mice to survive without symptomatology ... |
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| The Influence of a Granulocytic Inhibitor (Chalone) on Hematopoiesis in an 'in vivo' Culture System. |
JUN 1975 |
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| Authors:
T. J. MacVittie; K. F. McCarthy; E. G. McCarthy; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| The in vivo diffusion chamber (DC) technique for mouse marrow culture was used to determine the effect of a granulocyte inhibitor (chalone) on the proliferation of the pluripotent stem cell (CFU-S) and the granulocyte progenitor cell (CFU-C). The DC's were each inoculated with a normal mouse marrow suspension and then implanted into host mice exposed four hours previously to 900 rads 60Cogamma-radiation. Granulocyte inhibitor (chalone) containing solution was collected as ... |
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| Regulation of Hemopoietic Stem Cell Turnover and Population Size in Neonatal Mice. |
APR 1975 |
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| Authors:
K. F. McCarthy; T. J. MacVittie; P. J. Kirchgessner; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| Following birth the hematopoietic stem cell population of the liver as measured by the in vivo spleen nodule assay (CFU) declines with a halving time of about 48 hours. The stem cell population of the spleen grows exponentially with a doubling time of about 17 hours. In vitro incubation with high specific activity 3H-TdR and sedimentationn velocity studies indicate that CFU in neonatal liver and spleen are in cell cycle ... |
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| Inhibition of Granulopoiesis in Diffusion Chambers by a Granulocyte Chalone, |
JUL 1974 |
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| Authors:
T. J. MacVittie; K. F. McCarthy; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| Granulocyte chalone obtained from media conditioned by rat granulocytes was tested on normal and vinblastine treated regenerating mouse marrow placed in an in vivo diffusion chamber culture system. Raw granulocyte chalone when injected several times during the early phase of culture significantly altered the cellular growth pattern as well as the parent-progeny relationships of both normal and regenerating marrow established within the chamber milieu. The total number of chamber cells ... |
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| Separation of Spleen Cells in Iso-Osmotic Ficoll Density Gradients. |
MAR 1973 |
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| Authors:
K. F. McCarthy; J. E. Nutter; T. J. MacVittie; ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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| Osmotic pressures of polymer solutions were examined as a function of density by equilibrium dialysis, and it was reaffirmed that the van't Hoff equation cannot be used to calculate the osmotic pressure of polymer solutions at concentrations greater than 5 percent. Therefore, it appears that previous methods utilizing polymer solutions for the generation of density gradients also generated hyperosmotic gradients. However, since cells maintain their integrity best in an isotonic ... |
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