During this demonstration, we evaluated and validated the sue of nucleic acid-based tools for site assessment and bioremediation process monitoring at chlorinated solvent sites undergoing MNA, biostimulation, and/or bioaugmentation treatment. Use of these tools is anticipated to reduce remediation costs by 1. supporting identification of sites amenable to MNA; 2. predicting sites where biostimulation can be successfully implemented; 3. identifying sites where bioaugmentation is required early in the design process, ...
The purpose of this document is to provide guidance to Remedial Project Managers (RPMs) and field practitioners on the application of Molecular Biological Tools (MBTs), specifically nucleic acid based tools, for evaluating monitored natural attenuation (MNA), biostimulation and bioaugmentation at chlorinated solvent sites. This protocol summarizes the current state of the practice of these tools and is intended to provide a technically sound and practical approach to MBT use. This ...