Denaturing high-performance liquid chromatography (DHPLC) has been used extensively to detect genetic variation. We used this method to detect and identify Yersinia pestis KIM5 ciprofloxacin-resistant isolates by analyzing the quinolone resistance-determining region (QRDR) of the gyrase A gene. Sequencing of the Y. pestis KIM5 strain gyrA QRDR from 55 ciprofloxacin-resistant isolates revealed five mutation types. We analyzed the gyrA QRDR by DHPLC to assess its ability to detect point mutations ...
Concerns relating to unidentifiable pathogens that could result from either deliberate or natural mutation processes have prompted studies to find alternative approaches other than the conventional detection methods. Our thesis was that an exposed individual would show gene expression responses unique to the pathogenic agent and prior to onset of the full illness. This study describes our work to establish a library of host responses to pathogenic agents for use ...
Reports by Author
