Incorporation of C14 into the lipids of the obligate halophile, Halobacterium cutirubrum grown in the presence of C14-labelled acetate, malonate, mevalonate or glycerol was investigated. Mevalonate showed the highest incorporation (8% of the amount supplied), acetate and glycerol were each incorporated to the extent of 2%, and only traces of C14 were incorporated with malonate as precursor. Incorporation with the latter precursor is probably due to prior breakdown of the ...
The ribosomes of Halobacterium cutirubrum, a micro-organism that grows in saturated sodium chloride and probably has an internal concentration of potassium chloride near saturation, exist as 70 s particles when isolated in saturated potassium chloride containing 0.1 m-magnesium chloride at pH7. These particles are stable in concentrations of magnesium ions as high as 0.4 m. If the potassium ion concentration is below about 4 m, or the magnesium ion concentration ...
In the electron microscope, isolated cell envelopes of the extremely halophilic bacterium, Halobacterium cutirubrum, have the same hexagonal surface pattern and roughly the same shape as do intact cells. At different pH values and in different ionic environments the envelopes undergo changes in shape and surface structure similar to those of intact cells. Chemical analyses show that envelopes consist mainly of lipoprotein. Both lipid and protein components are negatively charged ...
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