Neurofibromatoses (NF) are disorders caused by mutations in NF1/NF2 genes and characterized by fibromatous tumors on nerves, skin, and bones. A method to rapidly and cheaply re-sequence the NF loci would greatly aid in the molecular classification of the disease by forging links between sequence variations and clinical manifestations. We propose to develop a technique to rapidly re-sequence genomic loci using microarray hybridization and bioinformatics. The NF locus is first ...
We have evaluated two strategies for identifying inhibitors of the DNA repair pathway. We used the yeast reverse two hybrid system to screen for inhibitory peptides of the Rad51 protein in order to disrupt the homologous recombination pathway from a plasmid library coding for 15 amino acid peptides. While we succeeded in generating a library of sufficient complexity, because of inherent technical issues involved we were not successful in identifying ...
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