| Control of the Inflammatory Response |
23 APR 1982 |
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| Authors:
John C. Houck; VIRGINIA MASON RESEARCH CENTER SEATTLE WA
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| Chemical Messengers of Inflammation |
29 APR 1981 |
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| Authors:
John C. Houck; VIRGINIA MASON RESEARCH CENTER SEATTLE WASH
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 | The purpose of this contract is to support pilot studies into the possibility of utilizing various biological molecules obtained as natural products which can control the immunologic aspects of inflammation (i.e. inhibit lymphocyte function) and thereby possibly prolong the survival of organ grafts after transplantation. (Author) |
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| Control of the Inflammatory Process. |
04 APR 1980 |
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| Authors:
John C. Houck; VIRGINIA MASON RESEARCH CENTER SEATTLE WASH
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| We have purified the factor released by lung cells during their cultivation in vitro which permit the mitotic activity of alveolar macrophages. This factor has a molecular weight of 68,000 daltons with an isoelectric point of 4.2 and is in equilibrium with as much as 20% of the total molecular species as dimers, trimers and tetramers. This activity appears to possess the ability to inhibit trypsin and elastase but not ... |
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| Chemical Messengers of Inflammation. |
25 APR 1978 |
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| Authors:
John C. Houck; VIRGINIA MASON RESEARCH CENTER SEATTLE WASH
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| We have been able to isolate, purify and physicochemically characterize three important lymphokines; MIF, the chemotactic stimulant to lymphocytes, (lymphotactin) and Skin Reactive Factor/LNPF. Each of these molecules has been purified to electrophoretic homogeneity at least two different pH in acrylamide gel electrophoresis. The carbohydrate composition of MIF was determined and found to involve only two moieties, sialic acid and neutral sugur which may be O-methylglucopyranoside. Antisera against thymus-derived MIF ... |
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| Protein Chemistry of Necrotic Wounds. |
13 DEC 1976 |
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| Authors:
John C. Houck; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| The author has successfully (1) isolated, purified and chemically characterized MIF (2) isolated, purified and characterized chemically lymphotactin and (3) isolated, purified and partially characterized a lysosomal permeability factor hitherto known as Skin Reactive Factor, and presumed to be lymphokine. The author made the fundamental discovery that the acid leukokinin system is most probably not a cathepsin D activated kinin, but rather is the result of the activity of the ... |
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| Protein Chemistry of Necrotic Wounds, |
10 NOV 1975 |
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| Authors:
John C. Houck; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| Using a newly developed assay procedure for Macrophage migration inhibitory factor (MIF), the authors have shown that thymus from immunologically sophisticated animals contains MIF and other lymphokines. The authors have isolated and completely purified MIF from thymus and have in large part characterized this macromolecule chemically and biologically. A new lymphokine, 'lymphotactin,' has been discovered which is specifically chemotactic for lymphocytes. The authors have also isolated skin reactive factor, which ... |
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| Evaluation of Wound Healing Accelerators-Cartilage Powder, Chitin, and N-Acetyl Glucosamine - in Human Fibroblast Cultures. |
AUG 1973 |
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| Authors:
Michael D. Waters; Robert D. Moore; Joseph J. Amato; Dale H. Heitkamp; John C. Houck; EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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| Crude cartilage powder does not accelerate cellular proliferation in newly established human fetal skin fibroblast cultures at concentrations of 0.3 or 30 microg/ml. Collagen biosynthesis is not increased in confluent cultures of human fetal or adult skin fibroblasts by addition of saline or distilled water extracts of cartilage powder at medium concentrations of 30 or 100 microg/ml. Neither N-acetyl glucosamine nor its polymeric form, chitin, has a consistent stimulatory or ... |
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| Effect of 5,5-Diphenylhydantoin (Dilantin) on Collagen Biosynthesis in Human Skin Fibroblasts. |
JUN 1973 |
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| Authors:
Michael D. Waters; Robert D. Moore; Joseph J. Amato; John C. Houck; EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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| Dilantin sodium in concentrations lower than .0001M does not influence collagen biosynthesis in stationary cultures of diploid human skin fibroblasts. At a concentration of .0001M Dilantin, cellular proliferation as measured by total culture protein is inhibited by approximately 50% as compared to controls. Both cellular proliferation and collagen biosynthesis are almost completely inhibited in the presence of .001M Dilantin. These findings suggest that Dilantin does not increase collagen biosynthesis by ... |
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| Protein Chemistry of Necrotic Wounds, |
1972 |
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| Authors:
John C. Houck; Richard J. DeAngelis; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| Acute inflammation involves the initial and characteristic responses of tissues to trauma. They involve (1) the initial increase in the permeability to microcirculation; (b) the accumulation of firstly, polymorphonuclear (PMN) leukocytes and secondly, mononuclear leukocytes in the wound. It has been shown that collagenolysis, which occurs within the wound space during necrosis, releases peptides of relatively small molecular weight which are chemotactic for PMN, but not lymphocytes or mononuclear cells. ... |
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| Specific Endogenous Mitotic Inhibitors of Human Lymphocyte Transformation, |
1972 |
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| Authors:
John C. Houck; Hiltje Iruasquin; Sanford Leikin; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| On the basis of investigations into the control of epidermal proliferation, and maturation, Bullough and Laurence proposed that epithelial cells make a specific and endogenous mitotic inhibitor ('chalone') which functions to preclude mitosis in the epidermal cells in conjunction with both adrenal medullary and cortical hormones. Subsequent studies by a number of workers have indicated that these specific endogenous mitotic inhibitors (SEMIs) might also be found for melanocytes, granulocytes, kidney ... |
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| Protein Metabolism of Necrotic Wounds. |
22 JUN 1971 |
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| Authors:
John C. Houck; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| The collagen losses in necrotic woulds are associated with an apparent collagenolysis due to the activation of a collagenolytic enzyme. This enzyme is normally complexed with a protein inhibitor which is more sensitive to tryptic digestion than is the collagenolytic enzyme itself. This enzyme releases a wide variety of molecular weight products from purified soluble native collagen ranging from 1,000 to 30,000 daltons in molecular weight. All of these products ... |
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| Zinc Sulfate Failure as Accelerator of Collagen Biosynthesis and Fibroblast Proliferation. |
JUN 1971 |
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| Authors:
Michael D. Waters; Robert D. Moore; Joseph J. Amato; John C. Houck; EDGEWOOD ARSENAL MD
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| The effect of zinc sulfate on collagen biosynthesis and fibroblast proliferation has been studied in a tissue culture model system using human skin fibroblasts. Addition of zinc sulfate to newly established (low density) cultures in concentrations of 0.0001 to 10 to the -8th power M results in concentration-dependent cytotoxicity with inhibition of collagen biosynthesis as well as cellular proliferation. Zinc sulfate at 0.0001 to 0.0000001 M added to confluent (high ... |
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| CONTROL OF HUMAN FIBROBLAST PROLIFERATION. |
20 FEB 1970 |
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| Authors:
John C. Houck; CHILDREN'S HOSPITAL RESEARCH FOUNDATION WASHINGTON D C
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| Diploid human fibroblasts will not divide in the absence of serum macromolecules weighing between 50,000 to 100,000. They are not species specific. These macromolecules have been purified from serum about 10 fold so far. The further purification of these materials and their subsequent characterization is being attempted. The extracellular compartment of skin contains non-dialyzable macromolecules which will inhibit this serum produced stimulation of diploid human fibroblast mitosis. The mitotic stimulation ... |
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Reports by Author
