| The Role of Amplified Wild-Type Neu in the Etiology of Breast Cancer |
JUL 2004 |
22 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
 | Many human breast cancers have amplified wild-type Neu (Her2) protooncogenes. It is still not known from either human or rodent models if amplified wild-type Neu is involved in the etiology of breast cancer, We have begun to develop rat transgenic models. Under the first aim we will construct additional Neu transgenic rat lines. Aim 2 will endocrinologically manipulate female Neu-transgenic rats in a way that models high androgen postmenopausal women. ... |
|
| The Role of Amplified Wild-Type Neu in the Etiology of Breast Cancer |
JUL 2003 |
21 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| Many human breast cancers have amplified wild-type Neu (Her2) protooncogenes. It is still not known from either human or rodent models if amplified wild-type Neu is involved in the etiology of breast cancer. We have begun to develop rat transgenic models. Under the first aim we will construct additional Neu transgenic rat lines. Aim 2 will endocrinologically manipulate female Neu-transgenic rats in a way that models high androgen postmenopausal women. ... |
|
| The Role of Amplified Wild-Type Neu in the Etiology of Breast Cancer |
JUL 2002 |
19 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| Many human breast cancers have amplified wild-type Neu (Her2) protooncogenes. It is still not known from either human or rodent models if amplified wild-type Neu is involved in the etiology of breast cancer. We have begun to develop rat transgenic models. Under the first aim we will construct additional Neu transgenic rat lines. Aim 2 will endocrinologically manipulate female Neu-transgenic rats in a way that models high androgen postmenopausal women. ... |
|
| Mechanisms Underlying the Very High Susceptibility of the Immature Mammary Gland to Carcinogenic Initiation |
JUL 2000 |
44 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| The overall goal of this project was to explore the toxic effects of physical and chemical carcinogens on the immature mammary gland as compared to the effects on the young adult mammary gland using a rat model. We have: I) completed all comparative cytotoxicity studies showing that the immature mammary gland is more sensitive to radiation and NMU but not DMBA, 2) adapted the "Big Blue" mutagenesis assay to the ... |
|
| Investigating the Role of Cooperative Interactions Between the Neu Proto-oncogene and the Other erbB Family Members in Rat Mammary Carcinogenesis |
JUL 2000 |
19 pages |
| Authors:
Michael N. Gould; Philip Watson; WISCONSIN UNIV-MADISON
|
| In order to generate a rat model of neu-induced mammary carcinogenesis, we have created transgenic rats that utilize the mouse mammary tumor virus promoter to overexpress the rat neu proto-oncogene in the mammary gland. A nuclease protection assay was used to quantify the relative overexpression of neu message within the mammary gland and rats of both sexes were observed until at least 14 months of age. In the most characterized ... |
|
| Investigating the Role of Cooperative Interactions Between the Neu Protooncogene and the Other erbB Family Members in Rat Mammary Carcinogenesis |
JUL 1999 |
19 pages |
| Authors:
Michael N. Gould; Philip Watson; WISCONSIN UNIV-MADISON
|
| The phenotype of two additional MMTV-neu N transgenic rats has been determined. Females of both of these lines do not display an abnormal phenotype. In contrast, males of line 6500 develop mammary carcinomas with an average latency of 387 days. This phenotype is highly penetrant, with 77.8% and 100% of the transgenic males carcinoma positive at 14 months and 18 months of age, respectively. Among those ... |
|
| Mechanisms Underlying the Very High Susceptibility of the Immature Mammary Gland to Carcinogenic Initiation |
JUL 1999 |
37 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| The overall goal of this project remains to explore the toxic effects of physical and chemical carcinogens on the immature mammary gland as compared to these effects on the young adult mammary gland using a rat model. During the third grant year we have: (1) completed all comparative cytotoxicity studies showing that the immature mammary gland is more sensitive to radiation and NMU but not DMBA, ... |
|
| Investigating the Role of Cooperative Interactions Between the neu Proto-oncogene and the Other erbB Family Members in Rat Mammary Carcinogenesis |
JUL 1998 |
24 pages |
| Authors:
Michael N. Gould; Philip Watson; WISCONSIN UNIV-MADISON
|
| Rats were created that are transgenic for the neu proto-oncogene in order to establish a rat model of neu-mediated mammary tumorigenesis. The mouse mammary tumor virus promoter drives the expression of neu. Three transgenic lines have been generated and one of these lines has been maintained homozygous for the transgene. To date, all three lines show a low incidence of spontaneous mammary tumorigenesis. The vast majority of tumors that have ... |
|
| Mechanisms Underlying the Very High Susceptibility of the Immature Mammary Gland to Carcinogenic Initiation |
JUL 1998 |
59 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| The overall goal of this project remains to explore the toxic effects of physical and chemical carcinogens on the immature mammary gland as compared to these effects on the young adult mammary gland using a rat model. During the second grant year we have: (1) shown that as with radiation, the rat mammary carcinogen NMU is more cytotoxic in the immature in situ mammary gland than it ... |
|
| Cloning and Characterizing Genes Involved in Monoterpene Induced MammaryTumor Regression |
MAY 1998 |
143 pages |
| Authors:
Michael N. Gould; Eric Ariazi; WISCONSIN UNIV-MADISON
|
| Monoterpene-induced/repressed genes were identified in regressing rat mammary carcinomas treated with dietary limonene using a newly developed method termed subtractive display. The subtractive display screen identified 42 monoterpene-induced genes comprising 9 known genes and 33 unidentified genes, as well as 58 monoterpene-repressed genes comprising 1 known gene and 57 unidentified genes. Several of the identified differentially expressed genes are involved in the TGFbeta signal tranduction pathway, ... |
|
| Mechanisms Underlying the Very High Susceptibility of the Immature Mammary Gland to Carcinogenic Initiation |
JUL 97 |
19 pages |
| Authors:
Michael N. Gould; WISCONSIN UNIV-MADISON
|
| The overall goal of this proposal is to explore the hypothesis that the diminished ability of mammary cells from immature rats to recover from cytotoxic radiation damage may extend to an increased susceptibility to mammary carcinogenesis. If so, such a mechanism may also underlie the observation that the immature breast of prepubertal human females is the developmental stage most highly susceptible to breast cancer initiation. It is further hypothesized that ... |
|
| Evaluating Gene Therapy Approaches for the Prevention of Breast Cancer |
JUL 97 |
40 pages |
| Authors:
Michael N. Gould; Philip Watson; WISCONSIN UNIV-MADISON
|
| In order to determine the feasibility of using gene therapy for breast cancer prevention in the rat, lacZ recombinant adenoviral and retroviral vectors were infused into the mammary glands of rats in situ. Staining of whole glands with X-gal revealed the location of infected cells. Adenovirus infected the end buds with high efficiency but failed to infect the primary/secondary ductal cells. Expression of lacZ from the adenoviral vector was transient, ... |
|
| Cloning and Characterizing Genes Involved in Monoterpene Induced Mammary Tumor Regression |
OCT 96 |
53 pages |
| Authors:
Michael N. Gould; Eric A. Ariazi; WISCONSIN UNIV-MADISON
|
| Monoterpene-induced/repressed genes were identified in regressing rat mammary carcinomas treated with dietary limonene using a newly developed method termed subtractive display. The subtractive display screen identified 42 monoterpene-induced genes comprising 9 known genes and 33 unidentified genes, as well as 58 monoterpene-repressed genes comprising 1 known gene and 57 unidentified genes. Several of the identified differentially expressed genes are involved in the mitoinhibitory transforming growth factor B signal tranduction pathway, ... |
|
| Comparative Roles of Overexpressed and Mutated H- and K-ras in Mammary Carcinogenesis |
AUG 1996 |
44 pages |
| Authors:
Michael N. Gould; Todd A. Thompson; WISCONSIN UNIV-MADISON
|
| The objective of this study was to characterize events in the uniformly selective association of H-ras gene activation, but not K-ras gene activation, in the rat mammary carcinogenesis model. Three primary objectives were investigated. First, the relative tumorigenic potency of activated H-ras and K-ras-2B was investigated. The activated H-ras gene was found to be approximately 10-fold more potent at inducing mammary tumors than the activated K-ras gene. Yet, the K-ras ... |
|
| Comparative Roles of Overexpressed and Mutated H-ras and K-ras in Mammary Carcinogenesis |
31 AUG 95 |
15 pages |
| Authors:
Michael N. Gould; Todd A. Thompson; WISCONSIN UNIV-MADISON
|
| The objective of this study is to characterize cellular and molecular events of chemically induced rat mammary carcinogenesis by asking why is selective activation of the Harvey ras gene, but not other similar members of the ras gene family, associated with this model. This study is divided into three main objectives. First, the ability of the activated and wild-type forms df Harvey ras and ... |
|
Reports by Author
