| Genetics Risk Factor for Prostate Cancer |
JAN 2004 |
15 pages |
| Authors:
Edward P. Gelmann; GEORGETOWN UNIV WASHINGTON DC
|
 | NKX3.l is a homeoprotein with prostate-specific expression in adults. Loss of NKX3.l correlates with prostate cancer progression. NKX3.l protein expression is reduced to varing degrees in virtually all primary prostate cancers. The NKX3.l gene is affected by deletion and/or promoter hypermethylation in 90% of primary prostate cancers. NKX3.l acts as a transcription factor by binding directly to DNA. NKX3.l also complexes and coactivates other transcription factors such as serum response ... |
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| Gene Methylation in Prostate Cancer |
JAN 2003 |
11 pages |
| Authors:
Huang Wen-Xin; Edward P. Gelmann; AMERICAN UNIV WASHINGTON DC
|
| Background: NKX3. 1 is a homeoprotein with prostate-specific expression in adults. Loss of NKX3.1 correlates with prostate cancer progression. Loss of heterozygosity affects NKK3.1 in about 80% of prostate cancers. This project focuses on DNA methylation of the NKK3.1 gene promoter in prostate cancer cell lines and tissues. Materials and methods: DNA was analyzed by methylation-specific PCR and sequencing of bisulfite-treated DNA. We also treated cultured cells with the methylation ... |
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| NKX3.1 in Prostate Cancer |
JAN 2003 |
86 pages |
| Authors:
Edward P. Gelmann; GEORGETOWN UNIV WASHINGTON DC
|
| NKX3.1 is a prostate-specific homeobox gene that undergoes loss of heterozygosity in -85% of prostate cancers, but one normal gene copy is retained in tumor cells. Haploinsuffiency of murine NKX3.1 is sufficient to permit prostatic hyperplasia and dysplasia later in life. Consistent with the findings in mouse models human NKX3.1 protein expression is substantially reduced in 80- 90% of primary prostate cancer. Complete loss of NKX3.1 expression is seen mostly ... |
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| Genetic Risk Factor for Prostate Cancer |
JAN 2003 |
21 pages |
| Authors:
Edward P. Gelmann; GEORGETOWN UNIV WASHINGTON DC
|
| NKX3.1 is a homeoprotein with prostate-specific expression in adult mammals. NKX3.1 gene targeting studies in mice have shown that the protein is a differentiating factor and suppressor of epithelial cell growth. Importantly, haploinsufficiency in the mice was dominant, suggesting that altered levels of protein could impart a phenotype in humans. We have described a genetic polymorphism, C154T, in 10% of the population that alters the amino acid sequence of the ... |
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| Phase I NKX3.1 in Prostate |
JAN 2001 |
13 pages |
| Authors:
Edward P. Gelmann; GEORGETOWN UNIV WASHINGTON DC
|
| We have isolated a prostate-specific gene, NKX3.1, that maps to chromosome 8p21, a common region for loss of heterozygosity in human prostate cancer. NKX3.1 is a homeobox gene that is expressed at high levels in adult human prostate. Deletion of NKX3.1 in the mouse has suggested that NKX3.1 has a suppressor effect on prostatic epithelial cell growth, and that haploinsufficiency is sufficient to produce epithelial hyperplasia. Inconsistent with the interpretation ... |
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| NKX3.1 In Prostate Cancer |
JUL 1999 |
10 pages |
| Authors:
Edward P. Gelmann; GEORGETOWN UNIV WASHINGTON DC
|
| We have isolated a prostate-specific gene, NKX3.1, that maps to chromosome 8p21, a common region for loss of heterozygosity in human prostate cancer. NKX3.1 is a homeobox gene that is expressed at high levels in adult human prostate. Deletion of NKX3.1 in the mouse has suggested that NKX3.1 has a suppressor effect on pro static epithelial cell growth, and that haploinsufficiency is sufficient to ... |
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