The individual and combined effects of sucrose laurate (SL, L-1695), tert-butylated hydroxyanisole (B) ethylenediamine tetraacetate (E) and nisin were evaluated in a liquid growth medium (antibiotic assay medium + 1% soluble starch, AAMS) to determine their antagonistic action against the thermophilic flat-sour bacterium, B. stearothermophilus. Results indicated that on AAMS agar (pH 6.5 - 6.8) the minimum inhibitory concentration (MIC) of these additives required to prevent germination and outgrowth of ...