| Ricin Activity Assay by Direct Analysis in Real Time Mass Spectrometry Detection of Adenine Release |
01 Feb 2010 |
4 pages |
| Authors:
Vicky L Bevilacqua; J M Nilles; Jeffrey S Rice; Theresa R Connell; Amanda M Schenning; Lisa M Reilly; H D Durst; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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 | Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, multistep analysis, or a combination thereof. We report a single-step, real time ricin activity assay that requires little or no sample preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by ricin was determined to be 53 +/- 2 pmol adenine per picomole of ricin per hour. This ... |
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| Development of a Versatile Conditioning Wind Tunnel for Evaporative Fate Studies |
Oct-2009 |
63 pages |
| Authors:
H D Durst; James E Danberg; Daniel J Weber; Daniel Waysbort; Clayton S Moury; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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| This report documents the development of a conditioning wind tunnel that allows multiple test samples to be conditioned at identical environmental settings that are used in smaller vapor sampling wind tunnels. The conditioning of multiple samples, especially those involving persistent chemicals, allows for the efficient use of a limited number of instrumented vapor sampling tunnels. Up to nine samples can be sequentially and quickly cycled through the instrumented vapor wind ... |
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| Evaporation Rates of Chemical Warfare Agents Measured Using 5 CM Wind Tunnels. 2. Munitions Grade Sulfur Mustard From Sand |
Jul-2009 |
184 pages |
| Authors:
H D Durst; Erin L Maloney; Christopher V Giannaras; Bruce E King; Joseph P Myers; Robert G Nickol; Seok H Hong; Kenneth B Sumpter; Carol A Brevett; John J Pence; SCIENCE APPLICATIONS INTERNATIONAL CORP GUNPOWDER MD
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| The evaporation of sulfur mustard from sand was studied as a function of temperature, drop size and air flow rate, using the same instrumentation as prior studies on glass. The evaporation rate increased with higher temperature, drop size, and wind speed; and an empirical equation was determined that would allow for the calculation of the evaporation rate given the environmental conditions. |
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| Mercapto-Oximes as Capture Molecules for Detection of Nerve Agents Using Surface-Enhanced Raman Spectroscopy |
Jun-2009 |
17 pages |
| Authors:
H D Durst; Kato L Killops; Roderick A Fry; William R Creasy; Jason A Guicheteau; CALIFORNIA UNIV SANTA BARBARA
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| Two compounds, 4-methylthiobenzaldehyde oxime and 4-thiobenzaldehyde oxime, were synthesized for use as capture molecules for applications using surface-enhanced Raman as a means of detection. The thioether moiety was successfully attached to the silver nanoparticle substrate as evidenced by the change in the resulting spectrum. The oxime was shown to be reactive toward the nerve agent GD (Soman) in basic solution, as monitored by (31)P NMR. |
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| Vapor-Phase Infrared Absorptivity Coefficient of 2-ChlorovinylDichloroarsine (LEWISITE) |
Jan-2009 |
23 pages |
| Authors:
Miles; Alan C Samuels; H D Durst; Ronald W Jr; Barry R Williams; Melissa S Hulet; Frederic J Berg; Leslie McMahon; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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| We measured the vapor-phase absorptivity coefficient of the vesicant chemical warfare agent 2-chlorovinyl dichloroarsine (Lewisite) in the mid-infrared (4000-550/cm) at a spectral resolution of 0.125/cm. The Lewisite used in the feedstock was purified by fractional distillation and analyzed by nuclear magnetic resonance to verify its purity. We describe the experimental method used to acquire the individual spectra that were used to produce the composite spectrum, summarize the statistical uncertainties in ... |
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| Evaporation Rates of Chemical Warfare Agents Using 5-CM Wind Tunnels I. CASARM Sulfur Mustard (HD) from Glass |
Oct-2008 |
187 pages |
| Authors:
John Pence; Aaron Flowers; Chris V Giannaras; Erin Maloney; Joseph P Myers; Robert G Nickol; Kenneth B Sumpter; Carol A Brevett; H D Durst; Bruce E King; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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| The evaporation of sulfur mustard (HD) from glass was studied as a function of temperature, drop size, and airflow rate. The evaporation rate was measured by collecting the HD vapor using thermal desorption tubes that were connected to the wind tunnel. The evaporation rate increased with higher temperature, drop size, and wind speed. Five different wind tunnels were used, and it was possible to fit all of the data generated ... |
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| Vapor-Phase Infrared Absorptivity Coefficient of BIS-(2-Chloroethyl) Sulfide |
01-Jul-2008 |
31 pages |
| Authors:
Alan C Samuels; H D Durst; Barry R Williams; Melissa S Hulet; Ronald W Miles; Frederic J Berg; Leslie McMahon; SCIENCE APPLICATIONS INTERNATIONAL CORP (SAIC) ABINGDON MD
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| We measured the vapor-phase absorptivity coefficient of the vesicant chemical warfare agent, bis-(2-chloroethyl) sulfide (HD), in the mid-infrared (4000-550/cm) at a spectral resolution of 0.125/cm. The HD used in the feedstock was subjected to a rigorous analysis by gas chromatography (GC) and NMR to verify its purity, and the vapor used to produce the composite spectrum was periodically sampled by GC. In this report, we describe the experimental method employed ... |
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| Fluoride Ion Regeneration of Sarin (GB) From Minipig Tissue and Fluids Following Whole-Body GB Vapor Exposure |
01-Jul-2003 |
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| Authors:
B J Benton; S A Thomson; H D Durst; J S Anthony; R J Mioduszewski; E M Jakubowski; K L Matson; S W Hulet; J H Manthei; J L Edwards; EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
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 | A method has been developed for the analysis of a Sarin (GB) nerve agent biomarker in the tissue and biological fluids of minipigs that is very sensitive and selective. The GB biomarker was the fluoride ion regenerated GB and was found in minipig samples such as red blood cells, brain, liver, lung, and adipose tissue after whole body inhalation exposure. Regenerated GB in the samples was analyzed by GC-FPD after ... |
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