| Proteomic Analysis of Bronchoalveolar Lavage Fluid: Effect of Acute Exposure to Diesel Exhaust Particles in Rats |
05 FEB 2007 |
39 pages |
| Authors:
John A. Lewis; K. M. Rao; Vince Castranova; Val Vallyathan; William E. Dennis; Paul L. Knechtges; ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD
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 | Inhalation of diesel exhaust particles (DEP) is characterized by lung injury and inflammation with significant increases in the numbers of polymorphonuclear leukocytes and alveolar macrophages. This influx of cellular infiltrates is associated with the activation of multiple genes, including cytokines and chemokines, and the production of reactive oxygen species. The pathogenesis of the lung injury is not fully understood, but alterations in the presence or abundance of a number of ... |
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| A Novel Amphibian Tier 2 Testing Protocol: A 30-Week Exposure of Xenopus Tropicalis to the Antiandrogen Flutamide |
2007 |
11 pages |
| Authors:
Paul L. Knechtges; Robert L. Sprando; Karen L. Porter; Linda M. Brennan; Mark F. Miller; David M. Kumsher; William E. Dennis; Charles C. Brown; Eric D. Clegg Paul L. Knechtges. Robert L. Sprando. Karen L. Potter.; ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD
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| In 1996, the U.S. Congress mandated the development of a screening program for endocrine-disrupting chemicals (EDCs) using validated test systems. Subsequently, the Endocrine Disruptor Screening and Testing Advisory Committee recommended the development of a standardized amphibian assay for tier 2 testing of EDCs. For that reason, a tier 2 testing protocol using Xenopus (Silurana) tropicalis and a 30-week, flow-through exposure to the antiandrogen flutamide from stage 46 tadpoles through sexually ... |
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| Assessment of Protocol Designed to Detect Endocine Disrupting Effects of Flutamide in Xenopus Tropicalis |
JAN 2006 |
45 pages |
| Authors:
Paul L. Kenchtges; Robert L. Sprando; Linda M. Bremman; Mark F. Miller; David M. Kumsher; William E. Dennis; Charles C. Brown; Eric D. Clegg; ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD
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| This technical report represents a pilot study to test a new protocol with an amphibian frog species, Xenopus tropicalis, for possible application in the U.S. Environmental Protection Agency (USEPA) Endocrine Disruptor Screening and Testing Program. The frogs were exposed to the model anti-androgenic compound flutamine under flow-through conditions for a period of 30 weeks, beginning at 48 hours post-hatch. The endpoints evaluated for this study were (1) overall health of ... |
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