Techiniques were developed for: (1) measuring the lipase activities of human platelets by patterns of release of endogenous fatty acids, as opposed to the release of fatty acids from model substrates; (2) measuring the phospholipase A2 activity of human platelets by the release of arachidonic acid as well as by oxygen consumption; (3) assessing the metabolism of platelets and arteries in vivo. Primate platelets and arteries are incapable of converting ...
Storage of human platelets at 4C for 24 hours results in a considerable reduction in their oxidative activity towards fatty acids, glucose and selected Krebs cycle substrates. Pursuant to these observations, the authors have focused on energy metabolism and mitochondrial behavior during platelet storage. To this end methods have been set up for measuring oxidative phosphorylation, and developing an improved method for homogenization and fractionation of human platelets. The authors ...
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