Dogs with Dirofilaria immitis microfilaremia were obtained from three geographically distinct areas to determine whether divergent strains of the parasite might develop differently in the mosquito Aedes triseriatus Say. Because no significant differences were noted, results of these experiments were combined to give an overall view of development of D. immitis in Aedes triseriatus. An average of twenty microfilariae was found in the midgut and malpighian tubules of the mosquitoes ...
The study was designed to develop a method for sterilizing vitamin-fortified commercial diets for feeding germ-free and defined-flora rodents that would (a) require less time and (b) not destroy vitamins. Feed contaminated with Bacillus stearothermophilus and B. subtilis was sterilized in high-temperature, high-prevacuum autoclave and assayed for vitamin A, thiamine, and pantothenic acid. It was found that: (1) The use of high-temperature, high-prevacuum autoclaves makes possible a reduction in the ...
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