N. Chilukuri - Storming Media

Adenovirus-Mediated Human Paraoxonase 1 Gene Transfer to Provide Protection Against the Toxicity of the Organophosphorus Pesticide Toxicant Diazoxon

MAR 2011

1 pages

Authors: E. G. Duysen; K. Parikh; V. Aleti; V. Manne; O. Lockridge; N. Chilukuri; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF MOLECULAR PHARMACOLOGY

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Human paraoxonase1 (hPON1) is a potential therapeutic against the toxicity of organophosphorus (OP) pesticides and chemical warfare nerve agents. We tested whether PON1 gene transfer using adenovirus provides protection against the toxicity of the OP diazoxon. Using an adenovirus construct containing hPON1 gene, we showed elevated levels of recombinant enzyme was secreted by 293A cells into culture medium and into the systemic circulation of mice. Western blotting revealed that the ...

Application of Adenovirus Expression System for the Production of Recombinant Cholinesterases

01 OCT 2005

8 pages

Authors: N. Chilukuri; K. Parikh; R. Naik; C. Chambers; J. M. Watt; P. Tipparaju; B. P. Doctor; A. Saxena; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD

Previous studies in rodents and nonhuman primates demonstrated that pretreatment of animals with cholinesterases (ChEs) could provide significant protection against behavioral and lethal effects of nerve agent intoxication. Currently, human serum butyrylcholinesterase (BChE) is under development as medical countermeasure against organophosphate chemical warfare agent toxicity. In this study, we report the expression of human BChE and bovine acetylcholinesterase (AChE) using the newly designed adenovirus expression system. The shuttle vector employed ...

Role of Pegylation on the Circulatory Stability and Immunogenicity of Recombinant Cholinesterases

17 NOV 2004

10 pages

Authors: N. Chilukuri; W. Sun; R. Naik; P. Tipparaju; K. Parikh; C. Luo; B. P. Doctor; A. Saxena; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD

Previous studies in rodents and non human primates demonstrated that pretreatment of animals with cholinesterases (ChEs), both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), could provide significant protection against behavioral and lethal effects of nerve agent intoxication. Currently, human (Hu) serum BChE is under development as a medical countermeasure against organophosphate chemical warfare agent toxicity. Similarly, methods for producing gram quantities of recombinant (r)Hu BChE and its development as a bioscavenger are ...