| Early Transcriptional Responses of HepG2-A 16 Liver Cells to Infection by Plasmodium falciparum Sporozoites |
29 Jul 2011 |
11 pages |
| Authors:
Rana Chattopadhyay; Patricia de la Vega; Sun H Paik; Yoko Murata; Earl W Ferguson; Thomas L Richie; Guck T Ooi; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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 | Invasion of hepatocytes by Plasmodium sporozoites deposited by Anopheles mosquitoes, and their subsequent transformation into infective merozoites is an obligatory step in the initiation of malaria. Interactions between the sporozoites and hepatocytes lead to a distinct, complex and coordinated cellular and systemic host response. Little is known about host liver cell response to sporozoite invasion, or whether it is primarily adaptive for the parasite, for the host, or for both. ... |
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| Establishment of an In Vitro Assay for Assessing the Effects of Drugs on the Liver Stages of Plasmodium vivax Malaria |
Jun 2010 |
10 pages |
| Authors:
Rana Chattopadhyay; Soundarapandian Velmurugan; Chinnamma Chaliath; William E Collins; Stephen L Hoffman; WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD
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| Plasmodium vivax (Pv) is the second most important human malaria parasite. Recent data indicate that the impact of Pv malaria on the health and economies of the developing world has been dramatically underestimated. Pv has a unique feature in its life cycle. Uninucleate sporozoites (spz), after invasion of human hepatocytes, either proceed to develop into tens of thousands of merozoites within the infected hepatocytes or remain as dormant forms called ... |
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| Multiple Antigen Peptide Vaccines against Plasmodium falciparum Malaria |
Jan 2010 |
13 pages |
| Authors:
Babita Mahajan; Jay A Berzofsky; Robert A Boykins; Victoria Majam; Hong Zheng; Rana Chattopadhyay; Patricia de la Vega; J K Moch; J D Haynes; Igor M Belyakov; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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| The multiple antigen peptide (MAP} approach is an elfective method to chemically synthesize and deliver multiple T-cell and 8-cell epitopes as the constituents of a single immunogen. Here we report on the design, chemical synthesis, and immunogenicity of three PIDsmodium falcipamm MAP vaccines that incorporated antigenic epitopes from the sporozoite, liver, and blood stages of the life cycle. Antibody and cellular responses were determined in three inbred (C5781)6, BALB/c, and ... |
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| Development of a Metabolically Active, Non-Replicating Sporozoite Vaccine to Prevent Plasmodium falciparum Malaria |
Oct 2009 |
11 pages |
| Authors:
Stephen L Hoffman; Peter F Billingsley; Eric James; Adam Richman; Mark Loyevsky; Tao Li; Sumana Chakravarty; Anusha Gunasekera; Rana Chattopadhyay; Minglin Li; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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| Immunization of volunteers by the bite of mosquitoes carrying radiation-attenuated Plasmodium falciparum sporozoites protects greater than 90% of such volunteers against malaria, if adequate numbers of immunizing biting sessions and sporozoite- infected mosquitoes are used. Nonetheless, until recently It was considered impossible to develop, license and commercialize a live, whole parasite P. falciparum porozoite (PfSPZ) vaccine . In 2003 Sanaria scientists reappraised the potential impact of a metabolically active , ... |
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| Identification, Cloning, Expression, and Characterization of the Gene for Plasmodium knowlesi Surface Protein Containing an Altered Thrombospondin Repeat Domain |
27 APR 2005 |
9 pages |
| Authors:
Babita Mahajan; Dewal Jani; Rana Chattopadhyay; Rana Nagarkatti; Hong Zheng; Victoria Majam; Walter Weiss; Sanjai Kumar; Dharmendar Rathore; FOOD AND DRUG ADMINISTRATION ROCKVILLE MD
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| Proteins present on the surface of malaria parasites that participate in the process of invasion and adhesion to host cells are considered attractive vaccine targets. Aided by the availability of the partially completed genome sequence of the simian malaria parasite Plasmodium knowlesi, we have identified a 786-bp DNA sequence that encodes a 262-amino-acid-long protein, containing an altered version of the thrombospondin type I repeat domain (SPATR). Thrombospondin type 1 repeat ... |
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| Novel Antigen Identification Method for Discovery of Protective Malaria Antigens by Rapid Testing of DNA Vaccines Encoding Exons from the Parasite Genome |
MAR 2004 |
10 pages |
| Authors:
Diana Haddad; Erika Bilcikova; Adam A. Witney; Jane M. Carlton; Charles E. White; Peter L. Blair; Rana Chattopadhyay; Joshua Russell; Esteban Abot; Yupin Charoenvit; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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| We describe a novel approach for identifying target antigens for preerythrocytic malaria vaccines. Our strategy is to rapidly test hundreds of DNA vaccines encoding exons from the Plasmodium yoelii yoelii genomic sequence. In this antigen identification method, we measure reduction in parasite burden in the liver after sporozoite challenge in mice. Orthologs of protective P. y. yoelii genes can then be identified in the genomic databases of Plasmodium falciparum and ... |
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| Induction in Humans of CD8+ and CD4+ T Cell and Antibody Responses by Sequential Immunization with Malaria DNA and Recombinant Protein |
13 FEB 2004 |
10 pages |
| Authors:
Ruobing Wang; Judith Epstein; Yupin Charoenvit; Fe Maria Baraceros; Nancy Rahardjo; Tanya Gay; Jo-Glenna Banania; Rana Chattopadhyay; Patricia de la Vega; Thomas L. Richie; NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD
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| Vaccine-induced protection against diseases like malaria, AIDS, and cancer may require induction of Ag-specific CD8 and CD4 T cell and Ab responses in the same individual. In humans, a recombinant Plasmodium falciparum circumsporozoite protein (PfCSP) candidate vaccine, RTS,S/adjuvant system number 2A (AS02A), induces T cells and Abs, but no measurable CD8 T cells by CTL or short-term (ex vivo) IFN-ELISPOT assays, and partial short-term protection. P. falciparum DNA vaccines elicit ... |
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