This study took several approaches to determine the influence of a single nucleotide polymorphism (SNP) in the matrix metalloproteinase 1 promoter on gene transcription in normal and breast cancer cell lines or tissue. The SNP consists of an extra guanine nucleotide that creates an ETS family transcription factor binding site (2G), and we previously demonstrated that the 2G SNP leads to an increase in transcriptional activity of the MMP-l promoters ...
Tumor invasion requires destruction of collagen, and is accomplished by matrix metalloproteinase-l (MMP-1). A single nucleotide polymorphism (SNP) in the MMP-1 promoter enhances transcription of this gene. The SNP is at - 1607 bp in the MMP-1 promoter, where an additional guanine (G) creates a binding site (5-AGGA-3 ) for the Ets transcription factors. Allele frequency is: 25% =1 G homozygous, 25% =2 G homozygous, and 50% = 1G/2G heterozygous. ...
The lethality of breast cancer is derived from its ability to metastasize, and matrix metalloproteinases (MMPs) facilitate cellular invasion by degrading the extracellular matrix. One component of MMP regulation is transcription. We have identified a single nucleotide polymorphism (SNP) that enhances the transcriptional activity of MMP-l promoters in transient transfections. The SNP consists of an extra guanine nucleotide that creates an ETS family transcription factor binding site (2G). We utilized ...
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