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Abstract:
This project will evaluate paclitaxel chemotherapy delivery from a gel polymer system placed into a wound bed following conservative surgical removal of human breast cancers grown in nude mice. This novel delivery method is proposed to control local tumor disease as well as assist in control of metastasis and may offer a cost-effective alternative to adjuvant radiation therapy. Task (objective) 1 (proposed to be completed in year 1): To evaluate the efficacy of polymer delivered paclitaxel chemotherapy against human breast tumor cell lines. As per task 1, we have established 5 human breast cancer cell lines within our laboratory; MCF-7, MCF-7 AL, MDA-MB-435, MDA-MB-231 and MX-1. We have elected to pursue purchase and implementation of a unique luciferase imaging system (not in original proposal) which will allow in vivo imaging of tumor growth and metastasis (and subsequently decrease animal use) . Use of this system requires transfection of the breast tumor cell lines with the luciferase gene. Thus far we have successfully transfected the MDA-MB-435 line and are in the process of transfecting the remaining cell lines. This has caused a slight delay in completion of task 1, however, we have recently completed in vitro testing of 4 of 5 of the cell lines for taxol sensitivity (results enclosed).
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 14 May 2002-13 May 2003 |
| Pages: |
11 |
| Report Date: |
JUN 2003 |
| Contract Number: |
DAMD17-02-1-0348 |
| Report Number: |
A839514 |
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