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Abstract:
The focus: Despite compelling cell biological studies and histopathological observations incriminating stromal cells in tumorigenesis, our knowledge of the genes that mediate changes in the tumor microenvironment and interactions among various cell types in epithelial cancer and their role in tumorigenesis is limited. Here, we describe a comprehensive molecular characterization of stromal-epithelial cell interactions, using microarray analysis of co-cultured cell pairs. We further show that these gene expression changes indeed are common feature of in vivo profiles of invasive epithelial cancers. We provide proof of principle evidence that the in vitro system recapitulates some aspects of cancer cell biology and may contribute new markers to the invasive events. We directly demonstrate that events in the interaction of epithelial cells and their neighboring fibroblasts play initial role in recruiting inflammatory cells and provoking immune surveillance. A reverse signal transduction approach: Perhaps the biggest innovation lies in the demonstrated ability to discern cytokine molecules that provoke the gene expression in the co-culture. This in turn provides new working hypotheses to test in vivo, on the etiological role of particular molecules in dictating the clinical course of the disease.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 1 Mr 2004-28 Feb 2005 |
| Pages: |
22 |
| Report Date: |
MAR 2005 |
| Contract Number: |
W81XWH0410336 |
| Report Number: |
A679344 |
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Keywords relating to this report:
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