|
Abstract:
The main goal of the research proposal was to develop an assay system for studying the specific genetic elements, if any, involved in the initiation of DNA replication in mammalian cells as outlined in Task 1 (development of assay system, see Appendix Statement of Work). The completion of this task is outlined in the annual summary from last year and led to the first peer-reviewed publication resulting from this research proposal (See Annual Summary, 2000, and attached reprint Altman and Fanning, 2001). Briefly, a competitive polymerase chain reaction (PCR)-based nascent strand abundance assay was used to demonstrate the ability of a small 5.8 kb fragment of DNA, containing the DHFR ori-beta initiation region (IR), to support efficient origin activity when integrated into random ectopic positions in the hamster chromosome (Altman and Fanning, 2001). In addition, the integrated ori-beta IR functioned with the same efficiency as the endogenous ori-beta in CHOK1 cells, suggesting that the 5.8 kb fragment was an acceptable candidate for mutational analysis of the ori-beta IR.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual summary rept. 1 Aug 2000-31 Jul 2001 |
| Pages: |
31 |
| Report Date: |
AUG 2001 |
| Contract Number: |
DAMD17-99-1-9420 |
| Report Number: |
A584004 |
|
|
|
|
|
Keywords relating to this report:
Email This Abstract
