 Similar reports available for purchase |  | |  |
|
 |
 |
 Electronics and Fluidics Electrooptical and Optoelectronic Devices |
 |
|
New Approaches Towards the Elucidation of Epidermal-Dermal Separation in Sulfur Mustard-Exposed Human Skin and Directions For Therapy
Authors: Marijke A. E. Mol; PRINS MAURITS LABORATORIUM TNO RIJSWIJK(NETHERLANDS) |
| |
Abstract: The first year of the research performed under Award Number DAMD17- 02-02O6 has yielded promising results. with respect to the application of MMP inhibitor BB94 on HD-exposed human skin pieces in order to prevent microvesication. BB94 is effective at a concentration of at least 1 mug/ml and can be added to the culture medium as late as 8 h after exposure to HD, without loosing its inhibiting effect on epidermal-dermal separation. From a therapeutic angle, this time window might allow non-urgent cure of HD casualties. In an adjacent series of experiments, application of proteomic analysis as an unbiased way to study cellular events following HD-exposure has proven to be a valuable approach. 2D-PAGE analysis of the protein profile of cultured HEK that were exposed to 100 muM HD gave clues for the induction of apoptosis by the gradual appearance over 24 h of fragments of the type I keratins K14 and K17, generated by caspase cleavage. It is expected that the identification of several other new spots that appeared in the protein pattern of HD-exposed HEK might shed more light on the mechanistic toxicology of HD. The results obtained in this study so far, suggest a dual treatment of HD-exposed skin: application of a MMP- inhibitor to prevent microvesication together with the use of a caspase- inhibitor to reduce epidermal cell damage.
| Description: |
Annual rept. 16 Dec 2001-16 Dec 2002 |
| Pages: |
19 |
| Report Date: |
JAN 2003 |
| Contract Number: |
DAMD17-02-1-0206 |
| Report Number: |
A583714 |
Report Unavailable |
| This title is unavailable from Storming Media. We do not know when it might be available, if at all. We list the report on our site for bibliographic completeness, to help our users know what other work has been performed in this field. Please note that as with all titles on this site, we do not have contact information for any of the authors. Nor can we give any suggestions on how one might obtain this report. |
|
|
|
|
|
|
| |
|
|
 |
|
 |
|
|
Keywords relating to this report:
Email This Abstract