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MedicineMedicine and Medical Research

Effect of Hydrostatic Pressure and Electroporation on Bactericidal Efficiency in Combination with Bacteriocins and Lysozyme. Phase I

Authors: Norasak Kalchayanand; Bibek Ray; Anthony Sikes; C. P. Dunne; WYOMING UNIV LARAMIE
Abstract:
The bactericidal efficiency of ultrahigh hydrostatic pressure (UMP) and pulsed electric field (PEF) were studied in combination with two acteriocins, nisin A and pediocin AcH, and lysozyme. Several Gram-positive and Gram-negative bacterial species and strains associated with food spoilage and foodborne diseases were tested. The results showed that both UHP and PEF inflict lethal and sublethal injury to Gram-positive and Gram-negative bacterial cells. As the sublethally-injured cells became sensitive to the bactericidal action of the two bacteriocins and lysozyme, the combination of the UHP and PEF treatment, along with bacteriocin and lysozyme resulted in greater viability loss than when only the UHP or PEF are used. In general, under the study conditions, UHP and bacteriocins produced more viability loss than PEF with and without bacteriocins. By combining bacteriocins and lysozyme, UHP at 30,000 psi for 1 min produced viability loss of 13.8 logs in Listeria monocytogenes Scott A and 6.3 logs in Salmonella typhimurium as compared to 2.8 logs for both species by UHP alone and 4.7 and 4.1 logs, respectively, by UHP + bacteriocins.

Limitations: APPROVED FOR PUBLIC RELEASE
Description: Final rept. Oct 93-Sep 94
Pages: 27
Report Date: SEP 96
Contract Number: DAAK60-93-K-0003
Report Number: A521513
Keywords relating to this report:
*ANTIMICROBIAL AGENTS
*GRAM NEGATIVE BACTERIA
*GRAM POSITIVE BACTERIA
*SALMONELLA TYPHIMURIUM
BACTERIA
DISEASES
ELECTRIC FIELDS
FOOD DETERIORATION
FOOD PRESERVATION
HYDROSTATIC PRESSURE
LETHALITY
LOSSES
MURAMIDASE
NUTRIENTS
PULSES
SUBLETHAL DOSAGE
VIABILITY
WOUNDS AND INJURIES
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