Abstract: This report is a required report for the U.S. Army Edgewood Chemical Biological Center In-House Laboratory Independent Research project Cell Toxicity by NMR. Traditional assays lead to IC50/EC50 values based solely on cell survivability. However, additional information is desirable for understanding a toxin's total effects. Complex cellular metabolite mixtures can be analyzed by nuclear magnetic resonance (NMR) with the aid of statistical methods such as chemometrics analysis (CA). However, the use of NMR-CA for cell toxicity to date has required model-building with IC50/EC50 values obtained from conventional cytotoxicity assays. Here we report on an exploratory study to determine the feasibility of using NMR-CA independently for the study of ricin toxicity in BALB/c 3T3 Murine Fibroblasts (CCL-163 American Type Culture Collection [ATCC], Manassas, VA) by analysis of the cell growth media alone. This work involves principal component analysis (PCA) as a chemometrics analysis tool. We have found that NMR-PCA readily distinguishes between multiple-component media samples from the same preparation that had cells grown in them from those that did not have cells grown in them. We also have preliminary evidence that this procedure will be able to identify differences between media from cells dosed with varying concentrations of ricin.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Final rept. Mar-Oct 2008 |
| Pages: |
16 |
| Report Date: |
Apr-2009 |
| Report Number: |
A520005 |
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