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Abstract:
Genetic changes implicated in the etiology of breast cancer have been identified by the detection of loss of heterozygosity at specific loci. Our study utilizes a series of genetic polymorphisms detectable by the polymerase chain reaction (PCR) to look for changing patterns of LOH as breast cancer progresses from intraductal to invasive and then to metastatic disease. The initial phases of this work involved the identification of cases from the AFIP archives, the development of procedures for cutting cases, and microdissection of individual tumor components. In the past year, lysates from 116 breast carcinomas have been analyzed for LOH at 11 markers on chromosome lip15, 5 on 17q, two on 3p, and two on 14q. Analysis of the lip 15 data localizes a common region of LOH to a region of approximately 9 megabases centered on the markers D11S1318 and D11S4046. LOH of chromosome 17 shows a more complex distribution which is being characterized through the use of multiple markers along this chromosome. LOH at the markers analyzed thus far is almost always seen in the intraductal carcinoma and maintained throughout the subsequent stages of tumor progression.
| Limitations: |
 APPROVED FOR PUBLIC RELEASE |
| Description: |
Annual rept. 30 Sep-29 Sep 95 |
| Pages: |
37 |
| Report Date: |
OCT 96 |
| Contract Number: |
DAMD17-94-J-4330 |
| Report Number: |
A464623 |
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