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Biological SciencesMicrobiology

Ultrasonic Disinfection of Water Suspensions of Escherichia Coli and Legionella Pneumophila

Authors: Victor F. Evora; George J. Kavarnos; NAVAL UNDERSEA WARFARE CENTER NEWPORT DIV RI
 
Abstract: This report describes an experiment in which water samples inoculated with Escherichia (E.) coil and Legionella (L.) pneumophila organisms were exposed to ultrasonic energy. It was found that treating these samples with pulses of ultrasound for at least 15 minutes at a 50% amplitude virtually eliminated the E. coil colonies. Longer exposure times were required to completely destroy L. pneumophila. Although the water samples experienced slight warming during the course of the ultrasonification, control experiments (in which the test samples were not exposed to ultrasound but simply warmed) have supported the conclusion that the test organisms were destroyed by acoustic energy alone. The use of ultrasound as an applied and commercial technology for disinfecting water supplies is also discussed in the report. However, the mechanisms of cell destruction, not yet fully understood, are left for the subject of a future publication.

Limitations: APPROVED FOR PUBLIC RELEASE
Description: Final rept.
Pages: 18
Report Date: 05 APR 1999
Report Number: A444563
Keywords relating to this report:
ACOUSTIC WAVES
CELLS(BIOLOGY)
COILS
COLONIES(BIOLOGY)
COMMERCE
CONTROL
DESTRUCTION
DISINFECTION
ENERGY
ESCHERICHIA COLI
EXPOSURE(GENERAL)
HEATING
LEGIONELLA PNEUMOPHILA
PULSES
SAMPLING
TEST AND EVALUATION
ULTRASONICS
WATER
WATER SUPPLIES
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