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Biological SciencesMicrobiology

Ultrafiltration as a Method for Concentrating Rift Valley Fever Grown in Tissue Culture

Authors: Frederick Klein; Bill G Mahlandt; Hobert B Bonner; RALPH E LINCOLN; FORT DETRICK FREDERICK MD
Abstract:
Filtration by means of a Diaflo ultrafilter was used to concentrate three 1,000-ml lots and one 3,000-ml lot of tissue culture-grown Rift Valley fever virus. Quantitation of both infectivity and total protein was achieved. Water treatment with continued ultrafiltratin of the virus concentrate provided a final virus product approximately 99.25% free of low-molecular-weight materials originally present in the growth medium.

Limitations: APPROVED FOR PUBLIC RELEASE
Pages: 4
Report Date: 09 Dec 1970
Report Number: 0368427
Keywords relating to this report:
ARBOVIRUSES
CONCENTRATION(CHEMISTRY)
FLUID FILTERS
PURIFICATION
RIFT VALLEY FEVER VIRUS
VIRUSES
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